The spider family Linyphiidae is poorly known from many countries in South America, including Peru. In this paper wedescribe a new species of Asemostera Simon, 1898, Asemostera dianae new species, from Madre de Dios, the males ofMeioneta lauta Millidge, 1991 and Meioneta oculata Millidge, 1991, and the female of Myrmecomelix pulcher (Millidge,1991). New occurrence records for Peru include Exechopsis conspicua Millidge, 1991 (known from Brazil), andNovafrontina uncata (F. O. P.-Cambridge, 1902) (known from Brazil, Colombia, Mexico, Panama, and Venezuela). Newdistribution records within Peru for endemic species include Asemostera janetae Miller, 2007, Lygarina finitima Millidge,1991, Meioneta propinqua Millidge, 1991, Meioneta silvae Millidge, 1991 and Sphecozone niwina (Chamberlin, 1916).Illustrations are provided for Asemostera dianae new species and for the undescribed sexes of previously described taxa. Known distributional records are also provided for the above discussed taxa.