SSR-based genetic mapping and QTL analysis for timing of spring bud flush, young shoot color, and mature leaf size in tea plant (Camellia sinensis)

Tan, Liqiang; Wang, Liyuan; Xu, Liyi; Wu, Liyun; Peng, Min; Zhang, Cheng‐Cai; Wei, Kang; Bai, Peixian; Li, Hailin; Qi, Gui-Nian

doi:10.1007/s11295-016-1008-9

Cited by 48 publications

(39 citation statements)

References 49 publications

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“…An F 1 population with a clear segregation in BLC (from green to purple) in summer was developed from crossing tea cultivars LJ43 (♀) and Baihaozao (♂) as parents (Tan et al ., ). A moderately saturated SSR‐based genetic linkage map was constructed based on this population, with a major QTL for BLC accounting for 28.1–40.8% of the total phenotype variation identified over 2 years of screening with linkage group 8 (LG8) (Tan et al ., ). Molecular markers of those genes were then used to genotype 170 individuals of the F 1 population (Supporting Information Table S3).…”

Section: Resultsmentioning

confidence: 97%

“…Cultivars of C. sinensis , notably LJ43 and ZJ (purple shoot), and the associated F 1 population resulting from their crossing and segregation in BLC were derived as described (Tan et al ., , ). Tea plants were grown in the experimental tea garden of TRICAAS in Hangzhou, China, and spring leaves and buds were weighed and frozen at −80°C prior to further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…(). Using a genetic linkage map of the F 1 population using LJ43 and BHZ as parents, which had been previously established with 483 SSR markers (Tan et al ., ), both the SSR marker of CsGSTF1 and the dCAPS marker of CsMYB75 were localized on the interval between the SSR marker CsFM1281 and CsFM1932 in LG8 using joinmap 4.0 (Zheng and Tian, ).…”

Section: Methodsmentioning

confidence: 99%

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A coupled role for CsMYB75 and CsGSTF1 in anthocyanin hyperaccumulation in purple tea

et al. 2018

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Cultivars of purple tea (Camellia sinensis) that accumulate anthocyanins in place of catechins are currently attracting global interest in their use as functional health beverages. RNA-seq of normal (LJ43) and purple Zijuan (ZJ) cultivars identified the transcription factor CsMYB75 and phi (F) class glutathione transferase CsGSTF1 as being associated with anthocyanin hyperaccumulation. Both genes mapped as a quantitative trait locus (QTL) to the purple bud leaf color (BLC) trait in F 1 populations, with CsMYB75 promoting the expression of CsGSTF1 in transgenic tobacco (Nicotiana tabacum). Although CsMYB75 elevates the biosynthesis of both catechins and anthocyanins, only anthocyanins accumulate in purple tea, indicating selective downstream regulation. As glutathione transferases in other plants are known to act as transporters (ligandins) of flavonoids, directing them for vacuolar deposition, the role of CsGSTF1 in selective anthocyanin accumulation was investigated. In tea, anthocyanins accumulate in multiple vesicles, with the expression of CsGSTF1 correlated with BLC, but not with catechin content, in diverse germplasm. Complementation of the Arabidopsis tt19-8 mutant, which is unable to express the orthologous ligandin AtGSTF12, restored anthocyanin accumulation, but did not rescue the transparent testa phenotype, confirming that CsGSTF1 did not function in catechin accumulation. Consistent with a ligandin function, transient expression of CsGSTF1 in Nicotiana occurred in the nucleus, cytoplasm and membrane. Furthermore, RNA-Seq of the complemented mutants exposed to 2% sucrose as a stress treatment showed unexpected roles for anthocyanin accumulation in affecting the expression of genes involved in redox responses, phosphate homeostasis and the biogenesis of photosynthetic components, as compared with non-complemented plants.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

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A coupled role for CsMYB75 and CsGSTF1 in anthocyanin hyperaccumulation in purple tea

et al. 2018

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“…A moderately saturated SSR-based genetic linkage map was constructed based on this population (Tan et al, 2013). Further field observation showed that these F1 hybrids had a clear segregation in buds and leaves color in summer tea (Tan et al, 2016). Some kept green, but the other exhibited different degrees of purple color.…”

Section: Plant Materialsmentioning

confidence: 99%

Gene expression analysis of bud and leaf color in tea

Wei

Zhang

et al. 2016

Plant Physiology and Biochemistry

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“…Of these approaches, SSR has become an important technique used in tea plant genetics and breeding for its multi-allelic nature, codominant inheritance, relative abundance, extensive genome coverage and simple detection (Zhao et al, 2008). The SSR markers have been used successfully in genetic diversity and relationship (Fang et al, 2011), the origin of tea gemplasm (Meegahakumbura et al, 2016), the selection core collection (Taniguchi et al, 2014), and the construction of genetic linkage maps in tea plants (Tan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and relationship of Dongting biluochun tea germplasm in Suzhou revealed by SSR markers.

Zhou¹,

Li²,

Hoang³

et al. 2019

PAK.J.BOT.

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Dongting Biluochun (DTBLC) tea is one of the top famous tea in China, which is originated from Suzhou city, Jiangsu Province, China. Generally, DTBLC tea is processed using "Dongting population", which have a great genetic diversity. However, little information has been found so far on its genetic diversity. In order to unveil the relationship among the DTBLC germplasm, 68 tea accessions collected from Suzhou geographical origin growing area were analyzed by using 36 pairs of polymorphic simple sequence repeat (SSR) primers. Finally, a total of 68 tea accessions generated 158 alleles with a mean of 4.39 alleles per locus and 314 genotypes with a mean of 8.72. The polymorphism information content (PIC) was between 0.09 and 0.76, and the mean value was 0.53; the gene diversity index (H) ranged from 0.10 to 0.79, with an average of 0.56, while the average observed heterozygosity (Ho) was 0.50 varied from 0.04 to 0.71. The coefficient of the genetic similarity of SSR makers among the tea accessions ranged from 0.49 to 0.89. These results indicated that a considerable genetic diversity existed among these tea accessions. According to the clustering results, 68 tea accessions were clustered into 5 categories with no obvious geographical distribution characteristics based on the unweighted pair group method of arithmetic mean (UPGMA), which hinted that the territorial division of protection for geographic indication-DTBLC tea was appropriate. However, dendrogram based on Nei's genetic distance revealed complex genetic relationships among DTBLC tea germplasm resources, and these collections possessed impressive level of genetic diversity which could be important for subsequent selective breeding, utilization, and conservation of tea germplasm resources in Jiangsu, China.

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SSR-based genetic mapping and QTL analysis for timing of spring bud flush, young shoot color, and mature leaf size in tea plant (Camellia sinensis)

Cited by 48 publications

References 49 publications

A coupled role for CsMYB75 and CsGSTF1 in anthocyanin hyperaccumulation in purple tea

A coupled role for CsMYB75 and CsGSTF1 in anthocyanin hyperaccumulation in purple tea

Gene expression analysis of bud and leaf color in tea

Genetic diversity and relationship of Dongting biluochun tea germplasm in Suzhou revealed by SSR markers.

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