Spirulina maxima derived marine pectin promotes the in vitro and in vivo regeneration and wound healing in zebrafish

Edirisinghe, Shan Lakmal; Rajapaksha, Dinusha C.; Nikapitiya, Chamilani; Oh, Chulhong; Lee, Kyoung-Ah; Kang, Do‐Hyung; Zoysa, Mahanama De

doi:10.1016/j.fsi.2020.10.008

Cited by 23 publications

(16 citation statements)

References 43 publications

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“…This difference is perhaps unsurprising when the characteristics of skin in fish and humans is considered along with their differing physiologies and the demands of their environments. Of the genes associated with extracellular matrix processes, the transcriptional response of chemokine, cxcl12a , involved in the inflammatory response [ 50 , 51 ] and fibroblast proliferation during wound healing [ 52 , 53 ], was significantly upregulated by hCRTAC1-A, dlCrtac1a and 1b in SJD.1 fibroblasts at 6 h but not 24 h after damage. Cxcl12a has previously been shown to regulate the migration of several different zebrafish cell types [ 54 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?

et al. 2021

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Fish skin has been gaining attention due to its efficacy as a human-wound-treatment product and to identify factors promoting its enhanced action. Skin fibroblasts have a central role in maintaining skin integrity and secrete extra cellular matrix (ECM) proteins, growth factors and cytokines to rapidly repair lesions and prevent further damage or infection. The effects on scratch repair of the ubiquitous but poorly characterized ECM protein, cartilage acidic protein 1 (CRTAC1), from piscine and human sources were compared using a zebrafish SJD.1 primary fibroblast cell line. A classic in vitro cell scratch assay, immunofluorescence, biosensor and gene expression analysis were used. Our results demonstrated that the duplicate sea bass Crtac1a and Crtac1b proteins and human CRTAC-1A all promoted SJD.1 primary fibroblast migration in a classic scratch assay and in an electric cell impedance sensing assay. The immunofluorescence analysis revealed that CRTAC1 enhanced cell migration was most likely caused by actin-driven cytoskeletal changes and the cellular transcriptional response was most affected in the early stage (6 h) of scratch repair. In summary, our results suggest that CRTAC1 may be an important factor in fish skin promoting damage repair.

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Section: Discussionmentioning

confidence: 99%

Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?

et al. 2021

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“…In the study by Rajapaksha et al (2020), Spirulina maxima derives pectin nanoparticles that are able to reduce ROS dramatically and increase immune modulatory activity in zebrafish wounds. In this case, the researchers observed that the wound-healing markers, specifically, TGF-β1, TIMP2b, MMP-9, TNF-α, IL-1, and chemokines including ccl34a.4 and ccl34b.4, were upregulated [43,74]. Resultantly, rapid wound healing, formation of the neoepidermis, and restored pigmentation were witnessed.…”

Section: Nanoparticlesmentioning

confidence: 98%

“…This is further supported by Caraguel et al 2016 [38] who witness similar results in their in vivo investigation. Upon identifying the migration of cells towards dermal region due to increased level of EGF production in the basal epidermal, they claimed that the result acquired by Lisse et al (2016) [36] [42], and Edirisinghe et al 2020 [43] studied the mechanism of full-thickness wound healing using zebrafish. Despite differences in the strain used, the results from 100% of the studies showed promising results.…”

Section: Zebrafish As a Model To Study The Mechanism Of Cutaneous Wound Healingmentioning

confidence: 99%

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Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

et al. 2021

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In humans, cutaneous wounds may heal without scars during embryogenesis. However, in the adult phase, the similar wound may undergo a few events such as homeostasis, blood clotting, inflammation, vascularization, and the formation of granulation tissue, which may leave a scar at the injury site. In consideration of this, research evolves daily to improve the healing mechanism in which the wound may heal without scarring. In regard to this, zebrafish (Danio rerio) serves as an ideal model to study the underlying signaling mechanism of wound healing. This is an important factor in determining a relevant drug formulation for wound healing. This review scrutinizes the biology of zebrafish and how this favors the cutaneous wound healing relevant to the in vivo evidence. This review aimed to provide the current insights on drug discovery for cutaneous wound healing based on the zebrafish model. The advantages and challenges in utilizing the zebrafish model for cutaneous wound healing are discussed in this review. This review is expected to provide an idea to formulate an appropriate drug for cutaneous wound healing relevant to the underlying signaling mechanism. Therefore, this narrative review recapitulates current evidence from in vivo studies on the cutaneous wound healing mechanism, which favours the discovery of new drugs. This article concludes with the need for zebrafish as an investigation model for biomedical research in the future to ensure that drug repositions are well suited for human skin.

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“…Microalgae Spirulina is a photosynthetic cyanobacterium that has been used to improve reproductive efficiency in in vivo bovine studies [ 33 ]. Recently, S. maxima -based pectin (SmP), and their modified two products namely, modified SmP (MSmP) and its nanoparticles (MSmPNPs) were investigated for different bioactivities in in vitro and in vivo studies [ 37 , 38 , 39 ]. Modification of SmP remarkably enhanced their functionality via physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

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Tanga

et al. 2021

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Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.

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Spirulina maxima derived marine pectin promotes the in vitro and in vivo regeneration and wound healing in zebrafish

Cited by 23 publications

References 43 publications

Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?

Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?

Zebrafish as a Model System to Study the Mechanism of Cutaneous Wound Healing and Drug Discovery: Advantages and Challenges

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

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