Stability and networks of hydrogen bonds of the collagen triple helical structure: influence of pH and chaotropic nature of three anions

Molecular Characteristics of Acid and Pepsin Soluble Collagens From the Scales of Golden Carp (Probarbus Jullieni)

“…The hydrogen bond between N-H stretch (X position) and C=O (Gly) of the fourth residue is accountable for the formation of triple helix (Zanaboni et al, 2000). The higher wavenumber observed in ASC suggested that the removal of telopeptide could lower the C=O of PSC.…”

Photothermal-modulated drug delivery and magnetic relaxation based on collagen/poly(γ-glutamic acid) hydrogel

“…11,14,15 Collagens are one of the major hydrogel component materials studied as drug delivery carriers and scaffolds for cell therapy. [16][17][18][19][20] They are the major structural proteins of most connective …”

“…The fibril structures were well-dispersed in aqueous solution at low temperature, while they formed a gel at elevated temperature via the aggregation of the fibril structure. 16,17 To improve the weak mechanical properties and rapid degradation characteristics of collagen, high-concentration collagen solutions and cross-linked collagen gels are used in various applications. 21,22 However, the high viscosity and gelation properties of highly concentrated collagen solutions at room temperature limit their use.…”

“…These results suggest that the intermolecular hydrogen bond interactions between triple helices in collagen were completely disrupted, and the collagen gel structure could not sustain the gel structure at 42°C. 16,17 In fact, collagens are known to form highly viscous solutions via strong hydrogen bonding interactions between triple helices in addition to water-mediated hydrogen bonding between water molecules and the triple helices. 16,17 To investigate the role of hydrogen bonding interaction in the gelation of collagen hydrogel, we used urea as a hydrogen bonding inhibitor.…”

“…16,17 In fact, collagens are known to form highly viscous solutions via strong hydrogen bonding interactions between triple helices in addition to water-mediated hydrogen bonding between water molecules and the triple helices. 16,17 To investigate the role of hydrogen bonding interaction in the gelation of collagen hydrogel, we used urea as a hydrogen bonding inhibitor. 28,29 In the presence of high urea concentrations, the collagen/γ-PGA hydrogel began to break down and became transparent even at body temperature (Figure 2).…”