Spin labelling of human erythrocytes with nitroxide radicals

Chagalj, C.; DePaoli, T. C. P.; Hager, A; Palaoro, Luis Alberto; Celis, Emilio Rubín de; Farach, Horácio A.; Poole, Charles P.

doi:10.1007/bf02453343

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…The covalent bindings between these spin labels and the internal RBC -SH group prevents their return to the plasma and changes the RBC into label cells. [28][29] Nitroxide radical ESR signal was recorded at 20 8C using an X-band ESR Spectrometer Bruker ECS 106 with an ESR aqueous flat cell. The spectrometer settings for the nitroxide radicals were: field center 3440 G, sweep width 80 G, microwave frequency 9.7 GHz, microwave power 10 mW, time constant 327.68 ms, modulation frequency 50 KHz, modulation amplitude 0.205 G, and resolution 1024 points.…”

Section: Methodsmentioning

confidence: 99%

Circulating Blood Volume Determination Using Electronic Spin Resonance Spectroscopy

et al. 2006

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There have been numerous methods proposed to measure the circulating blood volume (CBV). Nevertheless, none of them have been massively and routinely accepted in clinical diagnosis. This study describes a simple and rapid method, on a rabbit model, using the dilution of autologous red cells labeled with a nitroxide radical (Iodoacetamide-TEMPO), which can be detected by electronic spin resonance (ESR) spectroscopy. Blood samples were withdrawn and re-injected using the ears' marginal veins. The average CBV measured by the new method/body weight (CBV(IAT)/BW) was 59 +/- 7 mL/kg (n = 33). Simultaneously, blood volume determinations using the nitroxide radical and (51)Cr (CBV(Cr)) were performed. In the plot of the difference between the methods (CBV(IAT) - CBV(Cr)) against the average (CBV(IAT) + CBV(Cr))/2, the mean of the bias was -1.1 +/- 6.9 mL and the limits of agreement (mean difference +/-2 SD) were -14.9 and 12.7 mL. Lin's concordance correlation coefficient p(c) = 0.988. Thus, both methods are in close agreement. The development of a new method that allows a correct estimation of the CBV without using radioactivity, avoiding blood manipulation, and decreasing the possibility of blood contamination with similar accuracy and precision of that of the "gold standard method" is an innovative proposal.

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Section: Methodsmentioning

confidence: 99%

Circulating Blood Volume Determination Using Electronic Spin Resonance Spectroscopy

et al. 2006

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“…Both labels have been extensively used to study the molecular dynamics of many proteins [17][18][19][20][21] because the spin label can be considered as an additional amino acid residue [22]. In this way, the ESR spectrum of the spin label covalently bound to the protein can reflect either the motion of the macromolecule itself or some residual motion of the label with respect to the whole macromolecule.…”

Section: Introductionmentioning

confidence: 99%

Azurin-Solvent interaction: an ESR spin labeling investigation

Guzzi

Sportelli

1995

Appl. Magn. Reson.

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We have investigated by means of electron spin resonance (ESR) spectroscopy using two spin labels, Iodoacetamido-proxyl and 3-Maleimido-proxyl, the dynamics of two different regions around the active site of azufin, a copper containing blue protein. The ESR measurements of spin labeled azufin have been carried out in the 110-300 K temperature range on wet (H20, D20 and ethanol/water mixtutes) and lyophilized samples. The behaviours of the outer hyperfme splitting separation, 2A', of the ESR spectra vs temperature of the lyophilized, and fully hydrated azurin in H20 and D20 suggest that the two spin labels ate located in regions of the protein surface with different dynamics and polarity. Moreover, all differences in the 2,4" values shown by the spin labeled azufin in normal and heavy water as well as the temperature behaviour disappear when azufin is in ethanol/water mixtares. The results ate discussed in temas of a close correlation between the molecular dynamics of the protein fragments to which the two spin labels are bound and the properties of the solvent used.

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Biomembrane Reactions of Nitroxyl Radicals

Kocherginsky

1992

Bioactive Spin Labels

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Spin labelling of human erythrocytes with nitroxide radicals

Cited by 4 publications

References 24 publications

Circulating Blood Volume Determination Using Electronic Spin Resonance Spectroscopy

Circulating Blood Volume Determination Using Electronic Spin Resonance Spectroscopy

Azurin-Solvent interaction: an ESR spin labeling investigation

Biomembrane Reactions of Nitroxyl Radicals

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