srf-3, a Mutant of Caenorhabditis elegans, Resistant to Bacterial Infection and to Biofilm Binding, Is Deficient in Glycoconjugates

Cipollo, John F.; Awad, Antoine; Costello, Catherine E.; Hirschberg, Carlos B.

doi:10.1074/jbc.m409557200

Cited by 63 publications

(63 citation statements)

References 37 publications

(43 reference statements)

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“…Peak B contains a Hex1,6-arm and Peak C contains a Hex1,4-arm. These tetrasaccharide assignments are consistent with the general structural paradigm seen in O-glycans that have been documented in C. elegans [34,49]. The structural assignments are shown to the right of the spectra in Figure 6.…”

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycanssupporting

confidence: 83%

“…elegans O-glycans were released from whole nematode extracts as previously described [34]. Briefly, glycans were released by reductive ␤-elimination in ␤ 0.1M NaOH/1 M NaBH 4 at 60°C for 16 h followed by neutralization with drop-wise addition of glacial acetic acid.…”

Section: Oligosaccharide Release and Reductionmentioning

confidence: 99%

“…The C. elegans O-glycan used here was from a previous study [34] generated from ϳ10-g wet weight of nematodes. LC/MS analyses were performed on ϳ20 ng aliquots of permethylated alditols using the 300 PGC column.…”

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

“…Here the voltage offset C. elegans produces neutral O-glycans that contain a mammalian-like Gal␤1,3GalNAc type I core and are highly branched and short [34,49]. Both core residues can be highly substituted.…”

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

“…Both core residues can be highly substituted. The core Gal of the neutral O-glycans has been reported to be 4,6-disubstituted, while only GlcA has been reported to substitute the C-3 position [34,49]. The core GalNAc can be up to 3,4,6-trisubstituted, whereas the C-3 position has been reported to contain Gal exclusively [49].…”

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

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A glycomics platform for the analysis of permethylated oligosaccharide alditols

Costello

Contado-Miller

Cipollo

2007

J. Am. Soc. Mass Spectrom.

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156

166

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This communication reports the development of an LC/MS platform for the analysis of permethylated oligosaccharide alditols that, for the first time, demonstrates routine online oligosaccharide isomer separation of these compounds before introduction into the mass spectrometer. The method leverages a high-resolution liquid chromatography system with the superior fragmentation pattern characteristics of permethylated oligosaccharide alditols that are dissociated under low-energy collision conditions using quadrupole orthogonal time-offlight (QoTOF) instrumentation and up to pseudo MS 3 mass spectrometry. Glycoforms, including isomers, are readily identified and their structures assigned. The isomer-specific spectra include highly informative cross-ring and elimination fragments, branch position specific signatures, and glycosidic bond fragments, thus facilitating linkage, branch, and sequence assignment. The method is sensitive and can be applied using as little as 40 fmol of derivatized oligosaccharide. Because permethylation renders oligosaccharides nearly chemically equivalent in the mass spectrometer, the method is semiquantitative and, in this regard, is comparable to methods reported using high field NMR and capillary electrophoresis. In this postgenomic age, the importance of glycosylation in biological processes has become clear. The nature of many of the important questions in glycomics is such that sample material is often extremely limited, thus necessitating the development of highly sensitive methods for rigorous structural assignment of the oligosaccharides in complex mixtures. The glycomics platform presented here fulfills these criteria and should lead to more facile glycomics analyses. (J Am

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Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycanssupporting

confidence: 83%

Section: Oligosaccharide Release and Reductionmentioning

confidence: 99%

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

Section: Porous Graphite Lc/ms Of Caenorhabditis Elegans O-glycansmentioning

confidence: 99%

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A glycomics platform for the analysis of permethylated oligosaccharide alditols

Costello

Contado-Miller

Cipollo

2007

J. Am. Soc. Mass Spectrom.

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156

166

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2003–2004

Harvey

2008

Mass Spectrometry Reviews

100

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This review is the third update of the original review, published in 1999, on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings the topic to the end of 2004. Both fundamental studies and applications are covered. The main topics include methodological developments, matrices, fragmentation of carbohydrates and applications to large polymeric carbohydrates from plants, glycans from glycoproteins and those from various glycolipids. Other topics include the use of MALDI MS to study enzymes related to carbohydrate biosynthesis and degradation, its use in industrial processes, particularly biopharmaceuticals and its use to monitor products of chemical synthesis where glycodendrimers and carbohydrate-protein complexes are highlighted.

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8th HUPO World Congress: The Human Disease Glycomics/Proteomics Initiative (HGPI) Session 26 September 2009, Toronto, Canada

Narimatsu

2010

Proteomics

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The Human Disease Glycomics/Proteomics Initiative (HGPI) Session at the HUPO World Congress was held in Toronto on 26 September 2009. In this report, we summarize the presentation of the HGPI workshop as follows: (i) The results of the past HGPI pilot studies (first and second) in which we analyzed N- and O-linked glycans using standard glycoproteins (i.e. first: N-linked glycan analysis of transferrin and IgG; second: O-linked glycan analysis of IgA). (ii) The recent progresses of the current HGPI third pilot study. The third analytical pilot study is in progress to perform the two tasks, which are glycan structural analysis and identification of proteins which carry specific carbohydrate antigen (Lewis X antigen) using cancer cells (i.e. L428, U937, and SK-N-SH), under the theme of "Glyco-Biomarker Discovery". Finally, recently advanced glycomic and glycoproteomic analyses were also reported.

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srf-3, a Mutant of Caenorhabditis elegans, Resistant to Bacterial Infection and to Biofilm Binding, Is Deficient in Glycoconjugates

Abstract: srf-3 is a mutant of C. elegans that is resistant to infection by

Cited by 63 publications

References 37 publications

A glycomics platform for the analysis of permethylated oligosaccharide alditols

A glycomics platform for the analysis of permethylated oligosaccharide alditols

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2003–2004

8th HUPO World Congress: The Human Disease Glycomics/Proteomics Initiative (HGPI) Session 26 September 2009, Toronto, Canada

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