SPR Studies of the Nonspecific Adsorption Kinetics of Human IgG and BSA on Gold Surfaces Modified by Self-Assembled Monolayers (SAMs)

Silin, V. P.; Weetall, Howard H.; Vanderah, David J.

doi:10.1006/jcis.1996.4586

Cited by 331 publications

(310 citation statements)

References 6 publications

(10 reference statements)

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“…This work revealed that the highest protein adsorption occurred on surfaces containing the highest amount of hydroxyl groups on the surface (from the 2.5% HEMA surfaces). This was initially surprising, as hydroxyl groups have been noted to be hydrophilic in nature and are generally correlated with low protein adsorption on such surfaces as hydroxyl terminated self assembled monolayers (SAMs) [22,[40][41][42]. However, others have found that proteins such as fibrinogen and albumin can bind to hydroxylated surfaces.…”

Section: Discussionmentioning

confidence: 99%

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

et al. 2008

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Nafion™ is the membrane material preferred for in situ glucose sensors. Unfortunately, surface properties of Nafion promote random protein adsorption and eventual foreign body encapsulation thus leading to loss if glucose signal over time. Here we detail surface modifications made by RF plasma deposition to Nafion with the intent to prevent random protein adsorption while providing enough functional sites (hydroxyl groups) to bind a biologically active peptide known to induce cellular adhesion (YRGDS). Nafion surfaces were modified by RF plasma polymerizing five different combinations of (1) tetraethylene glycol dimethyl ether (tetraglyme) and (2) 2-hydroxyethyl methacrylate (HEMA): pure tetraglyme, 2.5% HEMA/97.5% tetraglyme; 5% HEMA/95% tetraglyme, 10% HEMA/90% tetraglyme; and pure HEMA. Resultant surfaces were characterized by XPS (low and high resolution), dynamic contact angle, and atomic force microscopy. Protein adsorption and retention was determined and correlated to surface layer composition. The ability to bind a cell adhesion peptide was also determined and correlated well with surface layer composition.

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Section: Discussionmentioning

confidence: 99%

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

et al. 2008

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“…They postulated that the protein adsorption to this type of surface was a multistep process, probably initiated by interaction of hydrophobic residues, that have temporarily become exposed at the surface of the protein, with the hydrophobic surface. This initial interaction is then followed by multipoint interactions due to various degrees of protein denaturation, making desorption from the surface extremely difficult (Silin, V et al, 1997). The experiments of our group indicated that the biological activity of the antibodies was not hampered (Bijnens et al, 2009).…”

Section: Antibodiesmentioning

confidence: 95%

“…Our group constructed an impedimetric immunosensor directed against C-Reactive Protein (CRP), an acute phase protein serving as a marker for cardiovascular disease, based on the physical adsorption of anti-CRP to H-terminated nanocrystalline diamond (NCD), since Silin et al (1997) demonstrated the suitability of hydrophobic surfaces for antibody adsorption. They postulated that the protein adsorption to this type of surface was a multistep process, probably initiated by interaction of hydrophobic residues, that have temporarily become exposed at the surface of the protein, with the hydrophobic surface.…”

Section: Antibodiesmentioning

confidence: 99%

Evolution towards the implementation of point-of-care biosensors

Vermeeren¹,

Michiels²

2011

Biosensors for Health, Environment and Biosecurity

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“…First, hydroxyl-terminated SAMs have a lower level of non-specific analyte binding than carboxyl-terminated SAMs (Silin et al, 1997). Second, the steric hindrance (Patel et al, 1998;Lee et al, 2005) and electrostatic repulsion of the acid thiols in mixed SAMs are lower than in SAMs consisting of acid thiols only.…”

Section: Detection Of Rca 120 On Sam-1 -2 -3 and -4mentioning

confidence: 99%

The application of neoglycopeptides in the development of sensitive surface plasmon resonance-based biosensors

Maljaars

Souza

Halkes

et al. 2008

Biosensors and Bioelectronics

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a b s t r a c tThe development of a biosensor based on surface plasmon resonance is described for the detection of carbohydrate-binding proteins in solution on a Biacore 2000 instrument, using immobilized glycopeptides as ligands. Their selection was based on previous screenings of solid-phase glycopeptide libraries with Ricinus communis agglutinin (RCA 120 ) and human adhesion/growth-regulatory galectin-1 (h-Gal-1). Glycopeptides were immobilized on Au sensor chips functionalized with mixed self-assembled monolayers of different ratios of 11-mercapto-1-undecanol and 11-mercaptoundecanoic acid, and of 3-mercapto-1-propanol and 11-mercaptoundecanoic acid. The biosensors were optimized for the detection of RCA 120 , and a detection limit of 0.13 nM was obtained. Subsequent experiments with h-Gal-1 indicated a detection limit of at least 0.9 nM for this lectin. Additionally, the effect of interfering proteins on the sensitivity of the optimized biosensor was investigated.

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SPR Studies of the Nonspecific Adsorption Kinetics of Human IgG and BSA on Gold Surfaces Modified by Self-Assembled Monolayers (SAMs)

Cited by 331 publications

References 6 publications

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

Surface modification of a perfluorinated ionomer using a glow discharge deposition method to control protein adsorption

Evolution towards the implementation of point-of-care biosensors

The application of neoglycopeptides in the development of sensitive surface plasmon resonance-based biosensors

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