Spreading Advantages of Coresident Plasmids
 bla
 CTX-M
 -Bearing IncFII and
 mcr-1
 -Bearing IncI2 in Escherichia coli

He, Kun; Li, Wenya; Zhao, Bing; Xu, Hui; Pan, Yongchu; He, Dongjian; Hu, Gongzheng; Wu, Hua; Li, Yuan

doi:10.1128/spectrum.01706-21

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…Thus, through horizontal transmission, the isolates could spread multi-to pandrug resistance to other organisms, thereby jeopardizing antimicrobial therapy. Notably, mcr-1 and the carbapenem determinant bla NDM-4 were in the IncHI2/ST3-type plasmid of a chicken E. coli transconjugant [159], suggesting that coresident plasmids containing mcr and another resistance gene are more stable, confer more of a fitness advantage, and are easier to transfer and cotransfer than a single plasmid [29]. Therefore, coresident mcr plasmids could seriously threaten clinical therapy and public health.…”

Section: Eastern Asia Chinamentioning

confidence: 99%

“…The cocarriage of mcr with plasmid-encoded genes conferring resistance to fluoroquinolones and other last-resort HP-CIAs, such as genes encoding flavin monooxygenenases (TetX) and resistance-nodulation-drug efflux pumps (TmexCD-toprJ) (which mediate resistance to tigecycline), carbapenemases (which mediate resistance to carbapenems), extended-spectrum β-lactamases (ESBLs) and plasmidic ampicillinase C (pAmpC) (which mediate resistance to extended-spectrum cephalosporins), and glutathione S transferase (which mediate resistance to fosfomycin) is of further concern because such strains could become XDR or pandrug-resistant (PDR) in a single event of HGT, which could result in nearly untreatable infections, fatalities, and huge economic losses [26]. Unfortunately, plasmids carrying mcr genes with other last-resort antimicrobial determinants seem to be more stable, giving carriers a greater fitness advantage and enabling the easier and more rapid transfer of genes [29].…”

Section: Introductionmentioning

confidence: 99%

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Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

et al. 2023

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Mobile colistin resistance (mcr) genes (mcr-1 to mcr-10) are plasmid-encoded genes that threaten the clinical utility of colistin (COL), one of the highest-priority critically important antibiotics (HP-CIAs) used to treat infections caused by multidrug-resistant and extensively drug-resistant bacteria in humans and animals. For more than six decades, COL has been used largely unregulated in the poultry sector in low- and middle-income countries (LMICs), and this has led to the development/spread of mcr gene-containing bacteria (MGCB). The prevalence rates of mcr-positive organisms from the poultry sector in LMICs between January 1970 and May 2023 range between 0.51% and 58.8%. Through horizontal gene transfer, conjugative plasmids possessing insertion sequences (ISs) (especially ISApl1), transposons (predominantly Tn6330), and integrons have enhanced the spread of mcr-1, mcr-2, mcr-3, mcr-4, mcr-5, mcr-7, mcr-8, mcr-9, and mcr-10 in the poultry sector in LMICs. These genes are harboured by Escherichia, Klebsiella, Proteus, Salmonella, Cronobacter, Citrobacter, Enterobacter, Shigella, Providencia, Aeromonas, Raoultella, Pseudomonas, and Acinetobacter species, belonging to diverse clones. The mcr-1, mcr-3, and mcr-10 genes have also been integrated into the chromosomes of these bacteria and are mobilizable by ISs and integrative conjugative elements. These bacteria often coexpress mcr with virulence genes and other genes conferring resistance to HP-CIAs, such as extended-spectrum cephalosporins, carbapenems, fosfomycin, fluoroquinolone, and tigecycline. The transmission routes and dynamics of MGCB from the poultry sector in LMICs within the One Health triad include contact with poultry birds, feed/drinking water, manure, poultry farmers and their farm workwear, farming equipment, the consumption and sale of contaminated poultry meat/egg and associated products, etc. The use of pre/probiotics and other non-antimicrobial alternatives in the raising of birds, the judicious use of non-critically important antibiotics for therapy, the banning of nontherapeutic COL use, improved vaccination, biosecurity, hand hygiene and sanitization, the development of rapid diagnostic test kits, and the intensified surveillance of mcr genes, among others, could effectively control the spread of MGCB from the poultry sector in LMICs.

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Section: Eastern Asia Chinamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

et al. 2023

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Transfer dynamics of antimicrobial resistance among gram-negative bacteria

Wang,

Farhan,

Yuan

et al. 2024

Science of The Total Environment

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Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla _CTX-M -bearing IncFII plasmid in Escherichia coli

He,

Lin,

Liang

et al. 2024

Microbiol Spectr

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The interaction between coexisting plasmids can affect plasmid-carried resistance gene persistence and spread. However, whether the persistence of the bla CTX-M gene in clinical Enterobacteriaceae is related to the interaction of coresident nonresistance-conferring plasmids has not been reported. This study was initiated to elucidate how a nonresistance-conferring IncI1 plasmid affected the bla CTX-M -bearing IncFII plasmid colocated on the same cell. Herein, we constructed three isogenic derivatives of E. coli C600, designated as C600 FII , C600 I1 , and C600 FII+I1 , which harbored the bla CTX-M -IncFII plasmid and/or the nonresistance-IncI1 one. We discovered that strain C600 FII+I1 conferred higher fitness advantages than strain C600 FII ; also, the stability of the bla CTX-M -IncFII plasmid was noticeably improved in an antibiotic-free environment when it coexisted with the IncI1 plasmid. To further explore why the IncI1 plasmid enhanced the persistence of the bla CTX-M -IncFII plasmid, we assessed the bla CTX-M -IncFII plasmid's copy numbers, conjugation frequencies, and rep gene expressions in strains C600 FII and C600 FII+I1 . The results demonstrated that the rep expressions of the bla CTX-M -IncFII plasmid in strain C600 FII+I1 was greatly decreased, along with the plasmid’s copy numbers and mating efficiencies, compared to those in strain C600 FII . Moreover, further study revealed that the intracellular ATP levels of strain C600 FII+I1 were far lower than those of strain C600 FII . Our findings confirmed that coexistence of the nonresistance-IncI1 plasmid can keep the bla CTX-M -IncFII plasmid more stable by increasing the fitness advantages of the host bacteria, which will pose a threat to preventing the long-term presence of the plasmid-carried bla CTX-M gene in clinical Enterobacteriaceae. IMPORTANCE So far, plasmid-carried bla CTX-M is still the most common extended-spectrum beta-lactamase (ESBL) genotype in clinical settings worldwide. Except for the widespread use of third-generation cephalosporins, the interaction between coexisting plasmids can also affect the long-term stable existence of the bla CTX-M gene; however, the study on that is still sparse. In the present study, we assess the interaction of coinhabitant plasmids bla CTX-M -IncFII and nonresistance-IncI1. Our results confirmed that the increased fitness advantages of strain C600 FII+I1 were attributable to the cohabitant nonresistance-IncI1 plasmid, which largely reduced the intracellular ATP levels of host bacteria, thus decreasing the rep gene expression of the bla CTX-M -IncFII plasmid, its copy numbers, and mating efficiencies, while the higher fitness advantages of strain C600 FII+I1 enhanced the persistence of the bla CTX-M -IncFII plasmid. The results indicate that the nonresistance-IncI1 plasmid contributes to the long-term existence of the bla CTX-M -IncFII plasmid, implying a potentially new strategy for controlling the spread of resistance plasmids in clinical settings by targeting nonresistance plasmids.

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Spreading Advantages of Coresident Plasmids bla _CTX-M -Bearing IncFII and mcr-1 -Bearing IncI2 in Escherichia coli

Abstract: More and more Enterobacteriaceae carry both bla CTX-M and mcr-1 , which are usually located on IncFII-type and IncI2-type plasmids in the same bacterial host, respectively. However, the study on advantages of coresident plasmids in bacterial host is still sparse.

Cited by 4 publications

References 28 publications

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Transfer dynamics of antimicrobial resistance among gram-negative bacteria

Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla _CTX-M -bearing IncFII plasmid in Escherichia coli

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Spreading Advantages of Coresident Plasmids bla CTX-M -Bearing IncFII and mcr-1 -Bearing IncI2 in Escherichia coli

Abstract: More and more Enterobacteriaceae carry both bla CTX-M and mcr-1 , which are usually located on IncFII-type and IncI2-type plasmids in the same bacterial host, respectively. However, the study on advantages of coresident plasmids in bacterial host is still sparse.

Cited by 4 publications

References 28 publications

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies

Transfer dynamics of antimicrobial resistance among gram-negative bacteria

Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla CTX-M -bearing IncFII plasmid in Escherichia coli

Contact Info

Product

Resources

About

Spreading Advantages of Coresident Plasmids bla _CTX-M -Bearing IncFII and mcr-1 -Bearing IncI2 in Escherichia coli

Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla _CTX-M -bearing IncFII plasmid in Escherichia coli