Sprout results characterization of in vitro shoots encapsulation broccoli using sodium alginate

Abstract

One of the in vitro culture techniques to obtain planting material in vitro is through artificial seed technology. The purpose of this research was to obtain a description and test the effects of sodium alginate on in vitro shoot encapsulation in broccoli. Research result for 7 days of storage the highest germination rate of 10 percent in treatment S1 (2 milligram per litre 6- Furfuryl amino purine (KIN)) which was encapsulated with 2 percent sodium alginate (E1). During the 28 days storage the germination rate increased significantly to 30 percent in the S2 treatment (1 milligram per litre KIN plus 0.5 milligram per litre naphthalene acetic acid (NAA)) and S3 (2 milligram per litre KIN plus 0.5 milligram per litre NAA) which was also encapsulated with E1. The percentage of synthetic seed germination rate correlated closely and positively with the number of seeds form sprouts (r is equal to 0.87). Based on the description data and selection results number of seeds germinating, two encapsulation treatments were selected, that is treatment S2 and S3 encapsulated with E1, which has a sprout stem shape round is not straight. The selected encapsulation treatment can be used for synthetic seed formation by coating.

Production of Synthetic Seeds in Vegetable Crops: A Review

“…It was concluded that in vitro encapsulation seeds are the product of in vitro shoot encapsulation of broccoli with sodium alginate that germinate effectively through alginate capsules. The seed germination rate increases significantly after storage for up to 28 days, notably with the 2% Na-alginate encapsulation treatment, as compared to other encapsulation treatments [60].…”