Sporobolomyces griseoflavus and Sporobolomyces sasicola, two new species of ballistosporous yeasts isolated from dead leaves of Sasa sp. in Japan.

Abstract

Two new species of ballistospore-forming yeasts, Sporobolomyces griseoflavus and Sporobolomyces sasicola, were isolated from dead leaves of Sasa sp. collected on a mountain in Japan. They form pale colored colonies and bilaterally symmetrical ballistospores, have Q-10 as the major ubiquinone and do not contain xylose in the cells. The taxonomic relationship of these new yeasts to Sporobolomyces singularis are discussed.In the course of investigating ballistospore-forming yeasts living on the surface of various plant materials in Japan, we obtained three strains of hitherto undescribed yeasts from the dead leaves of Sasa sp. collected on mountain in central Japan. These yeasts represent two distinct new species. They are named Sporobolomyces griseoflavus and Sporobolomyces sasicola, and are described in this paper. MATERIALS AND METHODSMicroorganisms. Strains examined were isolated from the dead leaves of Sasa sp. collected on Suga-daira, Nagano Pref., by Dr. G. Okada, in May 1985. These strains were isolated by the ballistospore-fall method of DERx (1) by using YM agar (Difco Labs) plates as described in the previous paper (2).Investigation of taxonomic characteristics. Most of methods used to examine the morphological, physiological, and biochemical characteristics were those described in "The Yeasts, a Taxonomic Study" (3, 4). Assimilation of nitrogen compounds was investigated on solid media with starved inoculum as described in a

The distribution of xylose in the cells of ballistosporous yeasts - Application of high performance liquid chromatography without derivatization to the analysis of xylose in whole cell hydrolysates.

“…gracilis because of a chromatographic noise level, the lack of xylose in the cells of this species was clearly demonstrated by our HPLC method. The type strains of Sporobolomyces oryzicola, Sporobolomyces sasicola, and Sporobolomyces subbrunneus, which were described by us (13,20,21), did not have xylose in their cells. The type strain of Sporobolomyces kluyver-nielii described recently by VAN DER WALT et al (22) did not have xylose in the cells.…”

“…Although the type strain of Sp. sasicola produces pale colored colonies, it is considered to be closely related to typical, deeply pigmented species equipped with Q-10 as the major ubiquinone in the genus Sporobolomyces (21).…”

Plenary lectures

“…(Table 1). Surprisingly, seven species out of ten represented new species though the frequency of isolation of each species is not high (Nakase & Suzuki, 1985, 1986bNakase et al, 1987b). In contrast to the report of Yamada et al (1973Yamada et al ( , 1983, five of them have Q-9 as the major ubiquinone.…”

Bullera variabilis, a new species of yeast with uniquely-sharped ballistospores isolated from various plant materials.

“…Acetone-dried cells harvested in the logarithmic growth phase were hydrolyzed and neutralized as described in an earlier paper (9). Xylose in cell hydrolyzates was analyzed by high performance liquid chromatography using a Shodex SP 1010 column (Showa Denko) with water as the mobile phase at a flow rate of 0.8 ml/min at 80°C, and a Shodex RS pak DC-613 column with acetonitrile-water (80: 20, v/v) as the mobile phase at a flow rate of 0.8 ml/min at 75 C. A refractive index monitor was used to detect carbohydrates.…”