Stability and biodegradation of silk fibroin/hyaluronic acid nerve conduits

Hu, Zhanao; Das, Sourav Kumar; Yan, Shuqin; You, Renchuan; Li, Xiufang; Luo, Zuwei; Li, Mingzhong; Zhang, Qiang; Kaplan, David L.

doi:10.1016/j.compositesb.2020.108222

Cited by 39 publications

(21 citation statements)

References 37 publications

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“…In silk-based materials, the α-form usually contains characteristic absorption bands in the ranges of 1650–1660 cm −1 (amide I), 1545–1550 cm −1 (amide II), and 1270–1275 cm −1 (amide III). On the other hand, β-layers have characteristic bands in the ranges of 1620–1635 cm −1 (amide I), 1515–1530 cm −1 (amide II) and 1220–1245 cm −1 (amide III), 43 showing that different curing times induced a transition to a β-sheet structure in all hydrogel batches containing silk fibroin. The β-layer formation may increase with increasing curing time.…”

Section: Resultsmentioning

confidence: 98%

Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treatment of corneal perforations

et al. 2022

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Section: Resultsmentioning

confidence: 98%

Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treatment of corneal perforations

et al. 2022

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“…The pristine SF exhibited characteristic peaks at 3300 cm −1 (-NH stretching group), 1636 cm −1 (amide I), 1556 cm −1 (amide II), and 1232 cm −1 (amide III) [42]. SF crosslinked with EDC-NHS (ENSF) showed a strong peak at 1716 cm −1 , an amide group that confirms chemical crosslinking between the carboxyl group of SF and the amine group of EDC [43]. The 0.3% GG/3.7% SF/CS, 0.5% GG/3.5% SF/CS, 0.75% GG/3.25% SF/CS, and 1% GG/3% SF/CS hydrogels displayed characteristic peaks at 3302 cm −1 , 3302 cm −1 , 3306 cm −1 , and 3303 cm −1 (-OH and -NH stretching); 1634 cm −1 , 1634 cm −1 , 1633 cm −1 , and 1634 cm −1 (amide I); 1557 cm −1 , 1557 cm −1 , 1563 cm −1 , and 1563 cm −1 (amide II); 1221 cm −1 , 1227 cm −1 , 1229 cm −1 , and 1226 cm −1 (amide III); and 1026 cm −1 , 1030 cm −1 , 1029 cm −1 , and 1029 cm −1 (-COC stretching).…”

Section: Ft-ir Analysismentioning

confidence: 86%

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

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Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

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“…Phosphate buffer saline was refreshed every 3 days. At the specific time points, the samples were rinsed with distilled water and dried in a desiccator for 24 h (Hu et al., 2020). The weight loss of membranes was calculated according to Equation (2) (Atrian et al., 2019a, 2019b):

\mathrm{W}\mathrm{e}\mathrm{i}\mathrm{g}\mathrm{h}\mathrm{t}\,\mathrm{l}\mathrm{o}\mathrm{s}\mathrm{s}\,(\%)=\left[\frac{{\mathrm{W}}_{1}-{\mathrm{W}}_{2}}{{\mathrm{W}}_{1}}\right]\times 100

…”

Section: Methodsmentioning

confidence: 99%

“…Phosphate buffer saline was refreshed every 3 days. At the specific time points, the samples were rinsed with distilled water and dried in a desiccator for 24 h (Hu et al, 2020). The weight loss of membranes was calculated according to Equation (2) (Atrian et al, 2019a(Atrian et al, , 2019b:…”

Section: Characterization Of Keratin-modified Sf-5lap Membranesmentioning

confidence: 99%

Zwitterionic keratin coating on silk‐Laponite fibrous membranes for guided bone regeneration

Atrian

Kharaziha

Javidan

et al. 2022

J Tissue Eng Regen Med

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Implant-related infection is one of the main challenges in periodontal diseases.According to the zwitterionic properties of keratin, we aim to develop guided bone regeneration (GBR) membrane with antibacterial and bioactivity properties using a keratin coating. In this study, electrospun silk fibroin (SF)-Laponite (LAP) fibrous membranes were developed as GBR membranes, and keratin extracted from sheep wool was electrosprayed on them. Here, the role of electrospraying time (2, 3, and 4h) on the properties of the GBR membranes was investigated. After physicochemical characterization of the keratin-modified membranes, in vitro bioactivity and degradation rate of the membranes were studied in simulated body fluid and phosphate buffer saline, respectively. Moreover, proliferation and differentiation of mesenchymal stem cells were evaluated in contact with the keratin-modified SF-LAP membrane. Finally, the antibacterial activity of membranes against grampositive bacteria (Staphylococcus aureus) was investigated. Results demonstrated the successful formation of homogeneous wool keratin coating on SF-LAP fibrous membranes using a simple electrospray process. While wool keratin coating significantly enhanced the elongation and hydrophilicity of the SF-LAP membrane, the mechanical strength was not changed. In addition, keratin coating significantly improved the bioactivity and degradation rate of SF-LAP membranes, owing to the carboxyl groups of amino acids in keratin coating. In addition, the synergic role of LAP nanoparticles and keratin coating drastically improved osteoblast proliferation and differentiation. Finally, the zwitterionic property of wool keratin coating originating from their equal positive (NH 3 + ) and negative (COO − ) charges considerably improved the antibacterial activity of the SF-LAP membrane. Overall, keratincoated SF-LAP fibrous membranes with significant mechanical and biological properties could have the potential for GBR membranes.

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Stability and biodegradation of silk fibroin/hyaluronic acid nerve conduits

Cited by 39 publications

References 37 publications

Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treatment of corneal perforations

Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treatment of corneal perforations

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Zwitterionic keratin coating on silk‐Laponite fibrous membranes for guided bone regeneration

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