Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Silva, Fábio Sérgio Barbosa da; Yano‐Melo, Adriana Mayumi; Brandão, Joana Angélica C.; Maia, Leonor Costa

doi:10.1590/s1517-83822005000400004

Cited by 15 publications

(15 citation statements)

References 27 publications

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“…Hyphal length (5–11 m cm −3 substrate) was similar to values observed by Neumann et al (2009) in a comparable substrate (3.5–8.5 m cm −3 substrate) and was also within the range obtained by Drew et al (2006) in a sand substrate. Spore density was up to five times higher than values reported for pot cultures using a 1:1 sand/vermiculite substrate (up to 200 spores cm −3 substrate) (Silva et al 2005) and ten times the values reported from field samples (up to 100 spores cm −3 substrate) (Oehl et al 2005), but within the range reported by Neumann et al (2009). In the present study, ERM growth in the FC was clearly decreased by the supply of NH 4 + to the pot substrate.…”

Section: Discussionsupporting

confidence: 67%

Influence of different mineral nitrogen sources (NO−3-N vs. NH+4-N) on arbuscular mycorrhiza development and N transfer in a Glomus intraradices–cowpea symbiosis

2012

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Labeled nitrogen (15 N) was applied to a soil-based substrate in order to study the uptake of N by Glomus intraradices extraradical mycelium (ERM) from different mineral N (NO3− vs. NH4+) sources and the subsequent transfer to cowpea plants. Fungal compartments (FCs) were placed within the plant growth substrate to simulate soil patches containing root-inaccessible, but mycorrhiza-accessible, N. The fungus was able to take up both N-forms, NO3− and NH4+. However, the amount of N transferred from the FC to the plant was higher when NO3− was applied to the FC. In contrast, analysis of ERM harvested from the FC showed a higher 15 N enrichment when the FC was supplied with 15NH4+ compared with 15NO3−. The 15 N shoot/root ratio of plants supplied with 15NO3− was much higher than that of plants supplied with 15NH4+, indicative of a faster transfer of 15NO3− from the root to the shoot and a higher accumulation of 15NH4+ in the root and/or intraradical mycelium. It is concluded that hyphae of the arbuscular mycorrhizal fungus may absorb NH4+ preferentially over NO3− but that export of N from the hyphae to the root and shoot may be greater following NO3− uptake. The need for NH4+ to be assimilated into organically bound N prior to transport into the plant is discussed.

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Section: Discussionsupporting

confidence: 67%

Influence of different mineral nitrogen sources (NO−3-N vs. NH+4-N) on arbuscular mycorrhiza development and N transfer in a Glomus intraradices–cowpea symbiosis

2012

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“…etunicatum was shown to produce a high number of spores/g of soil, ranging from 18.6 to 152.8 , in symbiosis with Panicum miliaceum, whereas lower values (from 0.22 to 1.54 spores/g of soil), comparable to spore densities reported by this work, were observed in symbiosis with Sorghum vulgare (Silva et al, 2005).…”

Section: Greenhouse Experimentssupporting

confidence: 76%

Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation

Pellegrino¹,

Ramasamy²,

Sbrana³

et al. 2010

Ital J Agronomy

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Arbuscular mycorrhizal (AM) fungi play a key role in host plant growth and health, nutrient and water uptake, plant community diversity and dynamics. AM fungi differ in their symbiotic performance, which is the result of the interaction of two fungal characters, infectivity and efficiency. Infectivity is the ability of a fungal isolate to establish rapidly an extensive mycorrhizal symbiosis and is correlated with pre-symbiotic steps of fungal life cycle, such as spore germination and hyphal growth. Here, different AM fungal isolates were tested with the aim of selecting infective endophytes for field inoculation. Greenhouse and microcosm experiments were performed in order to assess the ability of 12 AM fungal isolates to produce spores, colonize host roots and to perform initial steps of symbiosis establishment, such as spore germination and hyphal growth. AM fungal spore production and root colonization were significantly different among AM fungal isolates. Spore and sporocarp densities ranged from 0.8 to 7.4 and from 0.6 to 2.0 per gram of soil, respectively, whereas root colonization ranged from 2.9 to 72.2%. Percentage of spore or sporocarp germination ranged from 5.8 to 53.3% and hyphal length from 4.7 to 79.8 mm. The ordination analysis (Redundancy Analysis, RDA) showed that AM fungal isolates (used as explanatory variables) explained about 60% of the whole variance and their effect on fungal infectivity variables was significant (P = 0.002). The biplot clearly showed that variables which might be used to detect infective AM fungal isolates were hyphal length and root colonization. Such analysis may allow the detection of the best parameters to select efficient AM fungal isolates to be used in agriculture.

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“…[41-43, 8, 44, 45]. Addition of organic residues to the substrate is known to increase AM fungal sporulation hence leading to increased inoculum production [46][47][48][49]. Douds et al [8] successfully produced AM fungal inoculum in compost mixed with vermiculite, perlite, or horticultural potting media and observed that the propagule numbers were maximum in vermiculite based media.…”

Section: Effect Of Different Carrier Treatments On Re-inoculation/colmentioning

confidence: 99%

Development of Carrier Based in Vitro Produced Arbuscular Mycorrhizal (AM) Fungal Inocula for Organic Agriculture

Rodrigues¹,

Rodrigues²

2017

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Abstract. Studies on the advantageous effects of arbuscular mycorrhizal (AM) fungi are providing new possible ways to exploit them as biofertilizers in sustainable agriculture. Many studies have described the potential of root organ culture (ROC) system for production of AM fungal inocula. However there is a need for development of a suitable carrier formulation to support in vitro produced AM fungal inocula when mixed with substrate, so as to enable the delivery of inocula in the rhizosphere. The aim of this study was to assess the performance of the organic carrier formulation consisting of vermiculite as the main component along with cattle manure, wood powder and wood ash in different proportions; and its ability to retain inoculum potential of the in vitro produced AM fungal propagules of Rhizoglomus intraradices and Funneliformis mosseae. Treatment 5 comprising of carrier formulation (vermiculite: cow dung powder: wood powder: wood ash) in the ratio of 20:8:2:1 was observed to be as the best carrier treatment for both the in vitro produced AM species. The in vitro produced propagules of both AM species were viable and effectively colonized the roots of Eleusine coracana Gaertn. The method established shows the efficiency of the carrier formulation in sustaining the inoculum potential of in vitro produced AM propagules for mass multiplication and possibility in application.

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Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Cited by 15 publications

References 27 publications

Influence of different mineral nitrogen sources (NO−3-N vs. NH+4-N) on arbuscular mycorrhiza development and N transfer in a Glomus intraradices–cowpea symbiosis

Influence of different mineral nitrogen sources (NO−3-N vs. NH+4-N) on arbuscular mycorrhiza development and N transfer in a Glomus intraradices–cowpea symbiosis

Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation

Development of Carrier Based in Vitro Produced Arbuscular Mycorrhizal (AM) Fungal Inocula for Organic Agriculture

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