Splash dispersal of conidia of <i>Mycocentrospora acerina</i> in the field

Evenhuis, A.; Verdam, B.; Zadoks, J.C.

doi:10.1046/j.1365-3059.1997.d01-42.x

Cited by 21 publications

(17 citation statements)

References 16 publications

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“…Sampling procedures may involve passive collection of spores by gravitational deposition (14) and/or sampling specific volumes of air with "active" spore-trapping devices (1,9,18). Such techniques require considerable amounts of time and expertise if accurate counts are to be obtained.…”

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confidence: 99%

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Kennedy¹,

Wakeham²,

Byrne³

et al. 2000

Appl Environ Microbiol

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We describe a new microtiter immunospore trapping device (MTIST device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. This device can be used for rapid detection and immunoquantification of ascospores of Mycosphaerella brassicicola and conidia of Botrytis cinerea by an enzymelinked immunosorbent assay (ELISA) under controlled environmental conditions. For ascospores of M. brassicicola correlation coefficients (r 2 ) of 0.943 and 0.9514 were observed for the number of MTIST device-impacted ascospores per microtiter well and the absorbance values determined by ELISA, respectively. These values were not affected when a mixed fungal spore population was used. There was a relationship between the number of MTIST device-trapped ascospores of M. brassicicola per liter of air sampled and the amount of disease expressed on exposed plants of Brassica oleracea (Brussels sprouts). Similarly, when the MTIST device was used to trap conidia of B. cinerea, a correlation coefficient of 0.8797 was obtained for the absorbance values generated by the ELISA and the observed number of conidia per microtiter well. The relative collection efficiency of the MTIST device in controlled plant growth chambers with limited airflow was 1.7 times greater than the relative collection efficiency of a Burkard 7-day volumetric spore trap for collection of M. brassicicola ascospores. The MTIST device can be used to rapidly differentiate, determine, and accurately quantify target organisms in a microflora. The MTIST device is a portable, robust, inexpensive system that can be used to perform multiple tests in a single sampling period, and it should be useful for monitoring airborne particulates and microorganisms in a range of environments.

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confidence: 99%

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Kennedy¹,

Wakeham²,

Byrne³

et al. 2000

Appl Environ Microbiol

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“…These observations may indicate that relatively high wind speed is essential for air transport of M. acerina conidia. Evenhuis et al (1997) observed that wind speed had only small effects on spore catches. They concluded that the wind does not play a role in the liberation of conidia, but may contribute to the impaction of spore-carrying droplets on spore traps.…”

Section: Discussionmentioning

confidence: 97%

“…If splash dispersal of the conidia from soil to the leaves is the most important way of conidial spread (Rintelen & Klewitz, 1976;Wall & Lewis, 1980a,b), this may explain the limited records of conidia in the present experiment. Evenhuis et al (1997) found that sporulation of M. acerina in caraway was stimulated by rainfall (] 2 mm) and temperatures around 15°C. Solar radiation had a negative effect on sporulation.…”

Section: Discussionmentioning

confidence: 99%

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Records of AirborneMycocentrospora acerinaConidia in and Near Carrot Plots

Hermansen¹,

Amundsen²

1999

Acta Agriculturae Scandinavica, Section B - Soil & Plant Sc

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The occurrence of Mycocentrospora acerina conidia in the air, in or close to carrot plots, was examined by the use of volumetric spore traps at three locations, in connection with crop-rotation experiments in Norway. The traps were operated during the late summers of 1985-1987 and in 1995. At two locations, M. acerina conidia were recorded on a limited number of days. Only a few conidia were trapped outside the plot. At one of the locations, no conidia were observed. The highest conidial record, 47 conida per m 3 air, was observed inside the carrot plot, during 1 h in September 1985 at one location. Only one or two conida were trapped on most of the other days. The conidial records in relation to climatic parameters are discussed. Aerial spread of M. acerina conidia appears to be insignificant in the climatic conditions of Norway.

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“…M. acerina that establishes in the rhizosphere or in the crown of the root is probably the main source of inoculum of this pathogen. Splash dispersal of conidia of M. acerina is possible within fields, but aerial spread by wind of these conidia appears to be insignificant (Evenhuis et al 1997;. M. acerina is a polyphageous fungus including several weeds as hosts (Hermansen 1992).…”

Section: Discussionmentioning

confidence: 99%

Detection and prediction of post harvest carrot diseases

et al. 2011

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Specific PCR primers were developed for identifying two post harvest pathogens, Mycocentrospora acerina and Fibularhizoctonia carotae, which cause liquorice rot and crater rot respectively, during prolonged low temperature storage of carrots. The methods allow routine detection of less than 0.3 pg of M. acerina DNA and less than 0.03 pg F. carotae DNA, even in the presence of large excess of plant or soil DNA. Standard PCR and quantitative PCR gave similar results and either method could be used in a practical situation. Experiments were carried out testing these methods on different types of carrot tissue-and soil-samples. Soil was sampled before sowing, and soil adhering to the roots or root tissue was sampled at different times during the growing season or at harvest. Soil adhering to the carrots at harvest had the best predictive ability for liquorice rot development during storage (R 2 predicted 74.9% using standard PCR), but samples taken during the growing season also gave reasonably good predictive ability values. PCR data from soil samples taken in the spring were not as good as a predictor for this disease. A dense sampling strategy using 20 m between sampling points generally gave better correlation between PCR data and disease data than using 40 m between the sampling points. Use of the developed methods in an IPM strategy for liquorice rot is discussed. For crater rot the correlation between PCR data and disease data was generally poor for all types of samples. These results are discussed in relation to the biology of F. carotae.

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Splash dispersal of conidia of Mycocentrospora acerina in the field

Cited by 21 publications

References 16 publications

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Records of AirborneMycocentrospora acerinaConidia in and Near Carrot Plots

Detection and prediction of post harvest carrot diseases

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