Spontaneous Cytokine Production in Children According to Biological Characteristics and Environmental Exposures

Figueiredo, Camila A.; Alcântara-Neves, Neuza Maria; Veiga, Rafael Valente; Amorim, Leila Denise Alves Ferreira; Dattoli, Vitor Camilo Cavalcante; Mendonça, Lívia Ribeiro; Junqueira, Samuel B; Genser, Bernd; Santos, Mariese Conceição Alves dos; Carvalho, Lain Carlos Pontes de; Cooper, Philip J.; Rodrigues, Laura C.; Barreto, Maurício L.

doi:10.1289/ehp.0800366

Cited by 34 publications

(50 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As well, future investigations should strive to analyze research participants’ socio-economic and environmental data as there is evidence that a number of environmental exposures [33] including the presence of a household latrine [52] have a modulatory effect on serum IL-10 levels. Furthermore, emerging evidence suggests that there is significant genetic component to susceptibility or resistance to STH infection [52], and that there may IL-10 genetic polymorphisms that protect against helminthic infections [54].…”

Section: Discussionmentioning

confidence: 99%

Interleukin-10 and soil-transmitted helminth infections in Honduran children

2015

View full text Add to dashboard Cite

BackgroundSoil-transmitted helminths (STH) establish chronic infections in the human intestine. The host reacts to these infections with a dominant T-helper type 2 cell (Th2) response that while attempting to control the worm population, can also provide an anti-inflammatory environment favourable for parasite survival. Regulatory cytokine interleukin 10 (IL-10) has been proposed as a key molecule involved in the attenuation of chronic inflammation and the ensuing tolerance for these helminth parasites. The objective of this study was to determine whether STH-infected children from an endemic community had increased circulating IL-10 levels when compared to non-infected children.ResultsA total of 39 children (25 boys and 14 girls, 7–15 years of age) were enrolled in study. Utilizing the Kato-Katz method to detect intestinal helminthiases, 10 children were non-infected and 29 were harbouring STH infections by Ascaris lumbricoides, Trichuris trichiura and/or hookworms. Of the 29 infected children, 11 had single-species infections and 18 were polyparasitized with two or three STH species. Serum samples from all 39 children were tested for IL-10 serum concentrations, out of which 12 had undetectable levels while 27 had levels ranging from 0.4-105 pg/mL. Excluding extreme outlying values, 25 samples had IL-10 concentration values ranging from 0.4 -7.2 pg/mL. Differences in IL-10 levels among non-parasitized, monoparasitized, and polyparasitized groups were not statistically significant. However, children infected with any of the three STH species investigated had higher IL-10 levels than non-parasitized children (geometric means: 0.89 pg/mL vs. 0.74 pg/mL, p = 0.428). Similarly, polyparasitized children had higher IL-10 levels than both monoparasitized and non-parasitized children (1.04 pg/mL, 0.69 pg/mL, and 0.74 pg/mL, respectively, p = 0.481). A significant moderate negative correlation between IL-10 levels and children’s age was found, but no correlations were observed between IL-10 levels and intensity of infection by any of the parasite species investigated.ConclusionsWe found no strong evidence for an association between STH infection and serum IL-10 concentration levels. However, the trends identified here warrant further investigation. Additional research is needed to expand the current understanding of the immune response elicited by STH infections in children living in endemic communities.

show abstract

Section: Discussionmentioning

confidence: 99%

Interleukin-10 and soil-transmitted helminth infections in Honduran children

2015

View full text Add to dashboard Cite

show abstract

“…Just as direct comparisons would provide a better understanding of why there is variability between observations of innate immune development in resource rich settings 31-33 versus resource poor settings 34-37 . For example, lacking access to running water correlates positively with expression of IL-10 in childhood 38 . The majority (58%) of HEU infants in our study lacked access to running water, whereas only 15% of UE infants did.…”

Section: Discussionmentioning

confidence: 99%

Altered Innate Immune Development in HIV-Exposed Uninfected Infants

Reikie¹,

Adams²,

Leligdowicz³

et al. 2014

JAIDS Journal of Acquired Immune Deficiency Syndromes

View full text Add to dashboard Cite

BACKGROUND Early in life HIV-exposed uninfected (HEU) infants are at an increased risk of morbidity and mortality from infectious disease compared to HIV-unexposed (UE) infants. To improve our understanding of the mechanisms underlying their increased risk, we contrasted innate immune development between HEU and UE infants in a developing world setting, where early-life infectious disease risk is exceptionally high. METHODS A prospective longitudinal cohort of HEU and UE newborns was established and the most detailed characterization to date of HEU infant immune development was performed. Single-cell cytokine production was analyzed by flow cytometry after stimulation of whole blood with pathogen associated molecular patterns (PAMP). RESULTS Monocyte, classical dendritic cell and plasmacytoid dendritic cell composition was similar between HEU and UE infants throughout the first year of life. However, HEU mononuclear cells mounted an enhanced pro-inflammatory response to PAMP stimulation, both in quantity of cytokine produced per-cell and in proportion of responder cells. Significant differences in cytokine production were detected on the single cell level in a PAMP-specific pattern, but only at 2 and 6 weeks of age; all differences normalized by 12 months of age. CONCLUSIONS This time course of innate immune deviation early in life corresponds to the clinical window of vulnerability to infections in HEU infants and may be at least partially responsible for their increased morbidity and mortality from infectious disease.

show abstract

“…The prevalence of wheezing in this city in the past 12 months in school children aged 12 to 13 years was reported to be very high (27.1%) (29). The design of this study has been reported elsewhere (6,13,29). In short, the study population included 1,445 children recruited in infancy for a prospective study measuring the impact of a citywide sanitation program on childhood morbidity (5).…”

Section: Methodsmentioning

confidence: 99%

“…We previously demonstrated that children living in circumstances of poor hygiene without access to sanitation or clean water during the first 3 years of life have elevated spontaneous production of IL-10 up to 8 years later in life (13).…”

mentioning

confidence: 99%