Sprayable nanomicelle hydrogels and inflammatory bowel disease patient cell chips for development of intestinal lesion-specific therapy

Yoon, Hyun Joong; Lee, Songhyun; Kim, Tae Young; Yu, Seung Eun; Kim, Hye Seon; Chung, Yong Eun; Chung, Su Yun; Park, Suji; Shin, Yong Cheol; Wang, Eun Kyung; Noh, Jihye; Kim, Hyun Jung; Ku, Cheol Ryong; Koh, Hong; Kim, Chang-Soo; Park, Joon‐Sang; Shin, Young Min; Sung, Hak Joon

doi:10.1016/j.bioactmat.2022.03.031

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…An mPEG-PCL di-block copolymer was synthesized by ring-opening polymerization of CL (Sigma-Aldrich) and poly (ethylene glycol) methyl ether (mPEG; M n = 750, Sigma-Aldrich) in the presence of tin (II) 2-ethylhexanoate [Sn (Oct) 2 , Sigma-Aldrich] as the catalyst [ 49 ]. Briefly, mPEG (10 mmol) and distilled CL (150 mmol) were prepared in a 3-necked round-bottom flask under a nitrogen atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Body-Shaping Membrane to Regenerate Breast Fat by Elastic Structural Holding

Kim

Park

et al. 2023

Research

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Tissue regeneration requires structural holding and movement support using tissue-type-specific aids such as bone casts, skin bandages, and joint protectors. Currently, an unmet need exists in aiding breast fat regeneration as the breast moves following continuous body motion by exposing the breast fat to dynamic stresses. Here, the concept of elastic structural holding is applied to develop a shape-fitting moldable membrane for breast fat regeneration (“adipoconductive”) after surgical defects are made. The membrane has the following key characteristics: (a) It contains a panel of honeycomb structures, thereby efficiently handling motion stress through the entire membrane; (b) a strut is added into each honeycomb in a direction perpendicular to gravity, thereby suppressing the deformation and stress concentration upon lying and standing; and (c) thermo-responsive moldable elastomers are used to support structural holding by suppressing large deviations of movement that occur sporadically. The elastomer became moldable upon a temperature shift above T m . The structure can then be fixed as the temperature decreases. As a result, the membrane promotes adipogenesis by activating mechanotransduction in a fat miniature model with pre-adipocyte spheroids under continuous shaking in vitro and in a subcutaneous implant placed on the motion-prone back areas of rodents in vivo.

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Section: Methodsmentioning

confidence: 99%

Body-Shaping Membrane to Regenerate Breast Fat by Elastic Structural Holding

Kim

Park

et al. 2023

Research

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“…Eight-week-old male C57BL/6 mice were randomly divided into 3 groups (9 animals for each group) as follows: the CON group, mice were fed normal drinking water; the DSS group, mice were fed 3% DSS in drinking water from Day 6 to Day 11) as the IBD model ( 15 ); and the DSS + IPA group, IPA(Sigma, CAS 830-96-6);at doses of 200 mg/kg body weight/day dissolving in 0.5% arboxymethyl cellulose sodium salt (CMC) was administered to mice by oral gavage and 3% DSS in the drinking water from Day 6 to Day 11. All mice were given free access to food and drinking water.…”

Section: Methodsmentioning

confidence: 99%

Pretreatment with IPA ameliorates colitis in mice: Colon transcriptome and fecal 16S amplicon profiling

Hou

et al. 2022

Front. Immunol.

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3-Indolepropionic acid (IPA) is a tryptophan metabolite that has anti-inflammatory properties. The present study try to investigate the phylactic effects of IPA on dextran sodium sulfate (DSS)-induced colitis mice. The results showed that IPA pretreatment ameliorated the DSS-induced decrease in growth performance, and intestinal damage and enhanced immunity in mice. RNA-seq analysis of mouse colon samples revealed that the differentially expressed genes (DEGs) were mainly enriched in immune-related pathways. 16S rRNA sequencing showed that IPA pretreatment ameliorated DSS-induced colonic microbiota dysbiosis. Moreover, the expression levels of gut immune genes were positively correlated with the relative abundance of several probiotics, such as Alloprevotella and Catenibacterium. In conclusion, IPA alleviates DSS-induced acute colitis in mice by regulating inflammatory cytokines, balancing the colonic microbiota and modulating the expression of genes related to inflammation, which would also provide a theoretical basis for IPA as a strategy to improve intestinal health.

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“…Shin et al developed an anoxic-oxic interface-on-a-chip by leveraging a modified human gut-on-a-chip. Furthermore, the results demonstrated a controlled oxygen gradient in the lumen-capillary transepithelial 86 Glass-based chip 87 The embedded electrodes for measuring the TEER 88 Using clinical IBD patient cells 89 Introduction of a collagen scaffold and using gravity flow device 83 interface by flowing anoxic and oxic culture media in various physiological environments. 85 Many studies focused on improving the throughput of gut-on-a-chip.…”

Section: Development Of Gut-on-a-chipmentioning

confidence: 99%

“…The data showed that peptidehydrogel treatment for 96 h induced significant structural recovery of IBD patient cells in gut-on-a-chip, supported by improved villi formation and ZO-1 expression. 89 It is also a new method to examine IBD based on the interaction between the microbiota and IBD. Significant variations in intestinal microbiota have been associated with IBD.…”

Section: Disease Models Using Human Gut-on-a-chipmentioning

confidence: 99%

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Gut-on-a-chip for disease models

et al. 2023

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The intestinal tract is a vital organ responsible for digestion and absorption in the human body and plays an essential role in pathogen invasion. Compared with other traditional models, gut-on-a-chip has many unique advantages, and thereby, it can be considered as a novel model for studying intestinal functions and diseases. Based on the chip design, we can replicate the in vivo microenvironment of the intestine and study the effects of individual variables on the experiment. In recent years, it has been used to study several diseases. To better mimic the intestinal microenvironment, the structure and function of gut-on-a-chip are constantly optimised and improved. Owing to the complexity of the disease mechanism, gut-on-a-chip can be used in conjunction with other organ chips. In this review, we summarise the human intestinal structure and function as well as the development and improvement of gut-on-a-chip. Finally, we present and discuss gut-on-a-chip applications in inflammatory bowel disease (IBD), viral infections and phenylketonuria. Further improvement of the simulation and high throughput of gut-on-a-chip and realisation of personalised treatments are the problems that should be solved for gut-on-a-chip as a disease model.

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Sprayable nanomicelle hydrogels and inflammatory bowel disease patient cell chips for development of intestinal lesion-specific therapy

Cited by 10 publications

References 32 publications

Body-Shaping Membrane to Regenerate Breast Fat by Elastic Structural Holding

Body-Shaping Membrane to Regenerate Breast Fat by Elastic Structural Holding

Pretreatment with IPA ameliorates colitis in mice: Colon transcriptome and fecal 16S amplicon profiling

Gut-on-a-chip for disease models

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