Stability determination of candidate reference genes in cucumber plants subjected to stresses from Phytophthora melonis

Abstract

Stable reference genes are indispensable for ensuring the fi delity of determined gene expression levels. However, the expression levels of reference genes are unable to remain constant under all possible experimental conditions. Therefore, the stability determination of reference genes is necessary in an experimental system set. In the preset study, the stability of nine cucumber candidate reference genes (CsACT, CsUBQ, CsEF1α, CsCYP, CsαTU, CsCACS, CsTIP41, CsYSL8 and CsHEL) subjected to stresses from Phytophthora melonis (P. melonis) were determined using four different analysis methods, including Delta Ct, BestKeeper, NormFinder and GeNorm. The study results revealed that CsUBQ and CsCYP were the most stable genes suitable as internal control in cucumber plants under attack by P. melonis condition.

Functional and Structural Analysis of a Novel Acyltransferase from Pathogenic Phytophthora melonis

“… 6 The disease is progressing each year in the damp and humid environment of Guangzhou and costs millions of dollars to commercial cucumber production. 7 The pathogen is responsible for a disease called cucumber blight, which not only affects the yield (up to 80%) but also the quality of the produce, severely declining its market value. Because the pathogen already has a wide host range along with the tendency to infect more crops, it poses a biosecurity threat to the local agricultural system.…”

“…Because the pathogen already has a wide host range along with the tendency to infect more crops, it poses a biosecurity threat to the local agricultural system. 7 , 8 An acyltransferase (H3GZF6) was detected as a pathogenicity protein in our research project funded by Dean Funds of the Guangdong Academy of Agricultural Sciences (201818B), aimed to discover the pathogenicity factors of P. melonis . The protein was also the cause of sudden oak death.…”

“…Phytophthora melonis is a devastating plant pathogen and attacks a wide range of important plants (agricultural and ornamental), including cucumber, Cucumis sativus . The disease is progressing each year in the damp and humid environment of Guangzhou and costs millions of dollars to commercial cucumber production . The pathogen is responsible for a disease called cucumber blight, which not only affects the yield (up to 80%) but also the quality of the produce, severely declining its market value.…”

“…The pathogen is responsible for a disease called cucumber blight, which not only affects the yield (up to 80%) but also the quality of the produce, severely declining its market value. Because the pathogen already has a wide host range along with the tendency to infect more crops, it poses a biosecurity threat to the local agricultural system. , An acyltransferase (H3GZF6) was detected as a pathogenicity protein in our research project funded by Dean Funds of the Guangdong Academy of Agricultural Sciences (201818B), aimed to discover the pathogenicity factors of P. melonis.…”