Spirulina Platensis Protects Against Renal Injury in Rats with Gentamicin-Induced Acute Tubular Necrosis

Abstract

The present study was carried out to evaluate the renoprotective antioxidant effect of Spirulina platensis on gentamicin-induced acute tubular necrosis in rats. Albino-Wistar rats, (9male and 9 female), weighing approximately 250 g, were used for this study. Rats were randomly assigned to three equal groups. Control group received 0,9 % sodium chloride intraperitoneally for 7 days at the same volume as gentamicin group. Gentamicin group was treated intraperitoneally with gentamicin, 80 mg/kg daily for 7 days. Gentamicin+spirulina group received Spirulina platensis 1000 mg/kg orally 2 days before and 7 days concurrently with gentamicin (80 mg/kg i.p.). Nephrotoxicity was assessed by measuring plasma nitrite concentration, stabile metabolic product of nitric oxide with oxygen. Plasma nitrite concentration was determined by colorimetric method using Griess reaction. For histological analysis kidney specimens were stained with hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) stain. Plasma nitrite concentration and the level of kidney damage were significantly higher in gentamicin group in comparison both to the control and gentamicin+spirulina group. Spirulina platensis significantly lowered the plasma nitrite level and attenuated histomorphological changes related to renal injury caused by gentamicin. Thus, the results from present study suggest that Spirulina platensis has renoprotective potential in gentamicin-induced acute tubular necrosis possibly due to its antioxidant properties.

Experimental Gentamicin Nephrotoxicity and Agents that Modify it: A Mini-Review of Recent Research

“…Similar findings were reported by Avdagic et al. [33] using similar doses of S. platensis and GM, except that the treatment was carried out for 9 days. The three papers suggested that the basis of the nephroprotective action of Spirulina was its antioxidant activity against the generation of free radicals induced by GM.…”

Protective Role of Spirulina platensis against Acute Deltamethrin-Induced Toxicity in Rats

“…Many previous literatures showed the hepatoprotective effects of SP and its active constituents against drugs, chemicals and xenobiotics [24], [55]–[56]. The nephroprotective effects of SP have been reported against renal injury induced by gentamicin [27], [57] as well as oxalate [58]–[59]. Moreover, the antioxidant effects of SP have been reported against sodium fluoride-induced oxidative alterations in offspring of pregnant rats [60].…”

Natural remedies for non‐steroidal anti‐inflammatory drug‐induced toxicity

“…S. platensis was proved to have normalized the histopathological changes caused by dibutyl nitrosamine-induced rats (Ismail, 2009). S. platensis was proved to have minimized the kidney injury caused in gentamicin-and nitrate-induced rats at the dosage 1000 mg kg À1 (Avdagić et al, 2008).…”

“…S. fusiformis was reported to treat renal toxicity against cisplatininduced toxicity in rats at the dosage 500 mg kg À1 (Avdagić et al, 2008). S. fusiformis was reported to protect against genotoxicity caused by cisplatin-and urethane-induced toxic mice at the dosage 250 mg kg À1 (Rasool et al, 2006).…”