Spider toxin peptide lycosin-i functionalized gold nanoparticles for in vivo tumor targeting and therapy

Tan, Hao; Huang, Yazhou; Xu, Jianbing; Chen, Bo; Zhang, Peng; Ye, Zhongju; Liang, Songping; Xiao, Lehui; Z, Liu

doi:10.1016/j.toxicon.2018.10.013

Cited by 9 publications

(11 citation statements)

References 23 publications

(24 reference statements)

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“…Current studies have found that lycosin-I, a peptide toxin isolated from cryptotanneus venom, not only has antimicrobial activity against bacteria and fungi but also induces apoptosis in cancer cells. 27 However, our studies found that different concentrations of lycosin-I had different effects on tumors, and that low concentrations (5 lM) of lycosin-1 significantly inhibited PC-3 and DU-145 cell invasion and metastasis in the absence of tumor cell apoptosis. Higher doses of lycosin-I (10 lM, 20 lM) caused morphological changes in PC-3 and DU-145 cells.…”

Section: Discussionmentioning

confidence: 61%

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Spider peptide toxin lycosin-I induces apoptosis and inhibits migration of prostate cancer cells

Shen

Xie

et al. 2018

Exp Biol Med (Maywood)

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Spider toxins are molecularly diverse and some display not only a strong antibacterial effect but also exhibit significant inhibition of tumor growth and promote tumor cell apoptosis. The aim of the present investigation was to explore different antitumor effects of the spider peptide toxin lycosin-I through different pathways at different concentrations. It was found that by inactivating STAT3 pathway, high concentrations of lycosin-I induce apoptosis in prostate cancer cells and low concentrations of lycosin-I inhibit the migration of prostate cancer cells. This finding provides favorable evidence for further study of the molecular diversity of spider toxins. Impact statement The spider peptide toxin has become an important research topic. These toxins are molecularly diverse and some display not only a strong antibacterial effect but also exhibit significant inhibition of tumor growth and promote tumor cell apoptosis. Inspired by previous studies, the present study aims to investigate the effects of different concentrations of lycosin-I on the invasiveness and apoptosis of human prostate cancer cells. The findings provide favorable evidence for further study of the molecular diversity of spider toxins.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Spider peptide toxin lycosin-I induces apoptosis and inhibits migration of prostate cancer cells

Shen

Xie

et al. 2018

Exp Biol Med (Maywood)

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“…When conjugated to spherical gold nanoparticles, lycosin‐I exhibits selective intracellular translocation towards cancer cells but also displays an unprecedented low selectivity over noncancerous cells. Moreover, this conjugate shows an efficient photothermal effect under NIR irradiation, leading to the killing of cancer cells in vivo 73 …”

Section: Cationic Cppsmentioning

confidence: 99%

New generation of cell‐penetrating peptides: Functionality and potential clinical application

Reißmann

Filatova

2021

Journal of Peptide Science

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Cell‐penetrating peptides (CPPs) can transport various cargoes through membranes of live cells. Since the first generations of CPPs suffered from insufficient cell and tissue selectivity, stability against proteases, and escape from endosomes, a new generation of peptides, with optimized properties, was developed. These are either derived from natural sources or created through the combination of multivalent structures. The second method allows achieving high internalization efficiency, high cell and tissue selectivity, and release from endosomes via hybrid structures, combining sequences for endosomal release, homing sequences, and sequences for activation at the target tissue and for local delivery of cargoes. CPPs with innate tumor selectivity include azurin, crotamine, maurocalcine, lycosin‐I, buffalo cathelicidin, and peptide CB5005. Some of them can penetrate the membranes of live cells and influence intracellular signaling pathways, thereby exerting cytotoxic effects against tumor cells. To obtain multilayer penetration and stabilization against proteolytic degradation, as well as for better handling, CPPs are often conjugated to nanoparticles. A special problem for tumor treatment is the efficiency of drug transport through three‐dimensional cell cultures. Therefore, the capability of CPPs to deliver the drug even to the innermost tissues is of crucial importance. Notably, the ability of certain CPPs to penetrate barriers such as skin, the blood‐brain barrier (BBB), and cornea or conjunctiva of eyes enabled the replacement of dangerous and painful injections with soothing sprays, creams, and drops. However, it is difficult to rank the efficacy of CPPs because transport efficiency and tissue selectivity depend not only on the CPP itself but also on the target tissue or organ, as well as on the cargo and method of CPP‐cargo coupling. Therefore, the present review describes some examples of new‐generation CPPs and aims to provide advice on how to find or create the right CPP for a given task.

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“…Noncancerous cells were much less sensitive to lycosin-I possibly because of reduced interaction between lycosin-I and their membrane. 16 Tan et al 35 found that the lycosin-I-conjugated spherical gold nanoparticles (LGNPs) not only exhibited efficient cellular internalization efficiency toward cancer cells but also displayed unprecedented selectivity over noncancerous cells. Although…”

Section: Spider Venoms In Biomedicinementioning

confidence: 99%

“…89,90 Site-directed mutagenesis combined with in silico docking revealed that PcTx1 binds to the acidic pocket of ASIC1a, with residues Trp 24 , Arg 26 and Arg 27 within the βHL being critical for PcTx1 inhibition of the channel. 89 Mutation of Pro 38 to Ala resulted in a fourfold decrease in potency, while substitution of Pro 35 or Lys 36 with → Ala leads to 2.7, 1.8 fold increase in ability to inhibit the rASIC1a channel, respectively. 91 Although crystal structures of the PcTx1 : cASIC1 complex revealed an extensive network of >50 intermolecular contacts, only ∼50% of these interactions are essential for PcTx1 inhibition of rASIC1a.…”

Section: Review Toxicology Researchmentioning

confidence: 99%

Anticancer, antimicrobial, and analgesic activities of spider venoms

Akef

2018

Toxicology Research

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Spider venoms are complex mixtures composed of a variety of compounds, including salts, small organic molecules, peptides, and proteins. But, the venom of a few species is dangerous to humans. High levels of chemical diversity make spider venoms attractive subjects for chemical prospecting. Many spider venom components show potential activity against a wide range of human diseases. However, the development of novel venom-derived therapeutics requires an understanding of their mechanisms of action. This review will highlight the structures, activities and the possible mechanisms of action of spider venoms and their components against cancer, microbial infections, and pain.

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Spider toxin peptide lycosin-i functionalized gold nanoparticles for in vivo tumor targeting and therapy

Cited by 9 publications

References 23 publications

Spider peptide toxin lycosin-I induces apoptosis and inhibits migration of prostate cancer cells

Spider peptide toxin lycosin-I induces apoptosis and inhibits migration of prostate cancer cells

New generation of cell‐penetrating peptides: Functionality and potential clinical application

Anticancer, antimicrobial, and analgesic activities of spider venoms

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