Spontaneously occurring fundus findings observed using confocal scanning laser ophthalmoscopy in wild type Sprague Dawley rats

Joshi, Rahul; Pankova, Natalie; Wang, Hai; Baek, David Sung Hyeon; Zhao, Xu; Reyad, Matthew; Boyd, Shelley

doi:10.1016/j.yrtph.2016.02.002

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…SD-OCT provides information of retinal structure and retinal thickness by collecting backscattering data to create cross-sectional images of the retina, while SLO collects fluorescence data to produce stereoscopic high-contrast images of the retina. Nowadays, both techniques are increasingly used in experimental ophthalmology using small rodents 12,13,14,15 (or even zebrafish 16,17 ) and can provide both qualitative and quantitative information 12,17,18,19,20,21 .…”

Section: Introductionmentioning

confidence: 99%

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Kokona¹,

Jovanovic²,

Ebneter³

et al. 2017

JoVE

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Spectral domain optical coherence tomography (SD-OCT) and scanning laser ophthalmoscopy (SLO) are extensively used in experimental ophthalmology. In the present protocol, mice expressing green fluorescent protein (gfp) under the promoter of Cx3cr1 (BALB/c-Cx3cr1) were used to image microglia cells in vivo in the retina. Microglia are resident macrophages of the retina and have been implicated in several retinal diseases. This protocol provides a detailed approach for generation of retinal B-scans, with SD-OCT, and imaging of microglia cell distribution in Cx3cr1 mice with SLO in vivo, using an ophthalmic imaging platform system. The protocol can be used in several reporter mouse lines. However, there are some limitations to the protocol presented here. First, both SLO and SD-OCT, when used in the high-resolution mode, collect data with high axial resolution but the lateral resolution is lower (3.5 µm and 6 µm, respectively). Moreover, the focus and saturation level in SLO is highly dependent on parameter selection and correct alignment of the eye. Additionally, using devices designed for human patients in mice is challenging due to the higher total optical power of the mouse eye compared to the human eye; this can lead to lateral magnification inaccuracies, which are also dependent on the magnification by the mouse lens among others. However, despite that the axial scan position is dependent upon lateral magnification, the axial SD-OCT measurements are accurate.

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Section: Introductionmentioning

confidence: 99%

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Kokona¹,

Jovanovic²,

Ebneter³

et al. 2017

JoVE

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“…Both instruments enable non-invasive, in vivo assessment of ocular tissues from a multitude of animal species. In vivo assessments have previously demonstrated that abnormal phenotypes may arise in both mammals and zebrafish that are present prior to the initiation of experimental studies (Bell et al, 2015; Bell et al, 2012; Joshi et al, 2016; Mattapallil et al, 2012; Tschopp et al, 2010). Using these instruments to screen for baseline abnormalities will avoid the potential for unexpected, confusing and confounding experimental results.…”

Section: Introductionmentioning

confidence: 99%

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Bell

Yuan

DiCicco

et al. 2016

Experimental Eye Research

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Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We utilized these two independent imaging modalities in a complementary manner to perform in vivo optical sectioning of the adult zebrafish retina. Due to the very high optical power of the zebrafish lens, the confocal depth of field is narrow, allowing for detailed en face views of specific retinal layers, including the cone mosaic. Moreover, we demonstrate that both native reflectance, as well as fluorescent features observed by SLO, can be combined with axial in-depth information obtained by SDOCT. These imaging approaches can be used to screen for ocular phenotypes and monitor retinal pathology in a non-invasive manner.

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Evolving Patterns of Hyperfluorescent Fundus Autofluorescence Accompany Retinal Atrophy in the Rat and Mimic Atrophic Age-Related Macular Degeneration

Pankova

Baek

Zhao

et al. 2022

Trans. Vis. Sci. Tech.

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Spontaneously occurring fundus findings observed using confocal scanning laser ophthalmoscopy in wild type Sprague Dawley rats

Cited by 3 publications

References 22 publications

<em>In Vivo</em> Imaging of <em>Cx3cr1<sup>gfp/gfp</sup></em> Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

<em>In Vivo</em> Imaging of <em>Cx3cr1<sup>gfp/gfp</sup></em> Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Evolving Patterns of Hyperfluorescent Fundus Autofluorescence Accompany Retinal Atrophy in the Rat and Mimic Atrophic Age-Related Macular Degeneration

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