Spontaneous micronuclei in peripheral blood erythrocytes from 35 mammalian species

G, Zúñiga; Bugarín, Olivia Torres; Ramı́rez-Muñoz, M.P; Ramos, Anselmo Silva; Fanti-Rodriguez, E.; Portilla, Eliana; García‐Martínez, Daniel; Cantú, J. M.; Gallegos‐Arreola, Martha Patricia; Sánchez‐Corona, José

doi:10.1016/s0165-1218(96)90056-7

Cited by 36 publications

(27 citation statements)

References 11 publications

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“…Since 1996, our group has worked on identifying animal species with measurable frequencies of spontaneous micronucleated erythrocytes (MNEs) circulating in their peripheral blood [Zúñiga et al, 1996a;Zúñiga-González et al, 2000;Zúñiga-González et al, 2001a;Zúñiga-González et al, 2002], with the idea that analysis of MNE frequencies in these animals can be used as a biomonitor of exposure to genotoxic agents [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b;Gómez-Meda et al, 2004]. A highly proliferating tissue like blood is required to carry out this assay [Lähdetie, 1983;Shuilin and Baker, 1989;Schmezer et al, 1990;Torres-Bugarín et al, 1998;Zamora-Perez et al, 2004], and only a drop of blood is needed to measure micronucleus (MN) frequencies in peripheral blood, so that it is not necessary to kill the animal [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b].…”

Section: Introductionmentioning

confidence: 99%

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2005

Environ. Mol. Mutagen.

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Nonhuman primates are of particular relevance in evaluating the potential toxicity of drugs and environmental agents. We have used previously published information and data from the present study to establish a relationship for New World (NW) and Old World (OW) primates on the basis of the frequency of spontaneous micronucleated erythrocytes (MNEs) observed in peripheral blood. Data on spontaneous MNEs in peripheral blood from 15 species of primates, including humans, indicate that NW primates have significantly (P < 0.01) higher MNE frequencies (group mean, 9.5 +/- 7.3 MNEs/10,000 erythrocytes; range, 0.7-20.5/10,000 erythrocytes) than OW primates (group mean, 1.0 +/- 0.9 MNEs/10,000 erythrocytes; range, 0.0-2.6 MNEs/10,000 erythrocytes). Humans are believed to have developed in the OW, and human MNE frequencies were similar to those described for OW primate species. We selected the common marmoset (Callithrix jacchus), a NW primate, to determine whether therapeutic pediatric doses of Metotrexate (MTX; 2.5 mg/kg), Cyclophosphamide (CP; 5 mg/kg), Cytosine-arabinoside (Ara-C; 3 mg/kg), or 5-Fluorouracil (5-FU; 10 mg/kg), administered daily for two consecutive days, increase the frequency of micronuclei. Micronucleated polychromatic erythrocyte frequencies were increased significantly in groups receiving MTX, CP and Ara-C, while MNE frequencies were increased by the Ara-C treatment. The results of this study indicate that NW primates have higher spontaneous MNE frequencies than OW primates, and because of this, NW primates like the common marmoset, may be suitable for evaluating the genotoxicity of chemical agents.

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Section: Introductionmentioning

confidence: 99%

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2005

Environ. Mol. Mutagen.

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“…The microbiological analyses carried out at the water entry and exit points in the zoo detected the presence of total and fecal coliforms (Table 4) Table 2. ANOVA results on number of micronuclei in bird and mammal species studied and those reported by Zúñiga et al (1996) and Zúñiga-González et al (2000;2001). impact among the species studied.…”

Section: Discussionmentioning

confidence: 65%

“…In both cases, the differences in genotoxic responses may reside in the inherent biology of these species submitted to similar selective pressure (Zúñiga et al 1996;Meier et al 1999;Ferraro et al 2004;Newman 2001). Analyzing the expression of micronucleated cells in intra-species terms, Mazama gouazoubira achieved the lowest mean values of micronucleated cells (Tables 1 and 3), which may be related to the low metabolic rate of the species in artificial environments.…”

Section: Speciesmentioning

confidence: 97%

“…Inherent metabolic rates on the individual or species level may result in a different expression of micronuclei (Meier et al 1999). Further evidence for such a hypothesis is found in the different micronucleus frequencies in the peripheral blood samples among 35 different mammal species (Zúñiga et al 1996).…”

Section: Speciesmentioning

confidence: 99%

“…Frequencies were compared by using one-way analysis of variance (ANOVA) with specific basal and normal-like frequency (control value) of micronuclei found in the literature, according to Zúñiga et al (1996) and Zúñiga-González et al 2000;2001. The criteria used for defining the reference groups were the close phylogenetic relationships and shared or at least similar habits regarding feeding, behavior, metabolism and habitat.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Adam¹,

Torres²,

Kiska³

et al. 2013

NatCon

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Ex-situ conservation at zoos requires strategies regarding improvements to enclosures, as ex-situ conserved specimens constitute an effective reservoir for genetic and biological restoration. The present study focuses on the management of enclosures at zoos and offering an accurate, fast and inexpensive procedure for ex-situ conservation through the assessment of the degree of genome damage (micronuclei) in different bird and mammal species. Analyses of variance revealed statistically significant differences in both bird and mammal species, in comparison to their reference groups. All bird species and Tapirus terrestris had high proportional micronucleus indexes, indicating high susceptibility to genome damage through contact with polluted water from the river used to supply the enclosures. Recognizing the relationship between water quality (evaluated at entry and exit of water in the zoo) and habitat deterioration as well as individual responses to these factors in terms of genotoxicity make efforts directed at ex-situ conservation an urgent priority.

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Feasibility of micronucleus methods for monitoring genetic damage in two feral species of small mammals

Meier

Wernsing

Torsella³

1999

Environ. Mol. Mutagen.

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Spontaneous micronuclei in peripheral blood erythrocytes from 35 mammalian species

Cited by 36 publications

References 11 publications

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Feasibility of micronucleus methods for monitoring genetic damage in two feral species of small mammals

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