Mutagenic effects of heavy ion irradiation on rice seeds

Xue, Xu; Liu, Binmei; Zhang, Lili; Wu, Yuejin

doi:10.1016/j.nimb.2012.08.028

Cited by 7 publications

(4 citation statements)

References 30 publications

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“…These varieties can be considered resistant to plasma irradiation. The results were in accordance with Sasikala and Kalaiyarasi [11], who studied sensitivity of rice varieties to gamma irradiation in 6 rice varieties; the survival percentages were decreased when the dosages of radiation increased, but the responses of all 6 rice varieties had different survival percentages. The different results may be due to different rice species having different radiation responses and different radiation types.…”

Section: Effects Of Plasma Focus On Seed Germinationsupporting

confidence: 89%

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Effects of Plasma Focus on Seed Germination and Seedling Growth of 14 Thai Rice Varieties

Puripunyavanich¹,

Tamman²,

Orpong³

et al. 2021

Walailak J Sci & Tech

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Thailand Plasma Focus 2 (TPF-2) is operated at 2.16 kJ of storage energy with argon as a filled gas and can emit sharp x-ray pulses to plant cells in nanosecond (10-9 s) duration. The effects of plasma focus x-ray on seed germination and seedling growth of 14 Thai rice varieties treated under 10 conditions; 0 (control), 2, 4, 6, 8, 10, 12, 14, 16, and 18 plasma focus shots, were investigated. The results show that the germination rates and growth reduction rates were significantly different among the rice varieties. The germination rate of 10 varieties (Jek Chuey, KDML105, LPT123, PTT1, RD41, RD47, RD49, RD67, RD79, and SPR2) decreased when treated with a high number of plasma focus shots. LD50 and GR50 values were calculated, except for KTH17, RD1, RD7, and RD31 whose germination only slightly decreased, or remained constant, even after they were treated by 18 plasma focus shots. HIGHLIGHTS Effects of plasma focus irradiation on germination and growth were assessed on 14 Thai rice varieties Shoot and root lengths of rice seedlings were measured 10 days after of plasma treatment Plasma focus was operated at 12 kV and the number of plasma focus shots was varied from 0 (control) to 18 shots Sensitivity to plasma irradiation was found to differ among rice seeds tested GRAPHICAL ABSTRACT

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Section: Effects Of Plasma Focus On Seed Germinationsupporting

confidence: 89%

“…Chronic gamma irradiation produced inhibitory effects on rice plant height. Sasikala and Kalaiyarasi [11] showed that seedling height decreased with increase of gamma irradiation dose. The 2 nd group, which showed high resistance to plasma shots, consisted of KTH17, RD1, RD7, and RD31.…”

Section: Effects Of Plasma Focus On Growth Ratementioning

confidence: 99%

Effects of Plasma Focus on Seed Germination and Seedling Growth of 14 Thai Rice Varieties

Puripunyavanich¹,

Tamman²,

Orpong³

et al. 2021

Walailak J Sci & Tech

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“…An mtl1 mutant was obtained from the japonica cultivar WYJ7 via mutagenesis treatment with a heavy ion beam in 2015. The methods used for mutagenesis have been described by Xu et al (2012) [28]. The parameters used in the present study were as follows: an ion energy of 25 keV; N + ion fluence of 2.5 × 10 16 ions/cm 2 ; an irradiation time of 10 min [28].…”

Section: Materials Planting and Investigation Of Agronomic Traitsmentioning

confidence: 99%

“…The methods used for mutagenesis have been described by Xu et al (2012) [28]. The parameters used in the present study were as follows: an ion energy of 25 keV; N + ion fluence of 2.5 × 10 16 ions/cm 2 ; an irradiation time of 10 min [28]. In May 2021, the seeds of WYJ7 and mtl1 were germinated under dark conditions in an oven at 37 • C. After sprouting, the seeds were spread on the seedling bed at the Anhui Science and Technology University, Fengyang Campus, Anhui Province (32 • 52 30 N, 117 • 33 15 E).…”

Section: Materials Planting and Investigation Of Agronomic Traitsmentioning

confidence: 99%

Deletion of the OsLA1 Gene Leads to Multi-Tillering and Lazy Phenotypes in Rice

Sun,

Mei,

Feng

et al. 2023

Agriculture

Self Cite

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Plant architecture, one of the key factors that determine grain yield in rice, is mainly affected by components such as plant height, tiller number, and panicle morphology. For this paper, we obtained a multi-tillering and lazy mutant from a japonica rice cultivar, Wuyunjing 7 (WYJ7), via treatment with a heavy ion beam. Compared to WYJ7, the mutant showed a significant increase in tiller angle, tiller number, number of primary and secondary branches, and number of grains; however, the plant height and grain thickness of the mutant was significantly decreased. Phenotypic analysis of the F1 hybrids revealed that the multi-tillering and lazy mutant phenotypes were regulated by a recessive gene. The segregation ratio of 1׃3 of the mutant phenotype and the wild-type plant in the F2 population indicated that the former was controlled by a single gene named Multi-Tillering and Lazy 1 (MTL1). Bulked segregant analysis was performed using the individual plants with extremely typical tiller angles in the F2 population. The MTL1 gene was initially mapped within a region of 5.58–17.64 Mb on chromosome 11. By using the F2 segregated population for fine mapping, the MTL1 gene was ultimately fine mapped within the range of 66.67 kb on chromosome 11. The analysis of genes in this region revealed the presence of the previously identified LAZY1 (LA1) gene. Genomic PCR amplification and semi-quantitative RT-PCR assays showed that the LA1 gene could not be amplified and was not expressed, thus indicating that the MTL1 gene might be identical to the LA1 gene. This study suggests that the multi-tillering and lazy mutant phenotypes might be caused by the deletion of LA1 function. This finding can guide further investigations on the functional mechanisms of the LA1 gene, thus enriching the theoretical knowledge of plant architecture in relation to rice.

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