A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

Abstract

The coverage of massive burns still represents a big challenge, even if several strategies are to date available to deal with this situation. In this study, we describe the use of a combination of platelet-rich fibrin and micrograft spray-on skin in order to increase the yield of grafted cells in patients. We treated a total of five patients, of which two were affected by massive burns and three with chronic burn wounds. Briefly, autologous micrografts were obtained by Rigenera technology using a class I medical device called Rigeneracons. The micrografts were then combined with PRF and sprayed on the wound bed by a Spraypen. Before applying PRF/micrograft spray-on skin, the wound bed was covered with an Integra® dermal template, and the wounds were dressed with a layer of antimicrobial dressing applied directly over the silicone layer. When the silicone layer of the dermal template started showing signs of separation, the wound was considered ready for grafting. In all cases, we observed a fast and complete skin graft on average after 7-10 days by PRF/micrograft spray-on skin treatment. In particular, two patients with massive burns reported rapid reepithelialization, and three patients with chronic burn wounds, two of whom had failed skin grafts before the procedure, had complete wound healing within a week. In conclusion, the results showed in this study suggest that the use of PRF/micrograft spray-on skin represents a promising approach in the management of burns or chronic burn wounds.

Cartilage Micrografts as a Novel Non-Invasive and Non-Arthroscopic Autograft Procedure for Knee Chondropathy: Three-Year Follow-Up Study

“…To date, the Rigenera ® micrografting technology has demonstrated its efficacy in different clinical settings. It is widely used with dermal tissue samples for the treatment of complex wounds such as dehiscences, chronic ulcers, and burns [ 11 , 12 , 13 , 14 , 15 , 16 ], for dermatological applications such as hypertrophic scars [ 17 ], and for the management of androgenetic alopecia [ 18 , 19 ], with cartilage samples for the management of chondropathy [ 20 , 21 ], with bone samples for the treatment of the osteonecrosis of the femoral head [ 22 ].…”

Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts

“…Furthermore, PRF has been utilized as an adjuvant for peri-implantitis therapy [3][4][5]. In general medicine, PRF has also been utilized to promote the healing and wound closure of chronic ulcers [6] and resurfacing of full-thickness burns [7]. The beneficial effects can be explained by the PRF intrinsic growth factors triggering cell responses of PRF including its ability to promote cell proliferation, migration, and differentiation [8,9].…”

Evaluation of Bone Regeneration in Rat Calvaria Using Bone Autologous Micrografts and Xenografts: Histological and Histomorphometric Analysis

“…To date, the Rigenera® micrografting technology has demonstrated its efficacy in different clinical setting. It is widely used with dermal tissue sample for the treatment of complex wounds, such as dehiscences, chronic ulcers, and burns [ 17 , 18 , 19 , 20 , 21 , 22 ]; for dermatological applications, such as hypertrophic scars [ 23 ]; and for the management of androgenetic alopecia [ 24 , 25 ]; with cartilage samples for the management of chondropathy [ 26 , 27 ]; and with bone samples for the treatment of the osteonecrosis of the femoral head [ 28 ].…”