Spontaneous occurrence of photoageing‐like phenotypes in the dorsal skin of old <scp>SAMP</scp>1 mice, an oxidative stress model

Sakura, Masaaki; Chiba, Yoichi; Kamiya, Emi; Furukawa, Ayako; Kawamura, Noriko; Niwa, Masanao; Takeuchi, Minoru; Hosokawa, Masanori

doi:10.1111/exd.12059

Cited by 10 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies have tested the effects of solar radiation and oxidative stress on the skin [29, 83–85], and oxidative stress has been linked to age-related loss of skin elasticity [86–88], defective cellular signaling [68] and photoaging [89, 90]. Because it triggers cellular damage pathways, oxidative stress activates cellular senescence which is thought to directly lead to photoaging [91–94]. Cellular senescence is associated with a reduced capacity to divide and proliferate, sometimes in conjunction with shortening of telomeres [95–98].…”

Section: Oxidative Stress and Agingmentioning

confidence: 99%

Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation

2014

View full text Add to dashboard Cite

Being the largest and most visible organ of the body and heavily influenced by environmental factors, skin is ideal to study long-term effects of aging. Throughout our lifetime, we accumulate damage generated by UV radiation. UV causes inflammation, immune changes, physical changes, impaired wound healing and DNA damage that promotes cellular senescence and carcinogenesis. Melanoma is the deadliest form of skin cancer and among the malignancies of highest increasing incidence over the last several decades. Melanoma incidence is directly related to age, with highest rates in individuals over the age of 55 years, making it a clear age-related disease. In this review, we will focus on UV-induced carcinogenesis and photo aging along with natural protective mechanisms that reduce amount of “realized” solar radiation dose and UV-induced injury. We will focus on the theoretical use of forskolin, a plant-derived pharmacologically active compound to protect the skin against UV injury and prevent aging symptoms by up-regulating melanin production. We will discuss its use as a topically-applied root-derived formulation of the Plectranthus barbatus (Coleus forskolii) plant that grows naturally in Asia and that has long been used in various Aryuvedic teas and therapeutic preparations.

show abstract

Section: Oxidative Stress and Agingmentioning

confidence: 99%

Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation

2014

View full text Add to dashboard Cite

show abstract

“…A senescence-accelerated mouse (SAM) was generated by selective inbreeding of AKR/J strain, resulting in the establishment of 9 strains of senescence-prone (SAMP) mice and 3 senescence-resistant (SAMR) mice with strain-unique phenotypes of accelerated aging [28]. In the skin, SAMP1 strain is reported to exhibit an increase in elastic fibers and epidermal thickness at 1 to 1.5 year of age [29], which resemble characteristics of human photoaging, a skin damage resulting from prolonged sunlight exposure [30, 31]. SAMP8 strain shows neurodegenerative and cognitive deficits associated with Alzheimer’s disease, and abnormality in circadian rhythms along with a shorter lifespan [32–34].…”

Section: Introductionmentioning

confidence: 99%

Wild-type and SAMP8 mice show age-dependent changes in distinct stem cell compartments of the interfollicular epidermis

et al. 2019

View full text Add to dashboard Cite

Delayed wound healing and reduced barrier function with an increased risk of cancer are characteristics of aged skin and one possible mechanism is misregulation or dysfunction of epidermal stem cells during aging. Recent studies have identified heterogeneous stem cell populations within the mouse interfollicular epidermis that are defined by territorial distribution and cell division frequency; however, it is unknown whether the individual stem cell populations undergo distinct aging processes. Here we provide comprehensive characterization of age-related changes in the mouse epidermis within the specific territories of slow-cycling and fast-dividing stem cells using old wild-type, senescence-accelerated mouse prone 1 (SAMP1) and SAMP8 mice. During aging, the epidermis exhibits structural changes such as irregular micro-undulations and overall thinning of the tissue. We also find that, in the old epidermis, proliferation is preferentially decreased in the region where fast-dividing stem cells reside whereas the lineage differentiation marker appears to be more affected in the slow-cycling stem cell region. Furthermore, SAMP8, but not SAMP1, exhibits precocious aging similar to that of aged wild-type mice, suggesting a potential use of this model for aging study of the epidermis and its stem cells. Taken together, our study reveals distinct aging processes governing the two epidermal stem cell populations and suggests a potential mechanism in differential responses of compartmentalized stem cells and their niches to aging.

show abstract

“…The changes included an increase in elastic fibers and GAGs, an up-regulation of several pro-inflammatory cytokines and MMPs, and an increase in lipid peroxide in the dorsal skin. In contrast, SAMR1 mice did not exhibit such skin changes at an older age [21]. On the basis of these findings, we have proposed that aggravated intrinsic mechanisms, i.e., higher oxidative status, might induce photoaging-like phenotypes in SAMP1 mice.…”

Section: Introductionmentioning

confidence: 72%

“…To confirm the association between the skin photoaging phenotype and the shift to pro-inflammatory status, we evaluated the IFN-γ/IL-4 ratio in SAMP1 mice, another skin photoaging model [21]. The IFN-γ/IL-4 ratio of the skin from 70-week-old SAMP1 mice was markedly increased as compared to that of 12-week-old SAMP1 mouse skin (6.64 ± 2.44 and 0.06 ± 0.03, respectively: p = 0.0038, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…We have recently reported a spontaneous animal model for photoaging, probably caused by an exaggerated intrinsic mechanism [21]. Accelerated senescence-prone (SAMP) mice show an accelerated senescence process, shorter lifespan, higher oxidative status, and an earlier onset and more rapid progression of age-associated pathological phenotypes as compared to accelerated senescence-resistant (SAMR) mice, a closely related control strain which shows a normal aging process [22]- [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differences in the Histopathology and Cytokine Expression Pattern between Chronological Aging and Photoaging of Hairless Mice Skin

Sakura¹,

Chiba²,

Kamiya³

et al. 2014

MRI

Self Cite

View full text Add to dashboard Cite

Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated hairless mice have been used as a skin photoaging animal model. However, differences in morphology and gene expression patterns between UV-induced and chronological skin changes in this mouse model have not been fully elucidated. Here we investigated differences in histopathology and cytokine expression between UV-irradiated and non-irradiated aged hairless mice to clarify the factor(s) that differentiate photoaging from chronological skin aging phenotypes. Eight-week-old HR-1 hairless mice were divided into UV-irradiated (UV-irradiated mice) and non-irradiated (control mice) groups. Irradiation was performed three times per week for 10 weeks. In addition, 30-week-old HR-1 hairless mice were reared until 70 weeks of age without UV irradiation (aged mice). Histopathologies revealed that the flattening of dermal-epidermal junctions and epidermal thickening were observed only in UV-irradiated mice. Decreases in fine elastic fibers just beneath the epidermis, the thickening of elastic fibers in the reticular dermis, and the accumulation of glycosaminoglycans were more prominent in UV-irradiated mice as compared to non-irradiated aged mice. Quantitative PCR analyses revealed that UV-irradiated mice showed * Corresponding author. M. Sakura et al. 83 an increase in the expression of IFN-γ. In contrast, aged mice exhibited proportional up-regulation of both pro-inflammatory and anti-inflammatory cytokines. The IFN-γ/IL-4 ratio, an indicator for the balance of pro-inflammatory and anti-inflammatory cytokines, was significantly higher in UVirradiated mice as compared to control and non-irradiated aged mice. An elevated IFN-γ/IL-4 ratio was also observed in aged senescence-accelerated mouse-prone 1 (SAMP1) mice, a spontaneous skin photoaging model we recently reported. Thus, an imbalance between pro-inflammatory and anti-inflammatory cytokines might be a key factor to differentiate photoaged skin from chronologically-aged skin.

show abstract

Spontaneous occurrence of photoageing‐like phenotypes in the dorsal skin of old SAMP1 mice, an oxidative stress model

Cited by 10 publications

References 28 publications

Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation

Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation

Wild-type and SAMP8 mice show age-dependent changes in distinct stem cell compartments of the interfollicular epidermis

Differences in the Histopathology and Cytokine Expression Pattern between Chronological Aging and Photoaging of Hairless Mice Skin

Contact Info

Product

Resources

About