Stabilisation and immobilisation of penicillin amidase

Abstract

Pemcdlm amldase was coupled to a penodate-oxrdtsed dextran by reducttve alkylatron m the presence of sodmm cyanoborohydnde A loss of acttvtty (25%) was observed but the conIugate enzyme dextran was more thermostable than the native enzyme Native and dextran-conjugated pemctlhn am&se were rmmobthsed on ammo acttvated sthca (Promaxon, Spherostl, Aerostl) by a classical method usmg ~ut~aldehyde for the nattve enzyme and reducttve alkylatton for the mod&cd enzyme Good relanve acttvrty of the enzymes was obtained after ln~lubill~tion Immobthsatton of both native and mod&d enzymes resulted m the the~ostablhsatlon of the pemcdhn amtdase Enzyme stabrhsatton, Enzyme tmmobthsatton, Pemcdhn amrdase, (Escherrchm co/i)

Circular dichroism analysis of penicillin G acylase covalently immobilized on silica nanoparticles

“…The CD spectrum of free penicillin G acylase was first determined by Lindsay and Pain [24], which was taken in this work as reference. Covalent immobilization of penicillin G acylase on solid supports was accomplished by several working groups [25,26] and resulted in improvements similar to those described above. In our study, covalent immobilization was achieved by functionalizing the silica nanoparticles with glutardialdehyde and coupling to the free NH 2 groups of the enzyme (lysine and arginine side chains).…”

Common Causes of Glucose Oxidase Instability in In Vivo Biosensing: A Brief Review

“…López-Gallego and coauthors 24 suggested that a key factor involves the use of supports that are preactivated [35][36][37][38][39] versus nonactivated supports. 40,41 Preactivation allows only primary amino groups of the enzyme to react with the aldehyde groups introduced by modification of the amino groups of the support.…”

Micropatterning Proteins and Synthetic Peptides on Solid Supports: A Novel Application for Microelectronics Fabrication Technology

“…Immobilization of proteins, glycoproteins, and peptides using cross-linkers is a technique that is employed frequently (Aplix and Hughes, 1981;Bhatia et al, 1989;Burteau et al, 1990). However, our approach, that of patterning immobilized molecules, is novel and of potential importance and applicability in many areas.…”