Application of HS‐SPME and GC‐MS to Characterization of Volatile Compounds Emitted from Osmanthus Flowers

Abstract

Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was developed for characterization of volatile compounds emitted from two varieties Osmanthus flowers of O. fragrans var. latifolius and O. fragrans var. thunbergii. The SPME parameters were studied, the optimum conditions of a 65 microm carbowax/divinylbenzene (CW/DVB), extraction temperature of 22 degrees C and extraction time of 10 min were obtained and applied to extraction of the volatile emissions. Fourteen compounds released from both varieties of Osmanthus flowers were separated and identified by GC-MS, which mainly included alpha-linalool, beta-linalool, trans-linalool oxide, cis-linalool oxide, alpha-lonone, beta-lonone, capraldehyde and decalactone. By comparing their peak areas, we found that the sums of the fourteen compounds from the two Osmanthus flowers were very close, while the relative contents of individual volatile compounds in the two emissions were very different. The relative content of alpha-linalool and beta-linalool in O. fragrans var. latifolius were 39.46% and 0.51%, while in O. fragrans var. thunbergii were 9.53% and 27.71%. Due to their different relative contents, the two varieties of flower have different fragrances.

Neuroprotection and free radical scavenging effects of Osmanthus fragrans

“…O. fragrans is used in cosmetics for the hair and skin, but is mostly used for aromatic therapy. In addition, O. fragrans in medicinal uses with an anti-tussive of its flowers and its essential oil used as a flavouring [1].…”

Characterization of volatile compounds in flowers from four groups of sweet osmanthus (Osmanthus fragrans) cultivars

“…The last cultivar group blooms almost every month, and fully blooms in spring and autumn with white/light yellow flowers (four-season osmanthus). Sweet osmanthus flowers contain abundant volatiles, with 14Á66 volatiles reported, the variability likely depending on experimental methods and cultivar (Deng et al 2004;Li et al 2008;Tian et al 2008;Wang et al 2009a;Yang et al 2005Yang et al , 2010. Fourteen volatiles were detected in live flowers of two sweet osmanthus cultivars, one belonging to the Thunbergii Group and the other to the Latifolius Group.…”

“…Fourteen volatiles were detected in live flowers of two sweet osmanthus cultivars, one belonging to the Thunbergii Group and the other to the Latifolius Group. The two cultivars had different relative proportions of volatile compounds, resulting in different fragrances (Deng et al 2004). Sixty-six volatiles were detected in shade-dried flowers in of 'Wanyingui' and 'Yaotiaoshunv' (Latifolius Group) and 'Guifeihong' (Aurantiacus Group) (Yang et al 2010).…”

cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

“…• C for 3 min and analysed by gas chromatography-mass spectrometry (GC-MS) (Deng et al 2004). Relative content of ionone refered to the proportion of ionone in total volatile oil per flower.…”