SSR- Based Genetic Diversity Assessment in Tetraploid and Hexaploid Wheat Populations

Abouzied, Hanaa

doi:10.9734/bbj/2013/4340

Cited by 23 publications

(12 citation statements)

References 51 publications

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“…A similar complementary role of the Anosim test to principal coordinate analysis is reported by Abouzied et al (2013). Morphometric and meristic traits gave Anosim R-values of 0.778 (p<0.0001) and 0.688 (p<0.0001), respectively, indicating significant morphological dissimilarity (or distance) between the regenerants and wild seedlings (Table 7).…”

Section: Analysis Of Similarity (Anosim) and Similarity Percentages (supporting

confidence: 74%

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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Somaclonal variation induced in vitro during tissue culture can be a problem in clonal micropropagation of elite plants. This study investigated the extent of morphological and genetic similarity or dissimilarity between Melia volkensii in vitro plants (somatic seedlings) obtained via somatic embryogenesis and normal seedlings. Comparisons were made between in vitro plants regenerated directly from cotyledon explants, indirectly from zygotic embryos and normal seedlings of the same parent trees. Regeneration was achieved using half MS medium supplemented with 0.05 mg/l thidiazuron. Shoots were elongated in half MS with 0.1 mg/l BAP plus 0.01 mg/l IAA then rooted in half MS with 0.1 mg/l IBA and 0.1 mg/l NAA. Six morphometric and five meristic characters were used for the morphological characterization. PCR-RAPD markers were used for assessment of genetic similarity or distance. Multivariate analysis using principal coordinates, cluster analysis, analysis of similarities (Anosim) and similarity percentages analysis (SIMPER) revealed significant dissimilarities (p< 0.0001) in morphometric and meristic characters between the in vitro plants and normal seedlings. However, significant similarity (p<0.01) was observed in the RAPD-genic characters of the in vitro plants and normal seedlings. Out of six morphometric traits, taproot length, internode length and shoot height were the most important sources of dissimilarity, cumulatively accounting for 72.37% of overall morphometric dissimilarity. Number of lateral roots was the single most important source of meristic dissimilarity, with 77.02% contribution. Plants regenerated directly from cotyledons were more similar to the normal seedlings in morphological and RAPD-marker characters than those regenerated indirectly from zygotic embryos. This study paves the way for identification of trait-specific RAPD markers for further characterization through sequence-characterized amplified regions (SCARs).

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Section: Analysis Of Similarity (Anosim) and Similarity Percentages (supporting

confidence: 74%

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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“…In later years, PAST has grown into a comprehensive statistics package that is used not only by paleontologists, but also in many fields of life science, earth science, and even engineering and economics (Hammer et al, 2001). PAST program was used in DNA fingerprinting (ISSR and RAPD) of Prosopis cineraria and P.juliflora (Elmeer and Almalki, 2011), molecular (SSR) analysis of old apple cultivars (Király et al, 2012), genetic analysis (RAPD) of Hibiscus species (Kadve et al, 2012), molecular (SSR) determination of genetic structure of Brazilian soybean cultivars (Piriolli et al, 2013), SSR-based genetic diversity assessment in tetraploid and hexaploid wheat populations (Abouzied et al, 2013), molecular diversity (SSR) in cultivated groundnut (Goswami et al, 2013). …”

Section: Resultsmentioning

confidence: 99%

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

Polat¹

2015

Derim

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Sweet orange [Citrus sinensis (L.) Osbeck] fruit is one of the main citrus fruits, Navel and Valencia group sweet orange being the most representative and recognizable species of this species. The aims of this study were to determine genetic relationships and diversity of 84 Navel and 36 Valencia groups of sweet orange using SSR (simple sequence repeat) molecular markers. Twenty-six SSR primers were tested on these accessions. Seven SSR primers produced thirteen polymorphic fragments, eight SSR primers produced monomorphic fragments, and eleven SSR primers produced no scorable fragments. Thirteen SSR primers produced a total of 29 fragments and 13 of them were polymorphic. The number of average polymorphic fragments per primer was 1.93. The mean polymorphism information content (PIC) and marker index (MI) are 0.16 and 11.74, respectively. The Dice's similarity coefficient among Navel and Valencia group sweet oranges ranged from 0.42 to 1.00 and matrix correlation (r) was 0.79. In the cluster analysis, Navel group sweet oranges were indicated as a separate group from Valencia group sweet oranges. 'Antalya (40)' was most distinct accessions from the others.

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“…Выявление уникальных и разнообразных аллелей дает возможность исследовать генетическое разнообразие и специфичность сортов, их идентификацию и регистрацию, улучшение культурных растений, включая пшеницу (Abouzied et al, 2013). Кроме того, наличие уникальных аллелей определяет индивидуальность популяций, что подразумевает наличие генетической вариации, необходимой для адаптации к экологическим условиям.…”

Section: Discussionunclassified

“…При изучении генетического разнообразия можно использовать различные подходы, такие как анализ родословных, морфологические признаки или молекулярные маркеры. Использование систем молекулярных маркеров позволяет изучать полиморфизм ДНК, устанавливать генетические взаимоотношения, выявлять гены хозяйственно ценных признаков, что представляет собой актуальное направление в селекции сельскохозяйственных культур (Cox et al, 1985;Motawei et al, 2007;Cifci, Yagdi, 2012;Abouzied et al, 2013;Malik et al, 2013).…”

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Study of the genetic polymorphism of diploid wheat Triticum boeoticum Boiss. using SSR markers

Abbasov¹

2018

Vestn. VOGiS

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SSR- Based Genetic Diversity Assessment in Tetraploid and Hexaploid Wheat Populations

Cited by 23 publications

References 51 publications

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

Study of the genetic polymorphism of diploid wheat Triticum boeoticum Boiss. using SSR markers

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