Spotted-Leaf Mutants of Rice (Oryza sativa)

Huang, Qi-na; Yang, Yang; Shí, Yùzhēn; Chen, Jie; Wu, Jianli

doi:10.1016/s1672-6308(09)60024-x

Cited by 26 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From 120,000 M 2 lines of 9311, Nipponbare and Wuyunjing7 rice mutagenized with low-and medium-energy heavy ions, we identified five mutants showing lesion-mimic phenotypes on leaves, termed spl29-spl33 [10]. The spl29 and spl30 were of the 9311 variety (low-energy heavy ions), spl31 and spl32 were Nipponbare (medium-energy heavy ions) and spl33 was Wuyunjing7 (medium-energy heavy ions).…”

Section: Isolation and Phenotypic Analysis Of Spl Mutantsmentioning

confidence: 99%

“…Plant spotted-leaf (spl) mutants are a class of mutants that spontaneously display lesions in the absence of any obvious injury, stress or pathogen infection [9]. There are many ways that spl mutants are produced, such as natural mutation, fast neutrons, c-ray, T-DNA insertion and others [10]. Rice spotted-leaf mutants induced by heavy ions, however, have not been previously described.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mutagenic effects of heavy ion irradiation on rice seeds

Xue¹,

Liu²,

Zhang³

et al. 2012

Nuclear Instruments and Methods in Physics Research Section B:

View full text Add to dashboard Cite

Section: Isolation and Phenotypic Analysis Of Spl Mutantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutagenic effects of heavy ion irradiation on rice seeds

Xue¹,

Liu²,

Zhang³

et al. 2012

Nuclear Instruments and Methods in Physics Research Section B:

View full text Add to dashboard Cite

“…To date, at least 55 LMMs have been reported in rice (http://www.gramene.org/) among which, most are controlled by recessive genes and only a fraction by dominant or semidominant genes (Huang et al, 2010). Many of these LMMs show substantially increased resistance to certain pathogen-caused diseases.…”

Section: Fine Mapping Of a New Lesion Mimic And Early Senescence 2 (Lmentioning

confidence: 99%

Fine Mapping of a New Lesion Mimic and Early Senescence 2 (lmes2) Mutant in Rice

Xing

Xiao

et al. 2016

Crop Science

View full text Add to dashboard Cite

A novel lesion mimic and early senescence 2 (lmes2) mutant was identified from Jinhui10, an indica restorer line in rice (Oryza sativa L.), which was treated with ethyl methane sulfonate (EMS). Lmes2 began to develop disease‐like lesions on leaves at the seedling stage and aged leaves that had the maximum distribution of such lesions began to senesce. Compared with the wild‐type, a low content of chlorophylls was observed, while the proline content increased in proportion to the development of lesion mimics. In addition, the photosynthetic rate and superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities fell very significantly, which indicates that lmes2 develops early senescence. Excessive hydrogen peroxide (H2O2) deposition and trypan blue staining imply that lmes2 goes through programmed cell death. A population of F2 was used to fine map LMES2 to a 66‐kb region on chromosome 10, which harbors 10 open reading frames. Quantitative real‐time polymerase chain reaction results show the activation of two disease resistance indicator genes, PAL and PBZ1, in the process of lesion formation; this finding indicates that resistance activity may have activated. Our results provide a foundation for finding and functional analysis of the novel lesion mimic mutant (LMM) gene, LMES2.

show abstract

“…Rice LMMs have been isolated from natural variants or by chemical and physical mutagenesis, and more than 100 rice LMMs have been found to date (Liu et al 2003). Genetic analysis has indicated that the majority of rice LMMs are controlled by single recessive or dominant genes (Wu et al 2008;Huang et al 2010;2011). Seven rice lesion mimic (or spotted leaf) genes have been cloned: Spl7, Spl11, OsLSD1, OsAT1, OsPti1a, Spl28 and SL (Yamanouchi et al 2002;Zeng et al 2004;Wang et al 2005;Mori et al 2007;Takahashi et al 2007;Qiao et al 2010;Fujiwara et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Rice LMMs display enhanced or decreased disease resistance compared with their wild-type parents (Takahashi et al 1999;Campbell & Ronald 2005;Huang et al 2010). However, the disease resistance of some mutants is not altered, suggesting that the lesion mutations either reside in the developmental cell death pathway or represent certain physiological disorders that are irrelevant to defence responses (Zeng et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Fine mapping and candidate gene analysis of LM3, a novel lesion mimic gene in rice

Zeng

et al. 2012

Biologia

View full text Add to dashboard Cite

A rice lesion mimic mutant, lm3, was obtained by the mutagenesis of an indica cultivar, 93-11, using γ-ray radiation. Brownish lesions appeared on the leaves of lm3 at the young seedling stage and persisted until the ripening stage. The lm3 mutant was characterised by a shorter plant height and delayed heading compared with the wild-type 93-11. A genetic analysis indicated that the lesion mimic phenotype was controlled by a single recessive gene. Using simple sequence repeat (SSR) markers, the target gene LM3 was first located between marker RM5748 and RM14906 on chromosome 3. We then developed Insertion-Deletion (InDel) markers to fine-map LM3, and the locus was localised to a 29 kb region defined by two InDel markers, In12571 and In12600. Five ORFs were predicted in the candidate region, and DNA sequencing detected a single-nucleotide polymorphism (SNP) in the coding region of LOC Os03g21900. The SNP in the fourth exon (C in 93-11; T in lm3) of LOC Os03g21900 results in the substitution of a proline (P) with a serine (S) at the 140 th amino acid of the deduced uroporphyrinogen decarboxylase protein. We did not detect polymorphisms in the other predicted ORF regions between lm3 and 93-11. These results suggest that LOC Os03g21900 is the most likely candidate gene for LM3.

show abstract

Spotted-Leaf Mutants of Rice (Oryza sativa)

Cited by 26 publications

References 51 publications

Mutagenic effects of heavy ion irradiation on rice seeds

Mutagenic effects of heavy ion irradiation on rice seeds

Fine Mapping of a New Lesion Mimic and Early Senescence 2 (lmes2) Mutant in Rice

Fine mapping and candidate gene analysis of LM3, a novel lesion mimic gene in rice

Contact Info

Product

Resources

About