2005
DOI: 10.1364/opex.13.000689
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Spiral phase contrast imaging in microscopy

Abstract: We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(i phi) on the diffracted light field in its Fourier plane. In the … Show more

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Cited by 538 publications
(295 citation statements)
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“…We propose here a filter that is based on a spiral phase. A spiral phase filter with an azimuthal period of 2;r is a good edge enhancer as has been demonstrated in recent publications [9,10]. We show here that a spiral phase filter with azimuthal period n behaves as a comer enhancer The comer detection property of this filter can be more clearly understood by analyzing the symmetry of its impulse response.…”
Section: Corner Detectionsupporting
confidence: 73%
See 1 more Smart Citation
“…We propose here a filter that is based on a spiral phase. A spiral phase filter with an azimuthal period of 2;r is a good edge enhancer as has been demonstrated in recent publications [9,10]. We show here that a spiral phase filter with azimuthal period n behaves as a comer enhancer The comer detection property of this filter can be more clearly understood by analyzing the symmetry of its impulse response.…”
Section: Corner Detectionsupporting
confidence: 73%
“…Two recent works have addressed rotation invariance in optical edge detection employing a spiral phase filter [9,10]. In the present work, we propose a new optical comer detection method based on a spiral phase filter…”
Section: Introductionmentioning
confidence: 99%
“…These OVs are localizations in space were the amplitude of the wave is zero and its phase is undefined [1][2][3], while in the surroundings the phase takes a helicoidal form, usually represented with the term exp(imθ), where θ is the azimuthal angle and m is the topological charge representing the number of 2π discontinuities of the wavefront [4]. Due to these features, OVs have been widely used in metrological applications [5,6], phase shifting interferometry [7], stellar choronography [8], among others. To generate the OVs, computer generated holograms (CGHs) [9], spiral phase plates (SPPs) [10] and vortex-producing lenses (VPL) [11] are commonly used.…”
Section: Introductionmentioning
confidence: 99%
“…Especially at high -values, a significant increase in illumination flux is achievable with only a modest reduction in contrast. Such a trade-off may be applicable within low-light imaging applications in either classical (17) or quantum domains (18).…”
Section: Discussionmentioning
confidence: 99%
“…We also note that the study of spiral phase filters, as applied to light of finite spatial coherence, is closely related to both spiral phase microscopy (17,18) and optical vortex coronagraphs (19).…”
Section: Introductionmentioning
confidence: 85%