Development of Carrier Based in Vitro Produced Arbuscular Mycorrhizal (AM) Fungal Inocula for Organic Agriculture

Abstract

Abstract. Studies on the advantageous effects of arbuscular mycorrhizal (AM) fungi are providing new possible ways to exploit them as biofertilizers in sustainable agriculture. Many studies have described the potential of root organ culture (ROC) system for production of AM fungal inocula. However there is a need for development of a suitable carrier formulation to support in vitro produced AM fungal inocula when mixed with substrate, so as to enable the delivery of inocula in the rhizosphere. The aim of this study was to assess the performance of the organic carrier formulation consisting of vermiculite as the main component along with cattle manure, wood powder and wood ash in different proportions; and its ability to retain inoculum potential of the in vitro produced AM fungal propagules of Rhizoglomus intraradices and Funneliformis mosseae. Treatment 5 comprising of carrier formulation (vermiculite: cow dung powder: wood powder: wood ash) in the ratio of 20:8:2:1 was observed to be as the best carrier treatment for both the in vitro produced AM species. The in vitro produced propagules of both AM species were viable and effectively colonized the roots of Eleusine coracana Gaertn. The method established shows the efficiency of the carrier formulation in sustaining the inoculum potential of in vitro produced AM propagules for mass multiplication and possibility in application.

Evaluation of municipal sewage sludge for Arbuscular mycorrhizal fungi inoculum production

“…For AMF proliferation, a well-aerated substrate deficient in nutrient is considered to be good (Gaur and Adholeya, 2000). The addition of organic residues as a substrate along with soil is known to promote AMF sporulation hence leading to increased inoculum production (Rodrigues and Rodrigues, 2017). Likewise, the addition of SS helped to maintain appropriate soil moisture content, aeration, and space which in turn provide feasible environment for the germination and sporulation of AMF.…”

“…In general, large scale propagation of AMF, multiplication under pot conditions is regarded as the most appropriate system (Feldmann and Grotkass, 2002). Furthermore, this soil can be amended with various other substrate materials to speed up the propagation process (Douds and Schenck, 1990;Gómez-Bellot et al, 2020) like sugarcane bagasse (Tanwar et al, 2013), cattle manure, wood powder and wood (Rodrigues and Rodrigues, 2017), or poultry manure (Uko et al, 2020) to enhance plant growth. Henceforth, various other substrates could be tested for their use in AMF multiplication.…”

Kajian Jenis Bahan Pembawa Dan Lama Simpan Terhadap Infektivitas Dan Efektivitas Inokulan Fungi Mikoriza Arbuskula

“…Bahan-bahan ini dapat berupa bahan organik dan bahan anorganik atau sintetik (Maria and Felinov, 2017…”