Spread of Antibiotic and Antimicrobial Susceptibility of ESBL-Producing &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated from Wild and Cultured Fish in the Mekong Delta, Vietnam

Hon, Nguyen Thi Ngoc; Hoa, Trần Thị Tuyết; Thịnh, Nguyễn Quốc; Hinenoya, Atsushi; Nakayama, Tadasi; Harada, Kaoru; Asayama, Megumi; Warisaya, Minae; Hirata, Kazumasa; Phương, Nguyễn Thanh; Yamamoto, Yoshimasa

doi:10.3147/jsfp.51.s75

Cited by 21 publications

(12 citation statements)

References 18 publications

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“…Sensitivity to ciprofloxacin and gentamycin was reported in an African study [ 25 ], as in this study. In contrast, studies from India (ciprofloxacin; 60.5%) [ 22 ] and Vietnam (ciprofloxacin; 78.6% and gentamycin; 88.3%) [ 27 ] documented higher levels of resistance to the aforementioned antibiotics. Studies from Vietnam and India also consistently reported higher resistance against several other antibiotics tested in this study.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Escherichia coli, Salmonella and Vibrio Derived from Farm-Raised Red Hybrid Tilapia (Oreochromis spp.) and Asian Sea Bass (Lates calcarifer, Bloch 1970) on the West Coast of Peninsular Malaysia

et al. 2022

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Antibiotics are widely used in intensive fish farming, which in turn increases the emergence of antimicrobial-resistant (AMR) bacteria in the aquatic environment. The current study investigates the prevalence and determines the antimicrobial susceptibility of E. coli, Salmonella, and Vibrio in farmed fishes on the west coast of Peninsular Malaysia. Over a period of 12 months, 32 aquaculture farms from the Malaysian states of Selangor, Negeri Sembilan, Melaka, and Perak were sampled. Both E. coli and Salmonella were highly resistant to erythromycin, ampicillin, tetracycline, and trimethoprim, while Vibrio was highly resistant to ampicillin and streptomycin. Resistance to the antibiotics listed as the highest priority and critically important for human therapy, such as colistin in E. coli (18.1%) and Salmonella (20%) in fish, is a growing public health concern. The multi-drug resistance (MDR) levels of E. coli and Salmonella in tilapia were 46.5% and 77.8%, respectively. Meanwhile, the MDR levels of E. coli, Salmonella, V. parahaemolyticus, V. vulnificus and V. cholerae in Asian seabass were 34%, 100%, 21.6%, 8.3% and 16.7%, respectively. Our findings provide much-needed information on AMR in aquaculture settings that can be used to tailor better strategies for the use of antibiotics in aquaculture production at the local and regional levels.

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Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Escherichia coli, Salmonella and Vibrio Derived from Farm-Raised Red Hybrid Tilapia (Oreochromis spp.) and Asian Sea Bass (Lates calcarifer, Bloch 1970) on the West Coast of Peninsular Malaysia

et al. 2022

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“…Our findings agree with previous work showing the occurrence of ESBL- Ec in fishes from Swiss lakes (Abgottspon et al, 2014; Zurfluh et al, 2015). Several studies have already shown the presence of ESBL- Ec in fish from farmed (Jiang et al, 2012) or wild population in Asia (Hon et al, 2016) and in Africa (Moremi et al, 2016). Although E. coli does not seem to be a permanent inhabitant of fish guts (Austin, 2006), these bacteria are able to colonize the fish gut, especially in omnivorous fishes that, at least partially, feed from contaminated biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, fishes are mobile, have different feeding habits and use various microhabitats in the river (Popova and Sytina, 1977; Hyslop, 1982; Weatherley, 1987; Garner, 1996), thus freshwater fishes might also disseminate ESBL- Ec through their movements and might serve as integrators of a freshwater ecosystems bacterial contamination. Indeed, recently several authors have documented the contamination of wild fish in marine environments (Brahmi et al, 2018) and in lakes (Abgottspon et al, 2014; Zurfluh et al, 2015; Moremi et al, 2016) as well as in farmed fish (Jiang et al, 2012; Hon et al, 2016), stressing that fishes could participate in the spread of multidrug resistant enterobacteria. However, such data is missing for fishes from rivers and streams.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of CTX-M-Producing Escherichia coli in Freshwater Fishes From a French Watershed (Burgundy)

et al. 2019

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The burden of extended-spectrum β-lactamases producing Escherichia coli (ESBL-Ec), has increased over several decades. Freshwater ecosystems are suspected to play an important ecological and evolutionary role in driving the dissemination of antimicrobial resistance. The aim of our study was to decipher the occurrence of ESBL-Ec in a small watershed (Ouche river, Burgundy, France), targeting environmental matrices and fishes. Among cefotaxime resistant E. coli (ctxR Ec) isolates, we detected and characterized 36 ESBL-Ec from water, biofilm and fish guts. ctxR Ec and ESBL-Ec were found in samples from sites near the first small town, located downstream from the watershed which was studied. Treatment of urban wastewater by waste water treatment plants (WWTP), might therefore be a major potential source of ctxR Ec and thus of ESBL-Ec. Prevalence of total E. coli and ctxR Ec in fish guts ranged between 0 to 92% and 0 to 85%; respectively, depending on the sampling site and the fish species. The diet of fish (predator or omnivore) seems to strongly influence the prevalence of total E. coli and ESBL-Ec. Extended spectrum beta-lactamases produced by the isolates from this study belonged to the CTX-M family (CTX-M group 1 and 9). Moreover, some environmental ESBL-Ec proved to share genotypic features (MLST types) with isolates which originated from 8 WWTP effluents discharged in the Ouche river and with the sequence type ST131, which is widely described in clinical isolates. Ninety-seven % (97%) of ESBL-Ec from the study harbored additional antibiotic resistances and can thus be considered as multi drug resistant (MDR) bacteria. Finally, 53% of the ESBL-Ec strains harbored class 1 integron-integrase (intl1). These results are discussed with the perspective of defining indicators of antibiotic resistance contamination in freshwater ecosystems.

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“…Therefore, the prevalence of ESBLproducing E. coli was also investigated for 16 wild fish and 32 cultured fish (16 each of Pangasianodon hypophthalmus and Oreochromis spp.). Hon et al (2016) isolated ESBL-producing E. coli from 44.4 and 36.1% of wild (8/18) and cultured (13/ 36) fish, respectively. In total, 42 ESBL-producing E. coli were isolated from 54 fish and most of them were multi-drug resistant.…”

Section: Prevalence Of Esbl-producing Bacteria In Livestock and Fish mentioning

confidence: 99%

Prevalence of extended-spectrum β-lactamase-producing Escherichia coli and residual antimicrobials in the environment in Vietnam

Yamasaki

Vien

et al. 2017

Anim. Health. Res. Rev.

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Emergence and spread of antimicrobial-resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, have become serious problems worldwide. Recent studies conducted in Vietnam revealed that ESBL-producing E. coli are widely distributed in food animals and people. CTX-M-9 and CTX-M-1 are the most prevalent β-lactamases among the identified ESBLs. Furthermore, most of the ESBL-producing E. coli isolates were multi-drug resistant. Residual antimicrobials such as sulfamethoxazole, trimethoprim, sulfadimidine, cephalexin, and sulfadiazine were also detected at a high level in both animal meats and environmental water collected from several cities, including Ho Chi Minh city and Can Tho city. These recent studies indicated that improper use of antimicrobials in animal-originated food production might contribute to the emergence and high prevalence of ESBL-producing E. coli in Vietnam. Although clonal ESBL-producing E. coli was not identified, CTX-M-55 gene-carrying plasmids with similar sizes (105-139 kb) have been commonly detected in the ESBL-producing E. coli strains isolated from various food animals and human beings. This finding strongly suggests that horizontal transfer of the CTX-M plasmid among various E. coli strains played a critical role in the emergence and high prevalence of ESBL-producing E. coli in Vietnam.

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Spread of Antibiotic and Antimicrobial Susceptibility of ESBL-Producing <i>Escherichia coli</i> Isolated from Wild and Cultured Fish in the Mekong Delta, Vietnam

Cited by 21 publications

References 18 publications

Prevalence and Antimicrobial Resistance of Escherichia coli, Salmonella and Vibrio Derived from Farm-Raised Red Hybrid Tilapia (Oreochromis spp.) and Asian Sea Bass (Lates calcarifer, Bloch 1970) on the West Coast of Peninsular Malaysia

Prevalence and Antimicrobial Resistance of Escherichia coli, Salmonella and Vibrio Derived from Farm-Raised Red Hybrid Tilapia (Oreochromis spp.) and Asian Sea Bass (Lates calcarifer, Bloch 1970) on the West Coast of Peninsular Malaysia

Dissemination of CTX-M-Producing Escherichia coli in Freshwater Fishes From a French Watershed (Burgundy)

Prevalence of extended-spectrum β-lactamase-producing Escherichia coli and residual antimicrobials in the environment in Vietnam

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