Feasibility of micronucleus methods for monitoring genetic damage in two feral species of small mammals

Meier, John R.; Wernsing, Paul; Torsella, Joni

doi:10.1002/(sici)1098-2280(1999)33:3<219::aid-em6>3.0.co;2-x

Cited by 18 publications

(8 citation statements)

References 20 publications

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“…Despite of its widespread use in the evaluation of contaminants, the micronucleus test has received minimal attention in genotoxic evaluations of in-situ and ex-situ conservation issues (Meier et al 1999). Two seminal studies have been carried out on captive species (domestic) in order to evaluate putative DNA damage (micronuclei frequency) through intense medicinal administrations (Cristaldi et al 2004;Adam et al 2005).…”

Section: Resultsmentioning

confidence: 99%

“…In both cases, the differences in genotoxic responses may reside in the inherent biology of these species submitted to similar selective pressure (Zúñiga et al 1996;Meier et al 1999;Ferraro et al 2004;Newman 2001). Analyzing the expression of micronucleated cells in intra-species terms, Mazama gouazoubira achieved the lowest mean values of micronucleated cells (Tables 1 and 3), which may be related to the low metabolic rate of the species in artificial environments.…”

Section: Speciesmentioning

confidence: 97%

“…Such a result could be the consequence of an increase in metabolic oxiradicals caused by high metabolic rates. Inherent metabolic rates on the individual or species level may result in a different expression of micronuclei (Meier et al 1999). Further evidence for such a hypothesis is found in the different micronucleus frequencies in the peripheral blood samples among 35 different mammal species (Zúñiga et al 1996).…”

Section: Speciesmentioning

confidence: 99%

“…Studies should also be carried out on cellular kinetics in the erythrocytes of birds and mechanisms regarding the exclusion of micronucleated cells in the blood of both biological groups. According to Meier et al (1999) and Grisolia and Cordeiro (2000), low frequencies of micronucleated erythrocytes may be an indication of their removal from the circulating blood by the spleen. Such studies would be important in terms of a better identification of more susceptible species as well as more effective bioindication.…”

Section: Fecal Totalmentioning

confidence: 99%

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Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Adam¹,

Torres²,

Kiska³

et al. 2013

NatCon

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Ex-situ conservation at zoos requires strategies regarding improvements to enclosures, as ex-situ conserved specimens constitute an effective reservoir for genetic and biological restoration. The present study focuses on the management of enclosures at zoos and offering an accurate, fast and inexpensive procedure for ex-situ conservation through the assessment of the degree of genome damage (micronuclei) in different bird and mammal species. Analyses of variance revealed statistically significant differences in both bird and mammal species, in comparison to their reference groups. All bird species and Tapirus terrestris had high proportional micronucleus indexes, indicating high susceptibility to genome damage through contact with polluted water from the river used to supply the enclosures. Recognizing the relationship between water quality (evaluated at entry and exit of water in the zoo) and habitat deterioration as well as individual responses to these factors in terms of genotoxicity make efforts directed at ex-situ conservation an urgent priority.

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Section: Resultsmentioning

confidence: 99%

Section: Speciesmentioning

confidence: 97%

Section: Speciesmentioning

confidence: 99%

Section: Fecal Totalmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Adam¹,

Torres²,

Kiska³

et al. 2013

NatCon

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show abstract

“…The idea that different responses to genotoxic agents may reflect intrinsic differences rather than differences related to exposure is corroborated (21). The presence or absence of certain species in an environment may often be related to severe effects of environmental stressors, which lead to the elimination of one or more species from certain localities.…”

Section: Discussionmentioning

confidence: 99%

Environmental Genotoxicity Evaluation Using Cytogenetic End Points in Wild Rodents

Bueno

Pereira

Rabello-Gay

2000

Environmental Health Perspectives

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The analysis of cytogenetic end points in organisms exposed to chemicals in their natural environment may help in the early detection of genotoxic damage. This methodology was used to evaluate the impact of environmental compounds occurring in the Itajaí Valley, state of Santa Catarina, Southern Brazil. The lumber, textiles, paper, cigarette, and pesticide industries, besides intense rice cultivation, are the main economic sources of the region. Three areas were chosen for this study: an industrial, an agricultural, and a permanent preservation area. Two species of wild rodents naturally occurring in the three areas, Akodon montensis and Oryzomys nigripes, were analyzed.Cytogenetic preparations were obtained using conventional methods. The end points under study were the polychromatic/normochromatic cell ratio (PCE/NCE); the mitotic index (MI); the frequency of cells with micronuclei in the bone marrow and peripheral blood; and the frequency of cells with chromosome aberrations in the bone marrow.The purpose of the study was to determine the performance of the species and cytogenetic end points as indicators of genotoxic activity in the study areas. The results show a) the role of the end points used as biomarkers in the early detection of genotoxic agents and in the identification of species and populations at higher risk; b) the difference in sensitivity of the species selected as bioindicators in relation to the cytogenetic end points analyzed; c) the need to use several end points when trying to establish a genotoxic profile of an area; and d) the need to use at least two sympatric species to detect the presence of genotoxins in each locality. Materials and MethodsCollecting areas. The area of study is located at the Medium Valley of the Itajaí River, state of Santa Catarina, Brazil. We selected three collecting points: a preservation area, to monitor animals not directly exposed to environmental pollution and for comparison with the other two points; a rice field, to evaluate the genotoxic effects of pesticides used in the rice culture; and an industrial area, to assess the effect of industrial, domestic, and agricultural waste.Animals. Because there were no previous records on the diversity and density of wild rodents in the region, we chose the species for this study after the captures. Three criteria were established: range of distribution, including the three selected locals; population density (enough to ensure a sample of approximately 10 animals of each species at each collecting point during a 1-year period); and sympatry.Based on these criteria and on the results of the captures, two species of wild rodents were chosen: Akodon montensis (2n = 24) and Oryzomys nigripes (2n = 62), both well-known cytogenetically. Individuals were live-trapped at the three collecting areas.Cytogenetic preparations and analysis. Once in the laboratory, the animals were weighed, sexed, and marked. One hour after being injected with a 0.01% colchicine solution (1 mL/100 g/body weight), they were killed by ether inhalatio...

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Feasibility of conducting the micronucleus test in circulating erythrocytes from different mammalian species: An anatomical perspective

Udroiu

2006

Environ and Mol Mutagen

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The in vivo mammalian micronucleus test can be conducted easily on peripheral blood samples since the maturation of erythrocytes involves the loss of the major nucleus. In addition, mature erythrocytes are relatively long-lived, so that the test potentially can detect genotoxic damage caused by chronic exposures. However, some species have spleens that remove micronuclei from the peripheral circulation, making such measurements problematical. This report summarises haematological and mutagenesis studies dealing with this subject and provides an anatomical interpretation of the phenomenon. Anatomical features can be used to identify those species in which micronuclei are removed by the spleen.

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Feasibility of micronucleus methods for monitoring genetic damage in two feral species of small mammals

Cited by 18 publications

References 20 publications

Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Assessment of Genome Damage in Bird and Mammal Species as a Tool for Improvements in ex-situ Conservation at Zoos

Environmental Genotoxicity Evaluation Using Cytogenetic End Points in Wild Rodents

Feasibility of conducting the micronucleus test in circulating erythrocytes from different mammalian species: An anatomical perspective

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