Sporotrichosis With Demonstration of Hypae in Human Tissue

Jd, Maberry; Jf, Mullins; Oj, Stone

doi:10.1001/archderm.1966.01600190071015

Cited by 23 publications

(3 citation statements)

References 7 publications

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“…The carrier liquid containing potassium hydroxide may have dissolved the eosinophilic radiative structures surrounding the fungal cells and make them undetectable. 38 Usually hyphae are very rarely observed in living tissues or exudates from skin lesions, [39][40][41][42] the presence of hyphae seemingly indicates that the agents in living tissues did not produce dimorphism. As yet, this phenomenon does not have a plausible explanation, considering most patients with the presence of hyphae in pus showed open lesions with ulcers and erosions, we speculate that the phenomenon may be attributed to low body surface temperature of patients.…”

Section: Discussionmentioning

confidence: 99%

Retrospective analysis of 71 patients with cutaneous sporotrichosis

Zhu

Xia

Zhi

et al. 2023

Mycoses

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Objective: To summarise 71 cases of cutaneous sporotrichosis in Zhejiang over the past 9 years and analyse clinical and epidemiological characteristics. Methods: This was a retrospective review of patients with cutaneous sporotrichosis attending the Department of Dermatology of the Hangzhou Third People's Hospital between 2013 and 2022. Results:The male-to-female ratio was 1.15:1 among the 71 patients, with a mean age of 55.90 years (±2.02) and an age range of 3 to 94 years. The disease duration was unknown for 17 patients. The intermediate course for the remaining 54 patients lasted 11.90 months, ranging from 1 to 120 months. Thirty-four patients were involved in mixed occupations, 28 were farmers, 4 were housewives, 3 were manufacturing workers, and 2 were carpenters; 23.95% of cases had a history of trauma. The most common clinical manifestation was fixed cutaneous (69.01%), followed by lymphocutaneous (29.58%) and disseminated cutaneous (1.41%). There were 72 affected sites; the upper limbs (69.44%) were affected the most, followed by the face (16.67%) and lower limbs (12.50%). Forty-nine patients showed open lesions (69.01%), 15 showed mixed lesions (21.13%), and seven showed closed lesions (9.86%). Seventy-one patients were confirmed by biopsied tissue or tissue fluid culture. Forty-four patients underwent direct microscopy; of these, 18 (40.91%) were positive and 26 were negative. Molecular analysis confirmed that all fungal strains were Sporothrix globosa. Fiftynine patients underwent histopathological examination, of whom 18 (18.64%) were positive for periodic acid-Schiff (PAS) staining. Eighteen patients were lost to followup; the remaining patients were cured. Conclusions:Consistent with the epidemiological situation of sporotrichosis in other areas of China, S. globosa is the primary pathogen in the Zhejiang province. The primary clinical form of sporotrichosis is fixed cutaneous. Susceptible subjects are mainly middle-aged and elderly rural populations, and males are affected more than females.Patients with cutaneous sporotrichosis do not commonly have a history of obvious trauma. Direct microscopy is important for the diagnosis of sporotrichosis, and itraconazole is a safe and effective treatment option.

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Section: Discussionmentioning

confidence: 99%

Retrospective analysis of 71 patients with cutaneous sporotrichosis

Zhu

Xia

Zhi

et al. 2023

Mycoses

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“…Mycelial hyphae are not usually seen in vivo in biological specimens, in part because of their shortness, their small number, and the difficulty of resolving them from cellular infiltrates, nuclear fragments, and stain particles (90). However, their presence in tissue sections in human sporotrichosis has been reported occasionally (90,103,146). In a biopsy specimen from human tissue which was fixed in 10% formaldehyde, embedded in paraffin, sectioned, and stained in sequence with periodic acid-Schiff reagent and hematoxylin plus eosin, the following fungal elements were detected (90): solitary spores (oval or spherical bodies), a spore with a germ tube, cigar-shaped cells which were free or inside giant cells, asteroid bodies which stained better with hematoxylin plus eosin than with periodic acid-Schiff reagent, and aggregated mycelial hyphae (length, 3 to 15 ,um), which sometimes had a spore attached terminally and stained with periodic acid-Schiff reagent but not with hematoxylin plus eosin.…”

Section: Morphological Diversity and Ultrastructurementioning

confidence: 99%

Sporothrix schenckii and related species of Ceratocystis.

Travassos¹,

Lloyd²

1980

Microbiological Reviews

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“…Usually hyphae are not observed in living tissues, even if their presence has occasionally been demonstrated in bioptical specimens. 2,[6][7][8] We report a case in which we were able to identify hyphae in sputum by means of cytologic techniques.…”

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confidence: 99%