Spotting of External Calibration Standards on Blank Dried Blood Spots as A Resource-Sparing Protocol

Abstract

Blank DBS samples can be opportunistically prepared from overweight or satellite animals, be stored, and subsequently spiked with standards to prepare calibration standards.

Quantitative microsampling for bioanalytical applications related to the SARS-CoV-2 pandemic: Usefulness, benefits and pitfalls

“… LC-HRMS [ 114 ] VAMS Blood Hydroxychloroquine 10 LC-MS/MS [ 115 ] DBS Blood Hydroxychloroquine n.s. LC-MS/MS [ 116 ] DBS Blood Li + 50 GF-AAS [ 118 ] DPS Plasma Li + 20 GF-AAS [ 118 ] DBS Blood Cyclosporin A n.s. LC-MS/MS [ 120 ] Abbreviations : AAS, Atomic Absorption Spectrometry; BD, Blood Drop (not dried); BFS, BioFluid Sampler; BMS, Bronchoscopic MicroSample; DBS, Dried Blood Spots; DMKS, Dried MilK Spots; DPS, Dried Plasma Spots; DOFS, Dried Oral Fluid Spots; DUS, Dried Urine Spots; EBC, Exhaled Breath Condensate; ELF, Epithelial Lining Fluid; FL, FLuorescence detection; FPSE, Fabric-Phase Sorptive Extraction; GF, Graphite Furnace; HRMS, High-Resolution Mass Spectrometry; LC, Liquid Chromatography or high-performance Liquid Chromatography; MS/MS, tandem Mass Spectrometry; NB, Northern Blotting; n.s., not specified (for BD and DBS it usually ranges from 50 to 200 μL); NSAID, Non-Steroidal Anti-Inflammatory Drug; PCR, Polymerase Chain Reaction; PS, Paper Spray; SB, Southern Blotting; SPE, Solid-Phase Extraction; SPME, Solid-Phase MicroExtraction; UHPLC, Ultra-High-Performance Liquid Chromatography; VAMS, Volumetric Absorptive MicroSampling.…”

“…Regarding hydroxychloroquine, VAMS has been used for its determination in rheumatoid arthritis patients [ 115 ], while DBS has been used for a pharmacokinetic study in rats [ 116 ].…”