Pretreatment with IPA ameliorates colitis in mice: Colon transcriptome and fecal 16S amplicon profiling

Abstract

3-Indolepropionic acid (IPA) is a tryptophan metabolite that has anti-inflammatory properties. The present study try to investigate the phylactic effects of IPA on dextran sodium sulfate (DSS)-induced colitis mice. The results showed that IPA pretreatment ameliorated the DSS-induced decrease in growth performance, and intestinal damage and enhanced immunity in mice. RNA-seq analysis of mouse colon samples revealed that the differentially expressed genes (DEGs) were mainly enriched in immune-related pathways. 16S rRNA sequencing showed that IPA pretreatment ameliorated DSS-induced colonic microbiota dysbiosis. Moreover, the expression levels of gut immune genes were positively correlated with the relative abundance of several probiotics, such as Alloprevotella and Catenibacterium. In conclusion, IPA alleviates DSS-induced acute colitis in mice by regulating inflammatory cytokines, balancing the colonic microbiota and modulating the expression of genes related to inflammation, which would also provide a theoretical basis for IPA as a strategy to improve intestinal health.

Protective effect of Pediococcus pentosaceus Li05 on diarrhea-predominant irritable bowel syndrome in rats

“…[44][45][46] After Li05 intervention, the relative abundance of Mucispirillum significantly increased, alongside a significant decrease in the relative abundance of the inflammation-associated microbes Blautia and Erysipelatoclostridium, suggesting its protective effects against IBS-D. Additionally, the relative abundance of Alloprevotella has been positively correlated with the expression levels of intestinal immune-related genes and an increase in inflammatory bowel disease (IBD). 47,48 However, our data results showed a decreased relative abundance of Alloprevotella in IBS-D, and additional research is required to clarify the specific role in IBS-D.…”

Relevance of Carcinogen-Induced Preclinical Cancer Models

“…This results in inflammation, ulceration, and bleeding in the colon, similar to that in human ulcerative colitis, as observed in Figure 6 A. DSS mouse colitis can be induced by administering mice with DSS in their water for a certain period, usually between 5 and 7 days, depending on the dose and the strain of mice. The severity of colitis can be measured by various methods, such as measuring weight loss and colon shrinkage, examining the colon tissue for damage under a microscope, and testing the blood or colon tissue for levels of inflammatory substances that are produced by the immune system [ 46 ]. DSS mouse colitis is a useful model for studying the causes and treatments of IBD, as well as the interactions between the bacteria that live in the gut, the immune system that defends the body, and the brain that controls the behavior and mood of the mice.…”

The role of complex interactions between the intestinal flora and host in regulating intestinal homeostasis and inflammatory bowel disease

“…High levels of 5-HT promote the production of inflammatory factors and tight junctions and the breakage of tight junctions by activating NOX2, making it easier for E. coli to invade ( Banskota et al, 2017 ). In addition, IPA reversed the increased ratio of thick-walled bacteria/mimics in DSS mice, decreased the abundance of Aspergillus and increased the abundance of Lactococcus ( Fu et al, 2022 ).…”