Sporobolomyces linderae, a new ballistosporous anamorphic yeast found on a dead leaf of Lindera obtusiloba collected at Mt. Fuji.

Abstract

A strain of ballistosporous yeast, which was isolated from a dead leaf of Lindera obtusiloba collected at Mt. Fuji, was found to represent a new species. In traditional taxonomic criteria, it resembles Sporobolomyces subbrunneus and Sporobolomyces coprosmicola but showed low DNA relatedness to these two species. The name Sporobolomyces linderae is chosen for this yeast.During the survey on ballistosporous yeasts and yeast-like fungi living on leaves and stems of plants in Japan, 54 ballistosporous strains were isolated from 10 samples of plants collected at Mt. Fuji in 1986 and identified as nine species of yeasts and five species of yeast-like fungi belonging to five genera (9). Five species of yeasts out of nine represented hitherto undescribed species. Four new species, Ballistosporomyces ruber, Ballistosporomyces xanthus, Sporobolomyces yamatoanus and Bullera variabilis, were described for these yeasts (4,7,8). Later, Sp. yamatoanus was transferred to the genus Bensingtonia because of its major ubiquinone, Q-9 (3). The remaining one species is described in this paper as a new species in Sporobolomyces. MATERIALS AND METHODSStrain employed. The strain NB-214 used in the present study was isolated from a dead leaf of Lindera obtusiloba collected at Mt. Fuji by ballistospore-fall method using YM agar plate as described previously (5, 9).Investigation of taxonomic criteria. Most of the methods employed for the examination of morphological, physiological and biochemical characteristics were