SPIONs/3D SiSBA-16 based Multifunctional Nanoformulation for target specific cisplatin release in colon and cervical cancer cell lines

Abstract

Multifunctional nanomaterials can be used for dual applications: drug delivery as well as in bioimaging. In current study, we investigated potential use of silica based supports; 3D cage type SiSBA-16 (S-16), monodispersed hydrophilic spherical silica (HYPS) and mesocellular foam (MSU-F) for cisplatin (Cp) delivery. To obtain magnetic resonance characteristics, 10 wt% iron oxide was loaded through enforced adsorption technique. For pH stimuli responsive release of Cp, 10 wt%SPIONs/S-16 was functionalized with 3-(Aminopropyl)triethoxysilane (A) and poly acrylic acid (PAA) termed as 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp. By TEM analysis, the average diameter of the SPIONs was found to range between 10–60 nm. VSM analysis showed saturation magnetization over S-16, HYPS and MSU-F were in the following order: 10 wt%SPIONs/HYPS (4.08 emug−1) > 10 wt%SPIONs /S-16 (2.39 emug−1) > 10 wt%SPIONs/MSU-F (0.23 emug−1). Cp release study using dialysis membrane in PBS solution over 10 wt%SPIONs/S-16 nanoformulations showed highest cumulative release (65%) than 10 wt%SPIONs/MSU-F-A-Cp (63%), 10 wt%SPIONs/HYPS-A-Cp (58%), and Cp-F127/S-16 (53%), respectively. 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp were evaluated for in vitro target anticancer efficiency in human cancer cell lines (colon cancer (HCT 116), cervical cancer (HeLa)) and normal cells (Human embryonic kidney cells (HEK293) using MTT and DAPI staining. 10 wt%SPIONs/S-16-A-Cp treated Hela and HCT116 cancerous cell lines showed significant control of cell growth, apoptotic activity and less cytotoxic effect as compared to Cp and 10 wt%SPIONs/S-16. Target specific Cp release in the cells shows that 10 wt%SPIONs/S-16-A-Cp can be easily upgraded for magnetic resonance imaging capability.

Hybrid System for Local Drug Delivery and Magnetic Hyperthermia Based on SPIONs Loaded with Doxorubicin and Epirubicin

“…Multiple studies show that multimodal therapy, including the application of SPIONs, gives a synergetic effect in killing cancer cells more efficiently than sole therapy, making spectacular improvement of the therapeutic effects, e.g., greatly extend the lives of patients, even with the most aggressive brain cancers like glioblastoma [ 34 , 35 ], cervical cancer [ 36 ] or application in other biomedical fields like to prevent memory dysfunctions or cargo delivery [ 37 , 38 ]. In general, SPIONs have tremendous potential in surface engineering, so they have been widely investigated as a leading strategy towards many diseases [ 39 ], especially there, where macromolecule delivery is needed [ 40 ] for the facile release of conjugates from their surface [ 41 ].…”

Cisplatin delivery, anticancer and antibacterial properties of Fe/SBA-16/ZIF-8 nanocomposite

“…14 However, the benecial cytotoxic effect of cisplatin can be effectively utilized using several nanocarriers. 15,16 In the present study, cisplatin release at the acidic pH conditions of tumors and the anticancer and antibacterial activity of ZIF-8 was studied using a Fe/SBA-16/ZIF-8 nanoformulation. 10 wt% superparamagnetic iron oxide nanoparticles (SPIONs) were impregnated with mesoporous SBA-16, and then loaded with ZIF-8 ($13 mg).…”

Two-dimensional-Ti3C2 magnetic nanocomposite for targeted cancer chemotherapy

“…As part of the cisplatin adsorption study, the following nanocarriers (Ti 3 C 2 -Fe 3 O 4 @SiO 2 -FA and Ti 3 C 2 @ FA) (1 mL) loaded formulations were mixed separately with various masses of Cisplatin (0.5, 1, 1.5, 2, 3 mg) of Cisplatin in 20 mL of Phosphate-buffered saline solution (PBS) under an ice-cooled dark environment ( Jermy et al, 2019 ; 2021 ). After overnight stirring and sonication at room temperature, the solution mixture was centrifuged at 8,000 rpm for 10 min.…”