SSR Markers Associated with Proline in Drought Tolerant Wheat Germplasm

Iqbal, Muhammad; Maqsood, Yasir; Abdin, Zain ul; Manzoor, Atif; Hassan, Muhammad; Jamil, Amer

doi:10.1007/s12010-015-1927-1

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…Further, the cytoplasm undergoes extravasation under drought stress, resulting in an increase in relative conductivity [14]. Under drought stress, proline reduces the osmotic potential, plays a role in osmotic regulation, and protects cell membrane structure; these changes represent an adaptive response of plants to adverse environments [60,61,62]. Our results, together with those of previous studies, strongly suggest that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.…”

Section: Discussionmentioning

confidence: 99%

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Wang

Yan

et al. 2018

IJMS

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Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

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Section: Discussionmentioning

confidence: 99%

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Wang

Yan

et al. 2018

IJMS

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“…A rapid break down of proline upon relief of stress may provide sufficient reducing agents that support mitochondrial oxidative phosphorylation and generation of ATP for recovery from stress and repairing of stress-induced damages [29]. Iqbal et al [30] have reported that the accumulation of proline in drought-tolerant and drought-sensitive cultivars has revealed the significance of this osmolyte. The role of proline in induced PEG experiment gave evidence that the higher levels of proline are due to the emergent need of stressed plant.…”

Section: Prolinementioning

confidence: 99%

“…In plants, trehalose increased the biomass production in shoots and roots in all wheat cul tivars under waterstressed conditions as an osmoprotectant under adverse environmental conditions. Exogenous applications of trehalose and proline to plants during or after stress exposure and the increase in the internal levels of these compounds generally enhance plant growth and final crop yield under stress conditions [30].…”

Section: Trehalosementioning

confidence: 99%

Role of Osmolytes and Antioxidant Enzymes for Drought Tolerance in Wheat

Iqbal¹

2018

Global Wheat Production

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Plants are vital to life as their presence maintains ecosystem on this living globe. Environ mental stresses trigger multiple responses initiated by plant cells to save plant life, from altered gene expression up to changes in cellular metabolism to regulate plant growth rates, which lead to better crop yield. The production of different osmoprotectants like proline, glycine-betaine (GB), trehalose, and antioxidant enzymes like superoxide dis mutase (SOD), catalase (CAT), and ascorbate peroxidase has shown a promising role to keep away cells from immediate cellular damage. Root-to-shoot ratio was enhanced in the drought-affected genotypes, while osmolytes and antioxidant enzymes take up the role to overcome drought situation in wheat germplasm. PEGinduced protocol was used to find out the production of osmolytes (proline, glycine-betaine, and trehalose) and anti oxidant enzymes (SOD, CAT, and APX) biochemically. The levels of antioxidant enzymes and osmolytes were enhanced significantly in all germplasms indicating the defensive measures of plant cells in drought situation. DNA fingerprinting results have shown that the different wheat germplasms have an association with the levels of osmoprotectants and antioxidant enzymes during drought stress.

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“…These markers might be used for marker-assisted selection. Iqbal et al 39 showed that the wheat genotype association with the levels of proline during induced drought stress and the relationship between pattern of drought responsive biochemical attributes and DNA markers in the selected wheat genotypes was recognized to select drought tolerant genotypes. In the same concern, Aly et al 40 demonstrated that peroxidase and polyphenol oxidase activity and gene expressions can be used as biochemical and molecular markers to detect the resistance or susceptibility nature of wheat cultivars against salinity in integration with gamma irradiation.…”

Section: Primer Namementioning

confidence: 99%

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Aly¹,

Eliwa²,

Maraei³

2019

ScienceAsia

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Grains of two bread wheat cultivars (Triticum aestivum), Sids-1 and Sakha-93, were irradiated with gamma rays at dose levels (0.0, 100, 200, and 300 Gy) to study the effects of gamma irradiation on physiological characteristics and genetic variation of wheat. Irradiation dose level of 200 Gy increased chlorophyll a, b, and total chlorophyll contents significantly in both cultivars. Also, proline content increased with increasing irradiation dose level, the highest concentrations were recorded at 300 Gy for Sids-1 and Sakha-93 cultivars as compared to the control. Inter-Simple Sequence Repeat (ISSR) markers have been done to help understanding their genetic differences. Eight ISSR primers (14A, 44B, HB-08, HB-10, HB-11, HB-12, HB-13, and HB-15) exhibited polymorphism with the un-irradiated and irradiated two wheat cultivars. These pimers successfully showed different banding patterns with several amplicons varied from 4 for (14A) to 15 for (HB-10). These 72 amplicons for the two cultivars and an average of 9.0 amplicons with mean of 67.96% polymorphism and resolving power (Rp) of 3.41. It is also cleared that radiation is more effective on Sids-1 cultivar with 55.5% polymorphism than on Sakha-93 cultivar with 51.9% polymorphism. Total amplicons found in Sids-1cultivar were 64, ten of them were unique amplicons (UA): 5 UA(+) and 5 UA(−). Irradiation dose (200 Gy) showed the highest number of UA (3 UA− and 3 UA+) in Sids-1cultivar. While, total amplicons found in Sakha-93 cultivar were 58, eight of them were 5 UA(+) and 3 UA(−). Irradiation dose (300 Gy) showed the highest number of UA as 4 UA+. It could be concluded that gamma irradiation of wheat grains produced an appropriate number of generated variations and that ISSR analysis given a useful molecular marker for the symmetry of the mutants.

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SSR Markers Associated with Proline in Drought Tolerant Wheat Germplasm

Cited by 11 publications

References 12 publications

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

Role of Osmolytes and Antioxidant Enzymes for Drought Tolerance in Wheat

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

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