Sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium

Abstract

Two Gram-positive, endospore-forming, rod-shaped bacterial strains, HG645 T and HG711, were respectively isolated from surface water of a brackish lake and sediment of a fishery harbour in Japan and were subsequently characterized taxonomically using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains HG645 T and HG711 are affiliated phylogenetically to the genus Sporosarcina, and they exhibit sequence similarities of 95.7-97.3 % to the type strains of Sporosarcina species. DNA-DNA relatedness between strain HG645 T and the type strain of the phylogenetically related species Sporosarcina aquimarina was less than 10 %. The DNA G+C content of strains HG645 T and HG711 were respectively 46.0 and 45.2 mol%. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell-wall peptidoglycan type (Lys-Glu), major cellular fatty acids (iso-C 15 : 0 and anteiso-C 15 : 0 ) and quinone type (MK-7) of the isolates support their affiliation to the genus Sporosarcina. On the basis of phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent a novel species of the genus Sporosarcina, for which the name Sporosarcina saromensis sp. nov. is proposed. The type strain is strain HG645 T (5MBIC08270 T 5IAM 15429 T 5KCTC 13119 T ).

Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers

“…subtilis ; supplemental and API testing failed to resolve this. By 16S rRNA gene sequencing, its closest match was 98% with the type strain of Sporosarcina saromensis , a relatively new species (An et al. 2007), suggesting this strain may represent a novel taxon.…”

The Prevalence and Control of Bacillus and Related Spore-Forming Bacteria in the Dairy Industry

“…The Sporosarcina genus, established by Kluyver and van Niel (1936) , forms part of the family of the Bacillaceae. At the time of writing, the genus comprised 12 species with Sporosarcina ureae being the type species ( Yoon et al, 2001 ; Reddy et al, 2003 ; An et al, 2007 ; Kwon et al, 2007 ; Yu et al, 2008 ; Tominaga et al, 2009 ; Kämpfer et al, 2010 ; Wolfgang et al, 2012 ). Sporosarcina originate from and are able to thrive in a variety of environments, e.g., soil, seawater, pond water in the Antarctic, human blood, raw bovine milk or factory equipment used in the manufacture of soy sauce ( Yoon et al, 2001 ; Kwon et al, 2007 ; Yu et al, 2008 ; Tominaga et al, 2009 ; Wolfgang et al, 2012 ).…”

Bioconversion of l-arabinose and other carbohydrates from plant cell walls to α-glucan by a soil bacterium, Sporosarcina sp. N52

“…The strain could grow in the presence of NaCl up to 8% (w/v). N52 had a broader range of sugar utilization, optimal growth temperature and pH for growth than previously reported (Kwon et al, 2007;An et al, 2007;Yu et al, 2008;Tominaga et al, 2009). The results of strain identification are summarized in Ta (Claus and Berkeley, 1986).…”