Spontaneous hybridization of the putative ancestors of the allotetraploid <i>Poa annua</i>

Darmency, Henri; Gasquez, Jacques

doi:10.1046/j.1469-8137.1997.00772.x

Cited by 21 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here we confirm previous results revealing a hybrid origin of the annual bluegrass ( P. annua ) from the two diploids P. supina and P. infirma (Tutin , 1957, Chrtek and Jirásek 1962, Darmency and Gasquez , Soreng et al , Mao and Huff ). This origin from two known species was slightly doubted by Koshy (1968) who found morphological dissimilarities in between the chromosome set of the allotetraploid P. annua in and its putative parental species, but all recent data on P. annua indicate that it has underwent post‐hybridization modification of the karyotype, an alternative also considered by Koshy (1968).…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

On polyphyly of the former section Ochlopoa and the hybridogenic section Acroleucae (Poa , Poaceae): insights from molecular phylogenetic analyses

Носов

Tikhomirov

Мачс

et al. 2019

Nordic Journal of Botany

View full text Add to dashboard Cite

In this study, sequence data from the inert nuclear region ITS1‐5.8S rDNA‐ITS2 and the chloroplast region trnL–F, as well as a few morphological characters, are analysed to the relationships among known annual Poa (bluegrasses). It is shown that all taxa from the Poa annua aggregate distinguished by lemma characters and growth form have identical ITS and trnL–trnF sequences, all ITS sequences of this aggregate are the same as thethose of P. supina, and all trnL–trnF sequences are homologous with those of P. infirma. Furthermore, no differences were found between unusual morphotypes of P. supina with short spinules on their panicle branches and typical plants, but Siberian samples were found to have a slightly differentiated trnL–trnF region. These results suggest a hybrid origin of the Asian annual bluegrasses. Their maternal genome is close to that of P. sect. Homalopoa, but their ITS sequences are different. Some ITS sequences from annual Asian bluegrasses are resolved among representatives of P. sect. Stenopoa while for other (morphologically closely similar) species they fall in a clade with representatives of P. sect. Malacanthae. The latter group is distant from P. sect. Ochlopoa and is better treated as a separate section, viz P. sect. Acroleucae. The American annual bluegrasses are heterogeneous and also rather distant from P. sect. Ochlopoa. Poa chapmaniana, a species with cleistogamic flowers, is nested among the basal Subantarctic sections, far away from the taxa with which it has previously been considered related. It is indeed closer to P. sect. Ochlopoa than to other annual American bluegrasses. Thus, the studied annual species in fact belong to four independent evolutionary lines (or six including the separate genus Eremopoa and the Turkish Poa jubata), one of which, Acroleucae, has gone through three reticulation events. As in previous studies, our analysis did not support the generic status of P. sect. Ochlopoa.

show abstract

Section: Discussionsupporting

confidence: 92%

“…Chloroplast sequence data shows that the maternal genome of the P. annua agg. was inherited from the diploid Mediterranean P. infirma (Tutin , 1957, Chrtek and Jirásek 1962, Darmency and Gasquez , Soreng et al , Mao and Huff ) (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

On polyphyly of the former section Ochlopoa and the hybridogenic section Acroleucae (Poa , Poaceae): insights from molecular phylogenetic analyses

Носов

Tikhomirov

Мачс

et al. 2019

Nordic Journal of Botany

View full text Add to dashboard Cite

show abstract

“…Poa annua has been proposed to be an allotetraploid, hybrid species derived from the diploid species P. supina and Poa infirma Kunth. (Tutin, 1952; Darmency & Gasquez, 1997). The nucleic acid sequences, and consequently the protein sequences, obtained using primers ACcp4 and ACcp2R were identical in all three species (Fig.…”

Section: Discussionmentioning

confidence: 99%

‘Universal’ primers for PCR‐sequencing of grass chloroplastic acetyl‐CoA carboxylase domains involved in resistance to herbicides

Délye

Michel

2005

Weed Research

View full text Add to dashboard Cite

Summary Primers were designed to amplify two regions involved in sensitivity to herbicides inhibiting the plastidic acetyl‐CoA carboxylase (ACCase) from grasses (Poaceae). The first primer pair amplified a 551‐bp amplicon containing a variable Ile/Leu codon at position 1781 in Alopecurus myosuroides sequence. The second primer pair amplified a 406‐bp amplicon containing four variable codons (Trp/Cys, Ile/Asn, Asp/Gly, Gly/Ala) at positions 2027, 2041, 2078 and 2096, respectively, in A. myosuroides sequence. Both primer pairs amplified the targeted fragments from genes encoding plastidic ACCases, but not from the very similar genes encoding cytosolic ACCases. Clear DNA sequences were obtained from fresh or dried plant material from the field, and from 29 various grass species. Sequences revealed that the gene encoding plastidic ACCase in Poa annua and Festuca rubra contained a Leu1781 codon, in agreement with both species being inherently tolerant to herbicides inhibiting ACCase. Sequencing confirmed the hybrid origin of P. annua. Compared with ACCase enzyme assay, polymerase chain reaction is faster, can be performed from a single plant and suppresses the need for radioactive experiments. It can be completed with basic molecular biology laboratory equipment. It is the tool of choice for diagnosing resistance caused by alteration(s) of the plastidic ACCase.

show abstract

“…annua and P. supina both display an excellent tolerance to low mowing heights which would improve the utility of P. arachnifera [22,23]. Some variants of P. annua can be extremely similar to P. supina in their morphological features, life history traits, and flowering requirements [8]. The KO-based CISP primers (KO-F/KO-R), however, were able to produce an identifying banding pattern for the three species (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

“…and supine bluegrass (Poa supina Schrad.) [8]. Randomly amplified polymorphic DNA (RAPD) is a cheap, less laborious marker system that has been used to characterize different cultivars of Kentucky bluegrass [9].…”

Section: Introductionmentioning

confidence: 99%

Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass)

2018

View full text Add to dashboard Cite

Abstract:Interspecific hybridization has been attempted to combine the heat and drought of Poa arachnifera Torr. with the turf quality characteristics of several Poa species. Confirmation of an F 1 hybrid through morphological analysis of vegetative and flowering characteristics is often time consuming and ambiguous. Ent-kaurene oxidase (KO) has been sequenced in rice, barley, and wheat. In rice, each of the five copies of KO gene has unique lengths for the first intron. Conserved intron spanning primers (CISP) can be used as a DNA marker to exploit variations of intron lengths that flank conserved gene sequences. In the present study, we developed CISP to sequence partial genomic fragments of the KO gene from seven Poa species. Through sequence analysis, species-specific primers were also developed to produce co-dominant markers that can be used to identify interspecific hybrids between Texas bluegrass and six other Poa species used in the present study.

show abstract

Spontaneous hybridization of the putative ancestors of the allotetraploid Poa annua

Cited by 21 publications

References 22 publications

On polyphyly of the former section Ochlopoa and the hybridogenic section Acroleucae (Poa , Poaceae): insights from molecular phylogenetic analyses

On polyphyly of the former section Ochlopoa and the hybridogenic section Acroleucae (Poa , Poaceae): insights from molecular phylogenetic analyses

‘Universal’ primers for PCR‐sequencing of grass chloroplastic acetyl‐CoA carboxylase domains involved in resistance to herbicides

Development of ent-kaurene Oxidase-Based Conserved Intron Spanning Primers for Species Identification in the Genus Poa (Poaceae; Bluegrass)

Contact Info

Product

Resources

About