Spray-on-skin cells in burns: A common practice with no agreed protocol

Allouni, Ammar; Papini, Remo; Lewis, Darren

doi:10.1016/j.burns.2013.03.017

Cited by 13 publications

(7 citation statements)

References 10 publications

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“…In clinical practice, the need for adequate cell-spraying protocols39 is even more pressing than expected. In particular, manual operation of spray devices is common practice, but associated with variations in nozzle-substrate distance, the air pressure, and the viscosity.…”

Section: Discussionmentioning

confidence: 99%

Optimizing cell viability in droplet-based cell deposition

Hendriks

Visser

Henke

et al. 2015

Sci Rep

101

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Biofabrication commonly involves the use of liquid droplets to transport cells to the printed structure. However, the viability of the cells after impact is poorly controlled and understood, hampering applications including cell spraying, inkjet bioprinting, and laser-assisted cell transfer. Here, we present an analytical model describing the cell viability after impact as a function of the cell-surrounding droplet characteristics. The model connects (1) the cell survival as a function of cell membrane elongation, (2) the membrane elongation as a function of the cell-containing droplet size and velocity, and (3) the substrate properties. The model is validated by cell viability measurements in cell spraying, which is a method for biofabrication and used for the treatment of burn wounds. The results allow for rational optimization of any droplet-based cell deposition technology, and we include practical suggestions to improve the cell viability in cell spraying.

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Section: Discussionmentioning

confidence: 99%

Optimizing cell viability in droplet-based cell deposition

Hendriks

Visser

Henke

et al. 2015

Sci Rep

101

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“…Another promising delivery technology is cell spraying that allows clinicians to treat large deep burns [61, 207, 208]. In most studies, scientists used autologous epidermis-derived cells.…”

Section: Delivery Approachesmentioning

confidence: 99%

Skin tissue regeneration for burn injury

et al. 2019

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The skin is the largest organ of the body, which meets the environment most directly. Thus, the skin is vulnerable to various damages, particularly burn injury. Skin wound healing is a serious interaction between cell types, cytokines, mediators, the neurovascular system, and matrix remodeling. Tissue regeneration technology remarkably enhances skin repair via re-epidermalization, epidermal-stromal cell interactions, angiogenesis, and inhabitation of hypertrophic scars and keloids. The success rates of skin healing for burn injuries have significantly increased with the use of various skin substitutes. In this review, we discuss skin replacement with cells, growth factors, scaffolds, or cell-seeded scaffolds for skin tissue reconstruction and also compare the high efficacy and cost-effectiveness of each therapy. We describe the essentials, achievements, and challenges of cell-based therapy in reducing scar formation and improving burn injury treatment.

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“…The use of epidermal cell suspensions (“spray-on skin”) in burn patients has been called a “common practice with no agreed protocol” with regard to indications for its use, techniques used, dressings to be used, timing of first wound review, and outcome measures [21]. In principle, two techniques are used.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

Andreone¹,

Hollander

2019

Stem Cells International

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The coverage of massive burns still represents a big challenge, even if several strategies are to date available to deal with this situation. In this study, we describe the use of a combination of platelet-rich fibrin and micrograft spray-on skin in order to increase the yield of grafted cells in patients. We treated a total of five patients, of which two were affected by massive burns and three with chronic burn wounds. Briefly, autologous micrografts were obtained by Rigenera technology using a class I medical device called Rigeneracons. The micrografts were then combined with PRF and sprayed on the wound bed by a Spraypen. Before applying PRF/micrograft spray-on skin, the wound bed was covered with an Integra® dermal template, and the wounds were dressed with a layer of antimicrobial dressing applied directly over the silicone layer. When the silicone layer of the dermal template started showing signs of separation, the wound was considered ready for grafting. In all cases, we observed a fast and complete skin graft on average after 7-10 days by PRF/micrograft spray-on skin treatment. In particular, two patients with massive burns reported rapid reepithelialization, and three patients with chronic burn wounds, two of whom had failed skin grafts before the procedure, had complete wound healing within a week. In conclusion, the results showed in this study suggest that the use of PRF/micrograft spray-on skin represents a promising approach in the management of burns or chronic burn wounds.

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Spray-on-skin cells in burns: A common practice with no agreed protocol

Cited by 13 publications

References 10 publications

Optimizing cell viability in droplet-based cell deposition

Optimizing cell viability in droplet-based cell deposition

Skin tissue regeneration for burn injury

A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

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