SPORE FORMATION BY <i>CLOSTRIDIUM</i> SPECIES IN AN ARTIFICIAL MEDIUM

Gibbs, B. M.; Hirsch, A.

doi:10.1111/j.1365-2672.1956.tb00056.x

Cited by 44 publications

(17 citation statements)

References 18 publications

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“…The modern cold catalyst version (Heller, 1954) of the classical anaerobic jar (Baird and Tatlock Ltd., London) gives good results 1 0 5 with anaerobic plate cultures and it is much easier to use than the original jar. Of the many methods which used pyrogallol to absorb the oxygen since Buchner's original invention (1888), the more recent include those of Mossel, Golstein Brouwers & de Bruin (1959), Gibbs & Hirsch (1956) and Matthew & Karnauchow (1961). I n our experience these give exccllent results with most strains but fail inexplicably a t times and we no longer use these routinely.…”

Section: (C) Methods Of Obtaining Anuerobiosismentioning

confidence: 99%

“…Crystal violet, used a long time ago (Churchman, 1912;Hall & Ellefson, 1918)) has also been reconsidered recently (Blenden & Merilan, 1961;Gibbs & Freame, 1962). The use of O-ooOo5% of crystal violet a t pH 8 made RCM (Reinforced Clostridial Medium, Hirsch & Grinstead, 1954;Gibbs & Hirsch, 1956) selective for clostridia against most aerobic spores when mixed cultures were tested in the laboratory, but it failed with natural samples (Gibbs & Freame, 1962). Blenden & Merilan (1962) reported good results with crystal violet a t a concentration of 1 : 750,000 (0~00013~0) in solid media.…”

Section: Culture Mediamentioning

confidence: 99%

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Methods for the Recovery of Clostridia from Foods

Gibbs

Freame

1965

Journal of Applied Bacteriology

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Section: (C) Methods Of Obtaining Anuerobiosismentioning

confidence: 99%

Section: Culture Mediamentioning

confidence: 99%

Methods for the Recovery of Clostridia from Foods

Gibbs

Freame

1965

Journal of Applied Bacteriology

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“…(a) Preliminary investigations As shown above, it does not appear possible in a single medium to suppress the food streptococci without inhibiting the clostridia. The usual pasteurization method of eliminating such interfering organisms (Klein, 1895 ;Weinzirl, 1921) is also unsuitable; it may result in the killing of over 99% of certain clostridia (Ingram & Hauge, 1948;Hobbs et al 1953;Saleh & Ordal, 1955;Gibbs & Hirsch, 1956). Accordingly, a differential medium of the sulphite-iron agar type (Wilson & Blair, 1924) was next tested.…”

Section: The Use Of Sulphite-iron Aammentioning

confidence: 99%

THE ENUMERATION OF ANAEROBIC BACTERIA, AND OF CLOSTRIDIUM SPECIES IN PARTICULAR, IN FOODS

Mossel¹,

Bruin²,

Diepen³

et al. 1956

Journal of Applied Bacteriology

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“…wekchii in various special media (Gibbs and Hirsch, 1956;Ellner, 1956). Rapid and abundant sporulation of Cl.…”

Section: Discussionmentioning

confidence: 99%

Free-sporing Cl. welchii in ordinary laboratory media and conditions.

Rahman¹

1978

J Clin Pathol

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SUMMARY A strain of Clostridium welchii produced spores in ordinary blood agar plates. Investigations confirmed that it was the character of this particular strain and that the laboratory media were not inducing sporulation. During a period of 12 months a total of 100 strains of Cl. welchii were studied. None of them produced spores in ordinary laboratory media and conditions when examined microscopically.The general statement that Clostridium welchii produces spores in the gut but not in wounds or in routine media applies mainly to type A, which is chiefly associated with human disease (Willis, 1969 To check for any agent in the laboratory media that might have stimulated spore formation a few other strains of Cl. welchii were grown in parallel with the original strain (strain 0) in identical conditions of media, temperature, and time. Ten different strains of Cl. welchii were isolated from different stool specimens, which were selected randomly without knowing the clinical condition of the patients from whom specimens were received. All these new strains and strain 0 were then subcultured on to blood agar plates, incubated anaerobically at 370C overnight, and examined in the morning. Selected colonies from each culture were then Gram-stained and stained for spores. ResultsFive colonies from the culture plate given in the class showed Gram-positive rods. Many of them had unstained oval areas, most of which were situated subterminally. Spore staining showed spores as green oval areas on the subterminal or terminal positions of red-stained rods. Staining of a few more subcultures from these colonies showed similar spores. When incubation was continued up to the second or the third day abundant free spores 359 on 8 May 2018 by guest. Protected by copyright.

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SPORE FORMATION BY CLOSTRIDIUM SPECIES IN AN ARTIFICIAL MEDIUM

Cited by 44 publications

References 18 publications

Methods for the Recovery of Clostridia from Foods

Methods for the Recovery of Clostridia from Foods

THE ENUMERATION OF ANAEROBIC BACTERIA, AND OF CLOSTRIDIUM SPECIES IN PARTICULAR, IN FOODS

Free-sporing Cl. welchii in ordinary laboratory media and conditions.

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