Spurenbestimmung �strogenwirkender Masthilfsmittel in Fleisch durch HPLC mit voltammetrischer Detektion

Frischkorn, C. G. B.; Smyth, Malcolm R.; Frischkorn, H. E.; Golimowski, Jerzy

doi:10.1007/bf01154742

Cited by 28 publications

(2 citation statements)

References 8 publications

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“…2). The irreversible wave, located at high potentials (> 1.2 V), is likely due to oxidation of the OH groups of the steroidal skeleton [24]. The reversible 1e À oxidation process, at E 0 0.51 V vs. SCE, can be easily assigned to the Fc (0/1 ) redox couple.…”

mentioning

confidence: 99%

The Ferrocenylethynyl Unit: a Stable Hormone Tag

Osella¹,

Nervi²,

Galeotti³

et al. 2001

HCA

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We prepared the organometallic complex 17a-(ferrocenylethynyl)estradiol ( [(3,17b-dihydroxyestra-1,3,5(10)-trien-17a-yl)ethynyl]ferrocene; FcEE; 1) by a novel synthetic method. This metallocene possesses sufficient stability in aqueous media to permit the study of its biological properties. Thus, we were able to show that, despite the addition of a bulky substituent at the 17a position of the steroid, the metallocene is still wellrecognized by an antibody specific to estradiol (CR 40%) and by both subtypes (ERa, ERb) of the estrogen receptor (at 08, RBA 28 and 37%, resp.). A DCI-MS study of the stability of the carbocation [FcEE À OH] showed moderate stabilization of the carbocation, in agreement with the pK R value of À 0.72 found for the metallocene by means of Deno×s method. The presence of the ferrocene allows the electrochemical detection of FcEE (1) by HPLC-ED, with a detection limit of ca. 1 nm, suitable for quantitative pharmacological analysis.

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mentioning

confidence: 99%

The Ferrocenylethynyl Unit: a Stable Hormone Tag

Osella¹,

Nervi²,

Galeotti³

et al. 2001

HCA

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“…Phenols exhibit anodic waves at a variety of solid electrodes at relatively positive potentials (÷l.2V -+l.3V) and this can be applied to the analysis of nine estrogenic and pseudo-estrogenic hormones in animal meat (ref. 26) where the use of electrochemical detection permitted the simultaneous determination of eight of these compounds down to pg quantities per g of meat. In addition, this technique was shown to exhibit much less interference from natural constituents in the meat and could selectively determine diethylstilbestrol in the presence of either dienestrol or hexestrol (refs.…”

Section: Unauthenticatedmentioning

confidence: 99%

Electrochemical analysis of organic pollutants

Smyth¹,

Smyth²

1987

Pure and Applied Chemistry

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Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver

Covey

Maylin

Henion

1985

Biol. Mass Spectrom.

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A procedure is described for the extraction of diethylstilbestrol (DES) from animal tissue for quantitative capillary gas chromatography/mass spectrometry (GC/MS). The procedure is based upon use of a strong anion exchange polystyrene divinylbenzene resin for sample purification. The recovery of DES from the resin clean up was 88% in the high parts per trillion (ppt) range. Criteria for identification of DES using selected ion monitoring (SIM) GC/MS are presented. Liquid chromatography/mass spectrometry (LC/MS) was used to investigate altered DES cis/trans ratios observed in biological extracts.

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Spurenbestimmung �strogenwirkender Masthilfsmittel in Fleisch durch HPLC mit voltammetrischer Detektion

Cited by 28 publications

References 8 publications

The Ferrocenylethynyl Unit: a Stable Hormone Tag

The Ferrocenylethynyl Unit: a Stable Hormone Tag

Electrochemical analysis of organic pollutants

Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver

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