Splenic Replenishment of Synergistic Ability to Bone Marrow and Thymic Cells of Neonatally Splenectomized Cba Mice

Bucsi, Rose A.; Borek, F.; Battisto, Jack R.

doi:10.1084/jem.136.4.761

Cited by 14 publications

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References 20 publications

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“…Over the past several years, however, a number of unique spontaneous animal models have been discovered that lend themselves to versatile application in the study of host immunity to infectious agents. These include the congenitally athymic (nude) and hereditarily asplenic (Dh/+) mice (8,12).…”

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“…Another animal model that has direct relevance to these kinds of studies is the hereditarily asplenic mouse. In 1959 the hereditary absence of the spleen was discovered as a mutant in a mouse colony in England (8). Asplenia is inherited as an autosomal dominant disorder with 100% penetrance.…”

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“…Heterozygotes, besides lacking spleens, have preaxial triphalangy and/or poly-, syn-, or oligodoctyly of the hind feet; affected mice are thus referred to as carrying the Dh gene for dominant hemimelia (i.e., Dh/+). Because of the effect of the spleen on the maturation of T-cell function, Dh/+ mice, in contrast to splenic littermate controls (+/+), manifest a major age-dependent qualitative deficiency of T-cell function as well as a quantitative reduction in serum immunoglobulins (8).…”

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Infectious Agents in Immunodeficient Murine Models: Pathogenicity of Nocardia asteroides in Congenitally Athymic (Nude) and Hereditarily Asplenic (Dh/+) Mice

1978

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Congenitally athymic (Nu/Nu), hereditarily asplenic (Dh/+), and littermate control mice were given intravenous injections of homogeneous cell suspensions of the virulent Nocardia asteroides GUH-2. Kill curve, 50% lethal dose, and kidney clearance data were obtained over a period of 3 months postinfection. N. asteroides initiated both an acute infectious process and a chronic, progressive disease in these animals when given intravenously. The heterozygous (Nu/+) mice appeared to be slightly more susceptible to the acute phase of infection than their nude littermates. In contrast, nude mice were at least 50 times more susceptible to chronic nocardial infection than were the heterozygous (Nu/+) controls. Swiss Webster specific pathogen-free mice were similar to heterozygous (Nu/+) mice in their susceptibility to N. asteroides. The hereditarily asplenic (Dh/+) mice were not as susceptible to lethal infection as were nude mice. However, asplenic mice demonstrated an inability to eliminate nocardia from infected kidneys, whereas their littermate control (+/+) mice were able to mount an effective response and destroy most of the organisms within the kidneys. Similar observations were noted when nude and heterozygous (Nu/+) littermate mice were infected in the footpad. The nude mice developed a systemic infection and died within 4 weeks with little inflammation of the footpad and no macroscopic lesions. In contrast, heterozygous (Nu/+) mice developed extensive local abscesses in the foot that persisted for at least 4 weeks. There was no animal death and no evidence of dissemination. The data presented herein indicate that T cells are essential for adequate host response against infection with a virulent strain of N. asteroides.

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Infectious Agents in Immunodeficient Murine Models: Pathogenicity of Nocardia asteroides in Congenitally Athymic (Nude) and Hereditarily Asplenic (Dh/+) Mice

1978

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Bursal and postbursal cells in chicken. Occurrence of postbursal cells in bone marrow, thymus and spleen

Toivanen

Tamminen

1974

Eur J Immunol

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Functional development of the interacting cells in the immune response

Russell

Golub

1977

Eur J Immunol

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Neonatal splenectomy and congenital absence of the spleen were shown to be associated with lack of primary IgM antibody synthesis, deficient bone marrow-thymus cell synergism. altered mitogen responsiveness and depressed homing patterns of thymocytes to the spleen. Using congenitally asplenic animals, altered B-T cell cooperation was manifest at the T cell but not B cell level. This correlated with the ability of thymus cells from either congenitally asplenic or neonatally splenectomized (NSx) animals to respond to the B cell mitogen lipopolysaccharide although there were no detectable Ig=ells present. To determine the role played by the spleen in the development of T cell function, NSx animals were given either irradiated or normal spleen grafts. Both irradiated and unirradiated grafts restored normal B-T cooperation and normal mitogenic responses of thymus cells. However, neither type of spleen graft was successful in restoring normal homing patterns to the spleen. It is concluded that the splenic microenvironment influences T cell maturation very early in life and that only some of the effects attributable to the absence of the spleen can be reversed by the reintroduction of this tissue as a graft.

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Splenic Replenishment of Synergistic Ability to Bone Marrow and Thymic Cells of Neonatally Splenectomized Cba Mice

Cited by 14 publications

References 20 publications

Infectious Agents in Immunodeficient Murine Models: Pathogenicity of Nocardia asteroides in Congenitally Athymic (Nude) and Hereditarily Asplenic (Dh/+) Mice

Infectious Agents in Immunodeficient Murine Models: Pathogenicity of Nocardia asteroides in Congenitally Athymic (Nude) and Hereditarily Asplenic (Dh/+) Mice

Bursal and postbursal cells in chicken. Occurrence of postbursal cells in bone marrow, thymus and spleen

Functional development of the interacting cells in the immune response

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