Spreading depolarization waves triggered by vagal stimulation in the embryonic chick brain: optical evidence for intercellular communication in the developing central nervous system

Momose‐Sato, Yoko; Sato, Katsushige; Mochida, Hiraku; Yazawa, Itaru; Sasaki, Shinichi; Kamino, Kohtaro

doi:10.1016/s0306-4522(00)00477-2

Cited by 26 publications

(42 citation statements)

References 58 publications

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“…The only comparable phenomenon noted in past studies is the depolarization wave in the chick embryo, which was also recorded using the optical recording technique with voltage-sensitive Characteristics of the wave activity in the rat embryo (E16) compared with those of the depolarization wave in the E8 chick embryo. Data for the chick embryo are from Momose- Sato et al (2001bSato et al ( , 2003a and Mochida et al (2001) except for the conduction velocity which was re-evaluated using the method applied to the rat embryo. -, no significant optical signal was detected (⌬I/I Ͻ 2.0 ϫ 10 Ϫ4 ); ϩ, ⌬I/I Ն 2.0 ϫ 10…”

Section: Discussionmentioning

confidence: 99%

“…‡, the specificity of the blockers has not been established. dyes (Mochida et al 2001;Momose-Sato et al 2001b, 2003a. Between the wave activity in the rat embryo and the depolarization wave in the chick embryo, there are many similarities (summarized in Table 5).…”

Section: ϫ4mentioning

confidence: 99%

“…In some experiments, we used artificial cerebrospinal fluid (ACSF) that contained (in mM) 124 NaCl, 5 KCl, 1 MgSO 4 , 2.5 CaCl 2 , 1.25 NaH 2 PO 4 , 22 NaHCO 3 , and 10 glucose, equilibrated with a mixture of 95% O 2 -5% CO 2 (pH 7.4) and obtained similar results. The pia mater was carefully removed in the bathing solution under a dissection microscope, and the preparation was next stained by incubating it for 10 -20 min in the solution containing 0.2-0.4 mg/ml of the voltage-sensitive merocyanine-rhodanine dye NK2761 (Hayashibara Biochemical Laboratories/Kankoh-Shikiso Kenkyusho, Okayama, Japan) (Momose- Sato et al 1995). After staining, the preparation was attached to the silicone bottom of a recording chamber with the ventral or dorsal side facing up by pinning it down with tungsten wires.…”

Section: Preparationsmentioning

confidence: 99%

“…Optical signals were not detected at 630 nm (Fig. 1C), where the membrane potential-related absorption change of NK2761 is absent (Momose- Sato et al 1995) artifacts. Similar results were obtained from 24 brain stem preparations dissected from E15 to E16 embryos, although the responses in the cerebellum were significant (⌬I/I Ն 2.0 ϫ 10 Ϫ4 ) only in five E16 preparations (Tables 1 and 2).…”

Section: Widely Propagating Depolarizing Wave Activity In the Embryonmentioning

confidence: 99%

“…This activity was recorded using a multiple-site optical recording technique with a fast voltage-sensitive dye as widely spreading depolarizing optical signals and was termed the depolarization wave (Momose- Sato et al 2001b). One novel phenomenon of the depolarization wave is that it travels over a wide region of the CNS including the medulla, spinal cord, pons, cerebellum, midbrain, and telencephalon (Mochida et al 2001;Momose-Sato et al 2001b, 2003a. Another outstanding feature is that it is nonspecifically triggered by various types of cranial and spinal sensory inputs as well as endogenous spontaneous activity (Mochida et al 2001;Momose-Sato et al 2003b).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Optical Imaging of Large-Scale Correlated Wave Activity in the Developing Rat CNS

Momose‐Sato

Honda²,

Sasaki³

et al. 2005

Journal of Neurophysiology

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. Correlated neuronal activity plays a fundamental role in the development of the nervous system. Using a multiple-site optical recording technique with a fast voltage-sensitive dye, we previously reported a novel form of correlated activity in the chick embryo, which showed wide propagation throughout the CNS. In this study, we report that similar wave activity is generated in the embryonic rat CNS. Electrical stimulation applied to the cervical cord evoked wave activity that traveled over a wide region of the CNS including the medulla, pons, midbrain, diencephalon, and spinal cord. Small signals were also detected from the cerebellum and part of the cerebrum. Stimulation applied to the cranial nerves such as the trigeminal and vagus nerves evoked waves with similar patterns, indicating that the wave is triggered by external sensory inputs. This wave activity was inhibited by glutamate-, acetylcholine-, GABA-and glycine-receptor antagonists in addition to gap junction blockers such as octanol and 18␤-glycyrrhetinic acid. In the immunohistochemical study, significant immunoreactivity of connexin26 and connexin32 was also observed. Wave activity detected with a voltage-sensitive dye was accompanied by a Ca 2ϩ -wave, indicating that it not only provides electrical synchrony but also biochemical signals associated with [Ca 2ϩ ] i elevation. These characteristics of the wave activity are similar to those of the depolarization wave reported in the chick embryo, suggesting that the large-scale depolarization wave is globally generated across different species.

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Section: Discussionmentioning

confidence: 99%

Section: ϫ4mentioning

confidence: 99%

Section: Preparationsmentioning

confidence: 99%

Section: Widely Propagating Depolarizing Wave Activity In the Embryonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optical Imaging of Large-Scale Correlated Wave Activity in the Developing Rat CNS

Momose‐Sato

Honda²,

Sasaki³

et al. 2005

Journal of Neurophysiology

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Monitoring Population Membrane Potential Signals During Functional Development of Neuronal Circuits in Vertebrate Embryos

Momose‐Sato

Sato

Kamino

2010

Membrane Potential Imaging in the Nervous System

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Monitoring Population Membrane Potential Signals During Development of the Vertebrate Nervous System

Momose‐Sato

Sato

Kamino

2015

Advances in Experimental Medicine and Biology

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The functional organization of the vertebrate central nervous system (CNS) during the early phase of development has long been unclear, because conventional electrophysiological means have several technical limitations. First, early embryonic neurons are small and fragile, and the application of microelectrodes is often difficult. Second, the simultaneous recording of electrical activity from multiple sites is limited, and as a consequence, response patterns of neural networks cannot be assessed. Optical recording techniques with voltage-sensitive dyes have overcome these obstacles and provided a new approach to the analysis of the functional development/organization of the CNS. In this review, we provide detailed information concerning the recording of optical signals in the embryonic nervous system. After outlining methodological considerations, we present examples of recent progress in optical studies on the embryonic nervous system with special emphasis on two topics. The first is the study of how synapse networks form in specific neuronal circuits. The second is the study of non-specific correlated wave activity, which is considered to play a fundamental role in neural development. These studies clearly demonstrate the utility of fast voltage-sensitive dye imaging as a powerful tool for elucidating the functional organization of the vertebrate embryonic CNS.

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Spreading depolarization waves triggered by vagal stimulation in the embryonic chick brain: optical evidence for intercellular communication in the developing central nervous system

Cited by 26 publications

References 58 publications

Optical Imaging of Large-Scale Correlated Wave Activity in the Developing Rat CNS

Optical Imaging of Large-Scale Correlated Wave Activity in the Developing Rat CNS

Monitoring Population Membrane Potential Signals During Functional Development of Neuronal Circuits in Vertebrate Embryos

Monitoring Population Membrane Potential Signals During Development of the Vertebrate Nervous System

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