SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum

Abstract

A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006-70 mg L and a limit of detection of 0.009 mg L were achieved. These results are among the best compared to other SPR-based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically-relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real-time, label-free, portable and low cost diagnosis of CRP for future lab-on-a-chip applications. 3D sketch (left) of the thermo-stabilized home-made flow cell developed to house the SPR-based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C-reactive protein in serum.

Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

“…Over the past years, a FO-SPR biosensor was developed as a cost-effective and easy-to-use alternative for the more expensive and complex SPR systems [ 17 ], such as the well-known commercially available Biacore instrument (GE Healthcare, Uppsala, Sweden). The FO-SPR biosensor makes use of multimode optical fibers that facilitate SPR generation and enable implementation of bioassays [ 18 , 19 , 20 ]. As has already been demonstrated by our group, the FO-SPR biosensor developed in-house has the unique capacity to quantify a variety of different molecules, ranging from proteins and nucleic acids to small molecules not only in the buffer but also in the complex sample matrices, such as whole blood, serum, plasma or milk [ 14 , 21 , 22 , 23 , 24 ].…”

A sensitive tapered-fiber optic biosensor for the label-free detection of organophosphate pesticides

“…In fact, the important measurand is represented by the surface RI changes induced by the interactions between the immobilized captures (AChE) and the target analytes (OP pesticide) along the functionalized surface, and not by the bulk RI changes related to the refractive index of the solution pumped inside the flow cell [39]. Therefore, the signal value after this washing step is considered as the real shift related to each MPT concentration.…”

“…the measurement achieved with PBS right before applying pesticide [39,41]. From the calibration curve of Fig.…”

Dual-polarized highly sensitive plasmonic sensor in the visible to near-IR spectrum

“…During the performance analysis we choose RI from 1.33 to 1.43. This is because the RI of biochemical interactions goes from 1.33 to 1.35 however the range 1.33 to 1.43 is considered based on practical bio-chemical and chemical sensing [60,61]. It is observed that the resonance peak shift increases with the increase of analyte RI resulting in high sensitivity for larger RI.…”