srv-k Status Aboard the International Space Station During Missions 15 and 16

Spontaneous mutation in the AgrRS two-component regulatory system ofCupriavidus metalliduransresults in enhanced silver resistance

“…Strain NA4 is isolated from potable water from the Russian condensate recycle system SRV-K aboard the ISS. 41,42 In contrast to CH34 where both megaplasmids have a key role in metal detoxification, most of the metal resistance determinants are located on the chromid. 43 For NA4, a spontaneous mutant resistant to silver was selected after incubation in MM284 supplemented with 8 mM AgNO 3 and designated as NA4S.…”

Genome Sequences of Cupriavidus metallidurans Strains NA1, NA4, and NE12, Isolated from Space Equipment

“…In addition, some C. metallidurans strains carry an extensive mobile gene pool ( 5 , 11 , 12 ). C. metallidurans NA1, NA4, and NE12 were isolated from water samples of the potable water system and the condensate water regeneration system of the International Space Station and from air particulates of the Payload Hazardous Servicing Facility located at the Kennedy Space Center in Florida, respectively ( 4 , 13 , 14 ). These isolates were sequenced for comparative analyses and for exploring the adaptation potential to these oligotrophic environments.…”

PrsQ2, a small periplasmic protein involved in increased uranium resistance in the bacterium Cupriavidus metallidurans

“…Recently, we showed that Cupriavidus metallidurans NA4 precipitates uranium from growth medium as uranium phosphate complexes that are associated with polyhydroxybutyrate (Rogiers et al, 2022a). C. metallidurans is a model organism for metal resistance and strains are usually isolated from industrially metal-contaminated sites (Mergeay and Van Houdt, 2015;Bobe et al, 2008). C. metallidurans species contain numerous mobile genetic elements allowing rapid adaptation to toxic circumstances (Mijnendonckx et al, 2011;Van Houdt et al, 2018).…”