SPME – A valuable tool for investigation of flower scent

Barták, Petr; Bednář, Petr; Čáp, Lubomír; Ondráková, Lenka; Stránský, Zdeněk

doi:10.1002/jssc.200301381

Cited by 47 publications

(28 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The best results were obtained for fibres with mixed phases, a liquid phase (polidimethylsiloxane) and a porous solid (carboxen, divinylbenzene or both), such as DVB-CAR-PDMS or CAR-PDMS fibres. Barták et al 27 proposed a HS-SPME method for in situ analysis of floral scent. Rosemary was used to optimize the most important variables of SPME.…”

Section: Introductionmentioning

confidence: 99%

Determination of volatile compounds in antioxidant rosemary extracts by multiple headspace solid-phase microextraction and gas chromatography

Carrillo

Tena

2006

Flavour Fragr. J.

View full text Add to dashboard Cite

Rosemary extracts are commercialized as natural antioxidants that delay the lipid oxidation process. Residual amounts of rosemary odour-responsible volatile compounds, such as borneol, camphor and verbenone, can remain in the final extract. The presence of these compounds may be undesirable when the extracts are used as food or cosmetic additives. In this paper a MHS-SPME method is proposed to quantify 10 rosemary odour-responsible compounds in a commercial solid rosemary extract. Multiple headspace solid-phase microextraction (MHS-SPME) is a recent technique proposed to eliminate the matrix effect on the quantitative analysis of solid samples. This technique performs few consecutive extractions from the same sample, and the total area corresponding to an exhaustive extraction is calculated as the sum of individual area values obtained from each extraction or by using an equation. The most influential variables of the extraction process, such as type of fibre, temperature and extraction time, were studied. Extraction was carried out using 2.5 mg solid extract and a divinylbenzene-carboxen-polydimethylsiloxane (DVB-CAR-PDMS) fibre at 55°C for 60 min; calibration was carried out using aqueous standard solutions containing 1% (v/v) ethanol at 40°C for 45 min. The features of the method were established using aqueous standard solutions. Precision was 4-15%, expressed as RSD, and the method allowed detection limits of 0.4 -47 ng for p-cymene and verbenone, respectively. Finally, the method was applied to analyse a rosemary solid extract, and verbenone (356 µ µ µ µ µg/g), borneol (190 µ µ µ µ µg/g) and camphor (136 µ µ µ µ µg/g) were found to be the most abundant compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of volatile compounds in antioxidant rosemary extracts by multiple headspace solid-phase microextraction and gas chromatography

Carrillo

Tena

2006

Flavour Fragr. J.

View full text Add to dashboard Cite

show abstract

“…It is a fast, easy, convenient and effective technique with good future prospects. 2,[9][10][11][12][13][14][15][16][17][18] The genus Artemisia (family Asteraceae) is represented in Turkey by 24 species. 19,20 Several Artemisia spp.…”

Section: Introductionmentioning

confidence: 99%

Headspace-SPME and hydrodistillation of two fragrantArtemisia sp.

2005

View full text Add to dashboard Cite

The family Asteraceae comprises aromatic plants with a strong distinctive fragrance. Artemisia species belonging to this family are known to contain essential oils. Fresh aerial parts of Artemisia spicigera C. Koch and A. scoparia Waldst. et Kit. were subjected to headspace-SPME (HS-SPME) and the same plants were hydrodistilled (HD) after drying to obtain the profiles of volatiles. Headspace volatiles were analysed by GC-MS, whereas the essential oils were analysed by both GC and GC-MS. Camphor (37.5% and 43.9%) was found as the main constituent in both oils and in SPME samples of A. spicigera. However, in the oil sample of A. scoparia, capillene (1-phenylhexa-2,4-diyne) (53.1%) was found as the main constituent, while β β β β β-pinene (20.8%), β β β β β-caryophyllene (16.4%), (Z)-β β β β β-ocimene (16.4%), myrcene (12.8%), and limonene (11.0 %) were the main components in the headspace-SPME, respectively.

show abstract

“…Recently, a novel sampling technique, solid-phase microextraction (SPME) introduced by Pawliszyn et al has been developed for analysis of floral fragrances. [9][10][11][12][13][14][15][16][17][18] Headspace SPME with GC-MS has been demonstrated to be a rapid, simple, sensitive and solvent-free method for analysis of volatiles in plant emissions. In our previous studies, headspace SPME was developed for the determination of methyl salicylate released from tomato plants infected by tobacco mosaic virus and volatile compounds from flowers.…”

Section: Introductionmentioning

confidence: 99%

Application of HS‐SPME and GC‐MS to Characterization of Volatile Compounds Emitted from Osmanthus Flowers

Deng

Song

2004

Annali di Chimica

View full text Add to dashboard Cite

Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was developed for characterization of volatile compounds emitted from two varieties Osmanthus flowers of O. fragrans var. latifolius and O. fragrans var. thunbergii. The SPME parameters were studied, the optimum conditions of a 65 microm carbowax/divinylbenzene (CW/DVB), extraction temperature of 22 degrees C and extraction time of 10 min were obtained and applied to extraction of the volatile emissions. Fourteen compounds released from both varieties of Osmanthus flowers were separated and identified by GC-MS, which mainly included alpha-linalool, beta-linalool, trans-linalool oxide, cis-linalool oxide, alpha-lonone, beta-lonone, capraldehyde and decalactone. By comparing their peak areas, we found that the sums of the fourteen compounds from the two Osmanthus flowers were very close, while the relative contents of individual volatile compounds in the two emissions were very different. The relative content of alpha-linalool and beta-linalool in O. fragrans var. latifolius were 39.46% and 0.51%, while in O. fragrans var. thunbergii were 9.53% and 27.71%. Due to their different relative contents, the two varieties of flower have different fragrances.

show abstract

SPME – A valuable tool for investigation of flower scent

Cited by 47 publications

References 42 publications

Determination of volatile compounds in antioxidant rosemary extracts by multiple headspace solid-phase microextraction and gas chromatography

Determination of volatile compounds in antioxidant rosemary extracts by multiple headspace solid-phase microextraction and gas chromatography

Headspace-SPME and hydrodistillation of two fragrantArtemisia sp.

Application of HS‐SPME and GC‐MS to Characterization of Volatile Compounds Emitted from Osmanthus Flowers

Contact Info

Product

Resources

About