Spontaneous in vitro contractile activity of specimens from the abomasal wall of healthy cows and comparison among dairy breeds

Zulauf, Marc; Spring, C.; Eicher, R; Meylan, Mireille; Hirsbrunner, Gabriela; Scholtysik, Gfinter; Steiner, Adrian

doi:10.2460/ajvr.2002.63.1687

Cited by 7 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of the preparations from the abomasal antrum exhibited spontaneous contractility, which is in accordance with the results of the study on physiological contractility patterns of the abomasal antrum (Zulauf et al, 2002). Spontaneous motility was demonstrated in approximately 75% of the specimens from abomasal antrum in vitro and a wide variability of the spontaneous activity patterns of the preparations was found (Zulauf et al, 2002). The present experiments revealed that neither CIS nor MET evoked a significant effect on smooth muscle preparations from duodenum and abomasal antrum of dairy cows under the experimental conditions used in this study.…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

“…Both CIS and MET did not reveal any significant effect on contractility of smooth muscle preparations from the bovine abomasal antrum and duodenum. The majority of the preparations from the abomasal antrum exhibited spontaneous contractility, which is in accordance with the results of the study on physiological contractility patterns of the abomasal antrum (Zulauf et al, 2002). Spontaneous motility was demonstrated in approximately 75% of the specimens from abomasal antrum in vitro and a wide variability of the spontaneous activity patterns of the preparations was found (Zulauf et al, 2002).…”

Section: Discussionsupporting

confidence: 88%

“…, 2002). Spontaneous motility was demonstrated in approximately 75% of the specimens from abomasal antrum in vitro and a wide variability of the spontaneous activity patterns of the preparations was found (Zulauf et al. , 2002).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

In vitroeffects of cisapride, metoclopramide and bethanechol on smooth muscle preparations from abomasal antrum and duodenum of dairy cows

Michel¹,

Mevissen

Burkhardt³

et al. 2003

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

The objective of this study was to investigate the effects of cisapride (CIS), metoclopramide (MET) and bethanechol (BET) on contractility parameters from smooth muscle preparations of the abomasal antrum and proximal duodenum of cows. Smooth muscle preparations were harvested shortly post-mortem from 42 healthy dairy cows, and concentration-response curves were performed by cumulative application of the drugs. Cisapride and MET did not have any significant effect on the contractility parameters studied, while BET induced a significant, concentration-dependent increase in basal tone (BT), mean amplitude (Amean), and area under the curve (AUC) in smooth muscle preparations from the abomasal antrum, but not from the duodenum. The effect of BET on BT was more pronounced in specimens with longitudinal orientation while the maximal obtainable effect (Vm) in Amean was more pronounced in circular-oriented preparations. Atropine (1 x 10-5 m) significantly inhibited the effect of BET, whereas pre-incubation with hexamethonium or tetrodotoxin (TTX) had no effect, suggesting that the effect was mediated by cholinergic receptors on the smooth muscle. The results may be relevant to diseases or disorders associated with gastric emptying and gastric hypomotility. Further investigations are warranted to investigate the potential ability of BET to enhance abomasal emptying of adult dairy cows.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

In vitroeffects of cisapride, metoclopramide and bethanechol on smooth muscle preparations from abomasal antrum and duodenum of dairy cows

Michel¹,

Mevissen

Burkhardt³

et al. 2003

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

show abstract

“…under isometric conditions. The contraction activities, amplitudes, and frequencies that were measured and calculated were in the same order of magnitude as those reported in previous experiments with circular muscles from the abomasal corpus of cows (Zulauf et al, 2002).…”

Section: Figuresupporting

confidence: 61%

Potassium and insulin affect the contractility of abomasal smooth muscle

Türck

Leonhard-Marek

2010

Journal of Dairy Science

View full text Add to dashboard Cite

Abomasal displacement is a frequent and important disease of high yielding dairy cows. Although several factors are related to its occurrence, the pathogenesis of the condition is still inadequately understood, particularly in regard to K(+) and insulin homeostasis. For this reason the aim was to investigate the effects of K(+) and insulin concentrations on in vitro motility of abomasal smooth muscle. The second aim was to determine whether the in vivo change in K(+) and insulin levels might be sufficient to induce reduced abomasal motility. Muscle strips were isolated from the abomasum of slaughtered cows and incubated in buffer solution under isometric conditions. Results show that a decrease in extracellular K(+) (between 5 and 1 mmol/L) or an increase in extracellular insulin concentrations (to 21 mU/L or higher) were able to affect the contraction activity of abomasal muscles. Contraction activity given as median (25th, 75th percentiles) changed from 28.1 mN/min (2.5, 49.9) at 5 mmol/L of K(+) to 9.4 mN/min (0.6, 35.7) at 1 mmol/L of K(+), and from 34.5 mN/min (10.8, 112.4) at 0 mU/L of insulin to 12.0 mN/min (7.6, 49.8) at 120 mU/L of insulin. Because the effect of insulin could be abolished by barium, glybenclamide, or ouabain, the underlying mechanisms of the insulin action could be an increased K(+) conductance or an increased Na/K-ATPase activity or both. Low K(+) or high insulin concentrations both reduced the activity of the circular muscle of the abomasal corpus (i.e., of the part that is responsible for the propulsion of abomasal chymus) and might play an important role in the pathogenesis of abomasal displacement.

show abstract

Evaluation of biochemical parameters for the prediction of left displaced abomasum in dairy cows using the logistic regression model

et al. 2012

View full text Add to dashboard Cite

Spontaneous in vitro contractile activity of specimens from the abomasal wall of healthy cows and comparison among dairy breeds

Cited by 7 publications

References 17 publications

In vitroeffects of cisapride, metoclopramide and bethanechol on smooth muscle preparations from abomasal antrum and duodenum of dairy cows

In vitroeffects of cisapride, metoclopramide and bethanechol on smooth muscle preparations from abomasal antrum and duodenum of dairy cows

Potassium and insulin affect the contractility of abomasal smooth muscle

Evaluation of biochemical parameters for the prediction of left displaced abomasum in dairy cows using the logistic regression model

Contact Info

Product

Resources

About