Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems

Miranda, Catarina S.; Ribeiro, Ana R. M.; Homem, Natália C.; Felgueiras, Helena P.

doi:10.3390/antibiotics9040174

Cited by 30 publications

(30 citation statements)

References 231 publications

(273 reference statements)

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“…Hence, the interest in the research for novel alternatives to antibiotics has been growing. Natural products are becoming very promising as antimicrobial agents, being considered safe and environmentally friendly [9,10]. Antimicrobial peptides (AMPs) of natural origin have been the focus of great interest as alternatives to conventional antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Activity of Specialized Biomolecules against Gram-Positive and Gram-Negative Bacteria

et al. 2020

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The increased resistance of bacteria against conventional pharmaceutical solutions, the antibiotics, has raised serious health concerns. This has stimulated interest in the development of bio-based therapeutics with limited resistance, namely, essential oils (EOs) or antimicrobial peptides (AMPs). This study envisaged the evaluation of the antimicrobial efficacy of selected biomolecules, namely LL37, pexiganan, tea tree oil (TTO), cinnamon leaf oil (CLO) and niaouli oil (NO), against four bacteria commonly associated to nosocomial infections: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. The antibiotic vancomycin and silver nanoparticles (AgNPs) were used as control compounds for comparison purposes. The biomolecules were initially screened for their antibacterial efficacy using the agar-diffusion test, followed by the determination of minimal inhibitory concentrations (MICs), kill-time kinetics and the evaluation of the cell morphology upon 24 h exposure. All agents were effective against the selected bacteria. Interestingly, the AgNPs required a higher concentration (4000–1250 μg/mL) to induce the same effects as the AMPs (500–7.8 μg/mL) or EOs (365.2–19.7 μg/mL). Pexiganan and CLO were the most effective biomolecules, requiring lower concentrations to kill both Gram-positive and Gram-negative bacteria (62.5–7.8 μg/mL and 39.3–19.7 μg/mL, respectively), within a short period of time (averaging 2 h 15 min for all bacteria). Most biomolecules apparently disrupted the bacteria membrane stability due to the observed cell morphology deformation and by effecting on the intracellular space. AMPs were observed to induce morphological deformations and cellular content release, while EOs were seen to split and completely envelope bacteria. Data unraveled more of the potential of these new biomolecules as replacements for the conventional antibiotics and allowed us to take a step forward in the understanding of their mechanisms of action against infection-related bacteria.

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Section: Introductionmentioning

confidence: 99%

Activity of Specialized Biomolecules against Gram-Positive and Gram-Negative Bacteria

et al. 2020

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“…Still their cytotoxicity at increased concentrations, their low resistance to degradation by external factors (e.g., temperature, light, moisture), and their volatility in their free, liquid form remain difficult challenges to overcome for their expanded use. Here, fibers and fibrous constructs have emerged as optional platforms for their targeted and therapeutic delivery, this way promoting their application in the combat against drug-resistant bacteria [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…The wet-spinning technique is a nonsolvent induced phase inversion approach that allows the production of continuous polymeric microfibers, with uniform morphology, by injecting a polymer solution into a nonsolvent coagulation bath that prompts the solidification of the extruded material [7,9]. This technique has been raising attention in the biomedical field for its simple set-up, enabling the production of fibers at the microscale for controlled drug release and/or tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Physical, Thermal, and Antibacterial Effects of Active Essential Oils with Potential for Biomedical Applications Loaded onto Cellulose Acetate/Polycaprolactone Wet-Spun Microfibers

et al. 2020

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New approaches to deal with the growing concern associated with antibiotic-resistant bacteria are emerging daily. Essential oils (EOs) are natural antimicrobial substances with great potential to mitigate this situation. However, their volatile nature, in their liquid-free form, has restricted their generalized application in biomedicine. Here, we propose the use of cellulose acetate (CA)/polycaprolactone (PCL) wet-spun fibers as potential delivery platforms of selected EOs to fight infections caused by Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Twenty EOs were selected and screened for their minimal inhibitory concentration (MIC), using the antibiotic ampicillin as positive control. The cinnamon leaf oil (CLO), cajeput oil (CJO), and the clove oil (CO) were the most effective EOs, against the Gram-positive (MIC < 22.38 mg/mL) and the Gram-negative (MIC < 11.19 mg/mL) bacteria. Uniform microfibers were successfully wet-spun from CA/PCL with an averaged diameter of 53.9 ± 4.5 µm, and then modified by immersion with CLO, CJO and CO at 2 × MIC value. EOs incorporation was confirmed by UV-visible spectroscopy, Fourier-transformed infrared spectroscopy, and thermal gravimetric analysis. However, while microfibers contained ampicillin at MIC (control) after the 72 h modification, the CLO, CO and CJO-loaded fibers registered ≈ 14%, 66%, and 76% of their MIC value, respectively. Data showed that even at small amounts the EO-modified microfibers were effective against the tested bacteria, both by killing bacteria more quickly or by disrupting more easily their cytoplasmic membrane than ampicillin. Considering the amount immobilized, CLO-modified fibers were deemed the most effective from the EOs group. These results indicate that CA/PCL microfibers loaded with EOs can be easily produced with increased antibacterial action, envisioning their use as scaffolding materials for the treatment of infections.

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“…Regarding their chemical properties, they have polyfunctionality, high chemical reactivity, chirality, chelation and adsorption capacity, which allow them to be chemically and biochemically modified very easily. These modifications result in different polysaccharide derivatives, which increase the range of applications [ 6 , 8 , 11 ]. The alginate, hyaluronic acid (HA), cellulose and chitosan (CS) stand out between the polysaccharides for being the most used in biomedicine ( Table 1 ).…”

Section: Polymers Natural/syntheticmentioning

confidence: 99%

Recent Advances in Fiber–Hydrogel Composites for Wound Healing and Drug Delivery Systems

2021

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In the last decades, much research has been done to fasten wound healing and target-direct drug delivery. Hydrogel-based scaffolds have been a recurrent solution in both cases, with some reaching already the market, even though their mechanical stability remains a challenge. To overcome this limitation, reinforcement of hydrogels with fibers has been explored. The structural resemblance of fiber–hydrogel composites to natural tissues has been a driving force for the optimization and exploration of these systems in biomedicine. Indeed, the combination of hydrogel-forming techniques and fiber spinning approaches has been crucial in the development of scaffolding systems with improved mechanical strength and medicinal properties. In this review, a comprehensive overview of the recently developed fiber–hydrogel composite strategies for wound healing and drug delivery is provided. The methodologies employed in fiber and hydrogel formation are also highlighted, together with the most compatible polymer combinations, as well as drug incorporation approaches creating stimuli-sensitive and triggered drug release towards an enhanced host response.

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Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems

Cited by 30 publications

References 231 publications

Activity of Specialized Biomolecules against Gram-Positive and Gram-Negative Bacteria

Activity of Specialized Biomolecules against Gram-Positive and Gram-Negative Bacteria

Physical, Thermal, and Antibacterial Effects of Active Essential Oils with Potential for Biomedical Applications Loaded onto Cellulose Acetate/Polycaprolactone Wet-Spun Microfibers

Recent Advances in Fiber–Hydrogel Composites for Wound Healing and Drug Delivery Systems

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