Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver

Covey, Thomas R.; Maylin, G. A.; Henion, Jack D.

doi:10.1002/bms.1200120606

Cited by 16 publications

(3 citation statements)

References 23 publications

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“…It must however be emphasized that the ratios, although they might vary from sample extract to sample extract, were comparable within the same analytical run for both (HFB)2-DES and (HFB)2-[2H6]DES. This is consistent with the observations of other authors (Covey et al, 1985). An additional advantage is that the metabolism of the parent drug molecule, which is the trans configuration, is ruled out by the analytical procedure.…”

Section: Resultssupporting

confidence: 92%

Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Peteghem¹,

Lefevere²,

Haver³

et al. 1987

J. Agric. Food Chem.

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Section: Resultssupporting

confidence: 92%

Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Peteghem¹,

Lefevere²,

Haver³

et al. 1987

J. Agric. Food Chem.

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“…Large numbers of samples cannot easily be surveyed by global profiling, which requires the use of a candidate-based approach in which a restricted set of targets are selected for quantitation. Multiple-reaction monitoring (MRM) MS is a multiplexed form of selected ion monitoring that selectively observes an analyte by using two mass filters, first scanning the mass of the parent ion and then transitioning to scanning one or more fragment ions generated by MS/MS . Analytes can be quantified from peak heights of fragment ions, and this has the advantages of achieving greater sensitivity over full-scanning methods because of the restriction in mass range and avoiding interference from coeluting molecules in complex mixtures.…”

Section: Quantifying Changes In Protein Abundancementioning

confidence: 99%

Achieving In-Depth Proteomics Profiling by Mass Spectrometry

Ahn

Shabb

Old³

et al. 2007

ACS Chem. Biol.

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Proteomics addresses the important goal of determining the chemistry and composition of proteins in biological samples. Mass-spectrometry-based strategies have been highly successful in identifying and profiling proteins in complex mixtures; however, although depth of sampling continues to improve, a general recognition exists that no study has yet achieved complete protein coverage in any tissue, cell type, subcellular component, or fluid. The development of new approaches for comprehensively surveying highly complex protein mixtures, distinguishing protein isoforms, quantifying changes in protein abundance between different samples, and mapping post-translational modifications are areas of active research. These will be needed to achieve the "systems-wide" protein profiling goals of defining molecular responses to cell perturbations and obtaining biomarker information for disease detection, prognosis, and responses to therapy. We review recent progress in approaching these problems and present examples of successful applications and the outlook for the future.

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“…It is generally accepted that confirmation under selected ion monitoring (SIM) GC/MS conditions requires that one monitor at least two or preferably three noncontiguous ions and that their relative abundances be within ±10−20% of the absolute relative abundance obtained for a reference standard of the compound analyzed under the same conditions . In addition, the GC retention time should be within a certain percentage of a reference standard of that compound analyzed under the same experimental conditions …”

Section: Resultsmentioning

confidence: 99%

Acceptance Criteria for Ultratrace HPLC−Tandem Mass Spectrometry: Quantitative and Qualitative Determination of Sulfonylurea Herbicides in Soil

et al. 1996

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Eight commonly used sulfonylureas (SUs: nicosulfuron, thifensulfuron methyl, metsulfuron methyl, sulfometuron methyl, chlorsulfuron, bensulfuron methyl, tribenuron methyl, and chlorimuron methyl) and deuterium-labeled nicosulfuron (nicosulfuron-d(6)), used as an internal standard, were isolated from soil by solvent extraction and identified under quantitative and qualitative ion spray LC/MS/MS conditions using the selected reaction monitoring (SRM) mode of acquisition. The lower level of quantitation for these SUs in soil was determined at the 0.05 ppb level using a TurboIonSpray adapted LC/MS interface without a precolumn split and optimizing MS/MS tuning conditions for individual SUs. The eight SUs were qualitatively identified and quantitatively determined in soil. The standard curve for each SU was linear from 0.05 to 10 ppb. This SRM LC/MS method demonstrates high sensitivity and high specificity for these SUs in soil and shows at least a 400-fold improvement in sensitivity over previous reports. Acceptance criteria for forensically valid data are suggested for qualitative SRM LC/MS experiments. These include HPLC retention time reproducibility (±2%), at least two and preferably three precursor-product ions selected, and relative abundance criteria for selected ions (±20% absolute).

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Quantitative secondary ion monitoring gas chromatography/mass spectrometry of diethylstilbestrol in bovine liver

Cited by 16 publications

References 23 publications

Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Achieving In-Depth Proteomics Profiling by Mass Spectrometry

Acceptance Criteria for Ultratrace HPLC−Tandem Mass Spectrometry: Quantitative and Qualitative Determination of Sulfonylurea Herbicides in Soil

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